Tianchi Xin, PhD
Cards
About
Research
Publications
2024
Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation
Xin T, Gallini S, Wei H, Gonzalez D, Matte-Martone C, Machida H, Fujiwara H, Pasolli H, Suozzi K, Gonzalez L, Regot S, Greco V. Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation. Nature Cell Biology 2024, 26: 859-867. PMID: 38689013, DOI: 10.1038/s41556-024-01413-y.Peer-Reviewed Original ResearchERK signalingStem cellsSquamous cell carcinomaHair folliclesOncogenic KRAS mutationsCell carcinomaKRAS mutationsSustained ERK activationERK signaling dynamicsOncogenic mutationsOncogenic KRASERK activationStem cell behaviorIntravital imagingAbnormal cell divisionModulates specific featuresKrasG12DTissue deformationSpatiotemporally specific mannerSustained ERK signalingMutationsLiving miceFolliclesTissue disruptionSingle-cell level
2023
Organ function is preserved despite reorganization of niche architecture in the hair follicle
Wei H, Du S, Parksong J, Pasolli H, Matte-Martone C, Regot S, Gonzalez L, Xin T, Greco V. Organ function is preserved despite reorganization of niche architecture in the hair follicle. Cell Stem Cell 2023, 30: 962-972.e6. PMID: 37419106, PMCID: PMC10362479, DOI: 10.1016/j.stem.2023.06.003.Peer-Reviewed Original ResearchConceptsNiche architectureDermal papilla fibroblastsDifferentiated lineagesHair follicle growthStereotypic architectureMultipotent progenitorsEpithelial progenitorsFunctional importanceNicheStem cellsFibroblast nicheProgenitorsPowerful modelIntravital imagingDermal papillaFibroblastsHair folliclesFollicle growthOrgan functionLineagesDifferentiationCrosstalkHairProliferationCellsInjury prevents Ras mutant cell expansion in mosaic skin
Gallini S, Annusver K, Rahman N, Gonzalez D, Yun S, Matte-Martone C, Xin T, Lathrop E, Suozzi K, Kasper M, Greco V. Injury prevents Ras mutant cell expansion in mosaic skin. Nature 2023, 619: 167-175. PMID: 37344586, PMCID: PMC10322723, DOI: 10.1038/s41586-023-06198-y.Peer-Reviewed Original ResearchConceptsWild-type cellsRas family proteinsCell cycle inhibitor p21Family proteinsOncogenic RasGenetic approachesMosaic tissuesInhibition of EGFRInhibitor p21EGFR ligandsEGFR pathwayCell expansionAberrant growthConstitutive lossDifferential activationParacrine secretionAbsence of injuryCellsCompetitive balanceInjury repairHealthy skinInjurySkinProteinPathwayLive imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
2016
MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis
Yang H, Li M, Hu X, Xin T, Zhang S, Zhao G, Xuan T, Li M. MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis. Developmental Biology 2016, 416: 312-323. PMID: 27339292, DOI: 10.1016/j.ydbio.2016.06.026.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCytoskeletonDrosophila melanogasterDrosophila ProteinsEmbryonic Germ CellsFemaleGene Expression Regulation, DevelopmentalGene Knockdown TechniquesLarvaLuminescent ProteinsMicroRNAsMicroscopy, FluorescenceOrganogenesisOvaryRibonuclease IIIRNA InterferenceRNA-Binding ProteinsStem Cell NicheConceptsOvary morphogenesisPrimordial germ cellsLate third larval instarLoss of DroshaMiRNA pathway componentsCanonical miRNA pathwayGerm cell lineageMiRNA-mediated regulationGerm cell precursorsGenome-wide screeningTerminal filamentThird larval instarEarly larval stagesMiR-317Ovarian somaMiR-14MiR-8Argonaute 1Mutant phenotypeDicer-1MiRNA pathwayPGC differentiationGerm lineGSC nicheRegulatory networks
2014
Evidence for Chromatin-Remodeling Complex PBAP-Controlled Maintenance of the Drosophila Ovarian Germline Stem Cells
