Jaime Grutzendler, MD
Research & Publications
Biography
News
Research Summary
The dynamics and logic of neuroglial interactions in neurodegenerative pathologies
The overall goal of our laboratory is to uncover rules that govern the complex interactions between all brain cell types in their unperturbed in vivo microenvironment and to determine how these dynamic cell-cell interactions are disrupted in a variety of disease states. We utilize high-resolution cellular imaging in vivo and fixed tissues of single cells and small clusters of interacting cells combined with novel optical sensors of cellular physiology, optogenetics, chemogenetics, genome editing techniques and methods that we develop for improving the capacity to visualize and manipulate individual cells in their native environment. Our approach tends to be exploratory and hypothesis generating and thus, our results can take us in different directions such that projects in the lab can have a neuronal, glial or vascular focus. Finally, discoveries derived from these investigative strategies inspire our translational neuroscience program aimed at neurodegenerative conditions.
Extensive Research Description
- Cellular and molecular mechanisms of neurodegeneration: Neurodegenerative diseases such as Alzheimer’s are the result of complex and multicellular age-related cellular processes that disrupt normal cellular functions and neuroglial interactions. This eventually results in disruption of intercellular communication, loss of synapses and cell death. We are interested in various components of neurodegeneration including: 1) axonal disruption in Alzheimer’s disease; 2) age-related myelin degeneration; 3) neuroprotective roles of glial cells during amyloid deposition; 4) mechanisms of cell death and corpse removal by glial cells; 5) mechanism of disruption of gliovascular interactions.
- Development of methods for intravital imaging and targeted cellular manipulation: In order to obtain a comprehensive understanding of the dynamics of neurodegenerative processes, we develop and implement a variety of methodologies for high-resolution in vivo optical brain imaging and novel methods for cell-specific labeling and manipulation. Examples of techniques that we have developed include: 1) spectral confocal reflectance microscopy (SCoRe) for high-resolution intravital label-free imaging of myelinated axons; 2) Two photon targeted chemical-apoptotic ablation (2Phatal) of cells in vivo to understand the dynamics of glial interactions with dying cells; 3) discovery of small fluorescent molecules for cell specific labeling during intravital imaging.
- Translational neuroscience: we have active efforts at developing neurotherapeutics within the field of neurodegeneration and nervous system injury. One goal is the development of treatments with cellular specificity. We have and ongoing program involving collaboration with chemists for the development of small molecules with unique properties of crossing the blood brain barrier and entering specific neural cell types to delivering therapeutic cargos. In addition, we are exploring the use of novel biologicals (antibodies and antisense oligos). We aim to apply these therapeutic agents in preclinical studies to target some of the pathological features of degeneration (axonal, myelin, microglia and vascular) that we have uncovered.
Coauthors
Research Interests
Alzheimer Disease; Astrocytes; Axons; Blood-Brain Barrier; Capillaries; Cerebrovascular Circulation; Microscopy; Nerve Fibers, Myelinated; Neuronal Plasticity; Regional Blood Flow; Microglia; Neurodegenerative Diseases; Pericytes
Selected Publications
- PLD3 affects axonal spheroids and network defects in Alzheimer’s diseaseYuan P, Zhang M, Tong L, Morse T, McDougal R, Ding H, Chan D, Cai Y, Grutzendler J. PLD3 affects axonal spheroids and network defects in Alzheimer’s disease. Nature 2022, 612: 328-337. PMID: 36450991, PMCID: PMC9729106, DOI: 10.1038/s41586-022-05491-6.
- Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivoDamisah EC, Hill RA, Rai A, Chen F, Rothlin CV, Ghosh S, Grutzendler J. Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo. Science Advances 2020, 6: eaba3239. PMID: 32637606, PMCID: PMC7319765, DOI: 10.1126/sciadv.aba3239.
- Targeted two-photon chemical apoptotic ablation of defined cell types in vivoHill RA, Damisah EC, Chen F, Kwan AC, Grutzendler J. Targeted two-photon chemical apoptotic ablation of defined cell types in vivo. Nature Communications 2017, 8: 15837. PMID: 28621306, PMCID: PMC5501159, DOI: 10.1038/ncomms15837.