He J, Xuan T, Xin T, An H, Wang J, Zhao G, Li M. Evidence for Chromatin-Remodeling Complex PBAP-Controlled Maintenance of the Drosophila Ovarian Germline Stem Cells. PLOS ONE 2014, 9: e103473. PMID: 25068272, PMCID: PMC4113433, DOI: 10.1371/journal.pone.0103473.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell Cycle ProteinsCell DifferentiationChromatin Assembly and DisassemblyChromosomal Proteins, Non-HistoneDrosophila melanogasterDrosophila ProteinsFemaleGene ExpressionGreen Fluorescent ProteinsMicroscopy, ConfocalMutationOvaryOvumProtein BindingReverse Transcriptase Polymerase Chain ReactionRNA InterferenceTrans-ActivatorsTranscription FactorsConceptsGermline stem cellsGermline differentiationFate regulationDrosophila ovarian germline stem cellsSWI/SNF chromatin-remodeling complexOvarian germline stem cellsSWI/SNF complexStem cell fate regulationComplex-specific subunitsChromatin-remodeling complexCell fate regulationGenetic interaction testsStem cellsGSC fateGSC lossBAP complexDrosophila oogenesisMutant phenotypeSNF complexGSC maintenanceBRM functionRegulatory machineryEpigenetic regulationProtein complexesATPase subunits
2013
The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche
Xin T, Xuan T, Tan J, Li M, Zhao G, Li M. The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche. Developmental Biology 2013, 384: 1-12. PMID: 24120347, DOI: 10.1016/j.ydbio.2013.10.001.Peer-Reviewed Original ResearchConceptsGermline stem cellsGSC maintenanceFate regulationEpigenetic mechanismsDrosophila ovarian germline stem cellsStem cellsOvarian germline stem cellsFemale germline stem cellsStem cell fate regulationCell fate regulationRNA binding proteinCell-autonomous roleAdult stem cellsGSC lossPutative histoneEnokEpigenetic regulationNiche maintenanceDaughter cellsHistone acetyltransferaseMaintenance defectsEctopic expressionMutant allelesBinding proteinMolecular studiesdBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary
Xuan T, Xin T, He J, Tan J, Gao Y, Feng S, He L, Zhao G, Li M. dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary. Developmental Biology 2013, 379: 167-181. PMID: 23624310, DOI: 10.1016/j.ydbio.2013.04.015.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosis Regulatory ProteinsCell DifferentiationDNA PrimersDrosophila melanogasterDrosophila ProteinsEpigenesis, GeneticFemaleGerm CellsHistone MethyltransferasesHistone-Lysine N-MethyltransferaseHistonesMethylationMicroscopy, FluorescenceOvaryReal-Time Polymerase Chain ReactionRNA InterferenceStatistics, NonparametricStem Cell NicheStem CellsUbiquitin-Protein LigasesConceptsGermline stem cellsGerm cell differentiationStem cell nicheCell differentiationDrosophila ovaryGSC maintenanceDrosophila ovarian germline stem cellsCell nicheGermline stem cell maintenanceOvarian germline stem cellsCell fate regulationStem cell maintenanceHistone H3K4 trimethylationE3 ubiquitin ligaseStem cellsSingle germ cellEscort cellsGSC lossGSC regulationChromatin remodelingMutant ovariesH3K4 methylationHistone modificationsFate regulationH3K4 trimethylation
2010
Activation of JNK signaling links lgl mutations to disruption of the cell polarity and epithelial organization in Drosophila imaginal discs
Zhu M, Xin T, Weng S, Gao Y, Zhang Y, Li Q, Li M. Activation of JNK signaling links lgl mutations to disruption of the cell polarity and epithelial organization in Drosophila imaginal discs. Cell Research 2010, 20: 242-245. PMID: 20066009, DOI: 10.1038/cr.2010.2.Peer-Reviewed Original Research
2009
Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis
Li Q, Shen L, Xin T, Xiang W, Chen W, Gao Y, Zhu M, Yu L, Li M. Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis. BMC Developmental Biology 2009, 9: 60. PMID: 19948068, PMCID: PMC2810132, DOI: 10.1186/1471-213x-9-60.Peer-Reviewed Original ResearchConceptsTumor suppressor geneDlg functionFate inductionPosterior patterningCell fateEgg chambersDrosophila tumor suppressor geneSuppressor geneBorder cell fateFollicle cellsCell fate inductionAnterior-posterior patterningDrosophila egg developmentRole of ScribFollicle cell epitheliumFurther genetic analysisMultiple signaling pathwaysDistinct cell typesAnterior-posterior axisDrosophila oogenesisEpithelial polarityEpithelial patterningMosaic clonesFollicular epitheliumAnterior domain