- Microglia-Mediated Neuroprotection, TREM2, and Alzheimer’s Disease: Evidence From Optical ImagingCondello C, Yuan P, Grutzendler J. Microglia-Mediated Neuroprotection, TREM2, and Alzheimer’s Disease: Evidence From Optical Imaging. Biological Psychiatry 2017, 83: 377-387. PMID: 29169609, PMCID: PMC5767550, DOI: 10.1016/j.biopsych.2017.10.007.
- TREM2 Haplodeficiency in Mice and Humans Impairs the Microglia Barrier Function Leading to Decreased Amyloid Compaction and Severe Axonal DystrophyYuan P, Condello C, Keene CD, Wang Y, Bird TD, Paul SM, Luo W, Colonna M, Baddeley D, Grutzendler J. TREM2 Haplodeficiency in Mice and Humans Impairs the Microglia Barrier Function Leading to Decreased Amyloid Compaction and Severe Axonal Dystrophy. Neuron 2016, 90: 724-739. PMID: 27196974, PMCID: PMC4898967, DOI: 10.1016/j.neuron.2016.05.003.
- A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imagingDamisah EC, Hill RA, Tong L, Murray KN, Grutzendler J. A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imaging. Nature Neuroscience 2017, 20: 1023-1032. PMID: 28504673, PMCID: PMC5550770, DOI: 10.1038/nn.4564.
- Imaging and optogenetic modulation of vascular mural cells in the live brainTong L, Hill RA, Damisah EC, Murray KN, Yuan P, Bordey A, Grutzendler J. Imaging and optogenetic modulation of vascular mural cells in the live brain. Nature Protocols 2020, 16: 472-496. PMID: 33299155, DOI: 10.1038/s41596-020-00425-w.
- Lifelong cortical myelin plasticity and age-related degeneration in the live mammalian brainHill RA, Li AM, Grutzendler J. Lifelong cortical myelin plasticity and age-related degeneration in the live mammalian brain. Nature Neuroscience 2018, 21: 683-695. PMID: 29556031, PMCID: PMC5920745, DOI: 10.1038/s41593-018-0120-6.
- Long-term dendritic spine stability in the adult cortexGrutzendler J, Kasthuri N, Gan WB. Long-term dendritic spine stability in the adult cortex. Nature 2002, 420: 812-816. PMID: 12490949, DOI: 10.1038/nature01276.
- ATP mediates rapid microglial response to local brain injury in vivoDavalos D, Grutzendler J, Yang G, Kim JV, Zuo Y, Jung S, Littman DR, Dustin ML, Gan WB. ATP mediates rapid microglial response to local brain injury in vivo. Nature Neuroscience 2005, 8: 752-758. PMID: 15895084, DOI: 10.1038/nn1472.
- TREM2 Haplodeficiency in Mice and Humans Impairs the Microglia Barrier Function Leading to Decreased Amyloid Compaction and Severe Axonal DystrophyYuan P, Condello C, Keene CD, Wang Y, Bird TD, Paul SM, Luo W, Colonna M, Baddeley D, Grutzendler J. TREM2 Haplodeficiency in Mice and Humans Impairs the Microglia Barrier Function Leading to Decreased Amyloid Compaction and Severe Axonal Dystrophy. Neuron 2016, 92: 252-264. PMID: 27710785, DOI: 10.1016/j.neuron.2016.09.016.
- Microglia constitute a barrier that prevents neurotoxic protofibrillar Aβ42 hotspots around plaquesCondello C, Yuan P, Schain A, Grutzendler J. Microglia constitute a barrier that prevents neurotoxic protofibrillar Aβ42 hotspots around plaques. Nature Communications 2015, 6: 6176. PMID: 25630253, PMCID: PMC4311408, DOI: 10.1038/ncomms7176.
- Angiophagy Prevents Early Embolus Washout But Recanalizes Microvessels Through Embolus ExtravasationGrutzendler J, Murikinati S, Hiner B, Ji L, Lam CK, Yoo T, Gupta S, Hafler BP, Adelman RA, Yuan P, Rodriguez G. Angiophagy Prevents Early Embolus Washout But Recanalizes Microvessels Through Embolus Extravasation. Science Translational Medicine 2014, 6: 226ra31. PMID: 24598589, DOI: 10.1126/scitranslmed.3006585.
- Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopySchain AJ, Hill RA, Grutzendler J. Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy. Nature Medicine 2014, 20: 443-449. PMID: 24681598, PMCID: PMC3981936, DOI: 10.1038/nm.3495.
- In vivo imaging of oligodendrocytes with sulforhodamine 101Hill RA, Grutzendler J. In vivo imaging of oligodendrocytes with sulforhodamine 101. Nature Methods 2014, 11: 1081-1082. PMID: 25357236, PMCID: PMC4539948, DOI: 10.1038/nmeth.3140.
- AngiophagyGrutzendler J. Angiophagy. Stroke 2013, 44: s84-s86. PMID: 23709741, DOI: 10.1161/strokeaha.112.678730.
- Embolus extravasation is an alternative mechanism for cerebral microvascular recanalizationLam CK, Yoo T, Hiner B, Liu Z, Grutzendler J. Embolus extravasation is an alternative mechanism for cerebral microvascular recanalization. Nature 2010, 465: 478-482. PMID: 20505729, PMCID: PMC2879083, DOI: 10.1038/nature09001.
- Regional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary PericytesHill RA, Tong L, Yuan P, Murikinati S, Gupta S, Grutzendler J. Regional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes. Neuron 2015, 87: 95-110. PMID: 26119027, PMCID: PMC4487786, DOI: 10.1016/j.neuron.2015.06.001.
- Single cell in vivo optogenetic stimulation by two-photon excitation fluorescence transferTong L, Han S, Xue Y, Chen M, Chen F, Ke W, Shu Y, Ding N, Bewersdorf J, Zhou Z, Yuan P, Grutzendler J. Single cell in vivo optogenetic stimulation by two-photon excitation fluorescence transfer. IScience 2023, 26: 107857. PMID: 37752954, PMCID: PMC10518705, DOI: 10.1016/j.isci.2023.107857.
- Oligodendroglial macroautophagy is essential for myelin sheath turnover to prevent neurodegeneration and deathAber ER, Griffey CJ, Davies T, Li AM, Yang YJ, Croce KR, Goldman JE, Grutzendler J, Canman JC, Yamamoto A. Oligodendroglial macroautophagy is essential for myelin sheath turnover to prevent neurodegeneration and death. Cell Reports 2022, 41: 111480. PMID: 36261002, PMCID: PMC9639605, DOI: 10.1016/j.celrep.2022.111480.
- KCNJ8/ABCC9-containing K-ATP channel modulates brain vascular smooth muscle development and neurovascular couplingAndo K, Tong L, Peng D, Vázquez-Liébanas E, Chiyoda H, He L, Liu J, Kawakami K, Mochizuki N, Fukuhara S, Grutzendler J, Betsholtz C. KCNJ8/ABCC9-containing K-ATP channel modulates brain vascular smooth muscle development and neurovascular coupling. Developmental Cell 2022, 57: 1383-1399.e7. PMID: 35588738, DOI: 10.1016/j.devcel.2022.04.019.
- Intravital Imaging of Neocortical Heterotopia Reveals Aberrant Axonal Pathfinding and Myelination around Ectopic NeuronsLi AM, Hill RA, Grutzendler J. Intravital Imaging of Neocortical Heterotopia Reveals Aberrant Axonal Pathfinding and Myelination around Ectopic Neurons. Cerebral Cortex 2021, 31: bhab090-. PMID: 33877363, PMCID: PMC8328209, DOI: 10.1093/cercor/bhab090.
- 3D super-resolution deep-tissue imaging in living miceVelasco MGM, Zhang M, Antonello J, Yuan P, Allgeyer ES, May D, M’Saad O, Kidd P, Barentine AES, Greco V, Grutzendler J, Booth MJ, Bewersdorf J. 3D super-resolution deep-tissue imaging in living mice. Optica 2021, 8: 442-450. PMID: 34239948, PMCID: PMC8243577, DOI: 10.1364/optica.416841.
- Unlocking Pericyte Function in the Adult Blood Brain Barrier One Cell at a TimeNicoli S, Grutzendler J. Unlocking Pericyte Function in the Adult Blood Brain Barrier One Cell at a Time. Circulation Research 2021, 128: 511-512. PMID: 33600230, PMCID: PMC7928238, DOI: 10.1161/circresaha.121.318799.
- Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse modelZhou HJ, Qin L, Jiang Q, Murray KN, Zhang H, Li B, Lin Q, Graham M, Liu X, Grutzendler J, Min W. Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model. Nature Communications 2021, 12: 504. PMID: 33495460, PMCID: PMC7835246, DOI: 10.1038/s41467-020-20774-0.
- TREM2: Modulator of Lipid Metabolism in MicrogliaDamisah EC, Rai A, Grutzendler J. TREM2: Modulator of Lipid Metabolism in Microglia. Neuron 2020, 105: 759-761. PMID: 32135085, DOI: 10.1016/j.neuron.2020.02.008.
- Emerging technologies to study glial cellsHirbec H, Déglon N, Foo LC, Goshen I, Grutzendler J, Hangen E, Kreisel T, Linck N, Muffat J, Regio S, Rion S, Escartin C. Emerging technologies to study glial cells. Glia 2020, 68: 1692-1728. PMID: 31958188, DOI: 10.1002/glia.23780.
- Cellular Control of Brain Capillary Blood Flow: In Vivo Imaging VeritasGrutzendler J, Nedergaard M. Cellular Control of Brain Capillary Blood Flow: In Vivo Imaging Veritas. Trends In Neurosciences 2019, 42: 528-536. PMID: 31255380, PMCID: PMC7386067, DOI: 10.1016/j.tins.2019.05.009.
- Uncovering the biology of myelin with optical imaging of the live brainHill RA, Grutzendler J. Uncovering the biology of myelin with optical imaging of the live brain. Glia 2019, 67: 2008-2019. PMID: 31033062, PMCID: PMC6744352, DOI: 10.1002/glia.23635.
- Publisher Correction: Flexible Learning-Free Segmentation and Reconstruction of Neural VolumesShahbazi A, Kinnison J, Vescovi R, Du M, Hill R, Joesch M, Takeno M, Zeng H, da Costa NM, Grutzendler J, Kasthuri N, Scheirer WJ. Publisher Correction: Flexible Learning-Free Segmentation and Reconstruction of Neural Volumes. Scientific Reports 2018, 8: 17585. PMID: 30498261, PMCID: PMC6265300, DOI: 10.1038/s41598-018-36220-7.
- Flexible Learning-Free Segmentation and Reconstruction of Neural VolumesShahbazi A, Kinnison J, Vescovi R, Du M, Hill R, Joesch M, Takeno M, Zeng H, da Costa NM, Grutzendler J, Kasthuri N, Scheirer WJ. Flexible Learning-Free Segmentation and Reconstruction of Neural Volumes. Scientific Reports 2018, 8: 14247. PMID: 30250218, PMCID: PMC6155135, DOI: 10.1038/s41598-018-32628-3.
- Activation of pial and dural macrophages and dendritic cells by cortical spreading depressionSchain AJ, Melo‐Carrillo A, Borsook D, Grutzendler J, Strassman AM, Burstein R. Activation of pial and dural macrophages and dendritic cells by cortical spreading depression. Annals Of Neurology 2018, 83: 508-521. PMID: 29394508, PMCID: PMC5965700, DOI: 10.1002/ana.25169.
- Oxalate-curcumin–based probe for micro- and macroimaging of reactive oxygen species in Alzheimer’s diseaseYang J, Zhang X, Yuan P, Yang J, Xu Y, Grutzendler J, Shao Y, Moore A, Ran C. Oxalate-curcumin–based probe for micro- and macroimaging of reactive oxygen species in Alzheimer’s disease. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 12384-12389. PMID: 29109280, PMCID: PMC5703278, DOI: 10.1073/pnas.1706248114.
- “Small Blood Vessels: Big Health Problems?”: Scientific Recommendations of the National Institutes of Health WorkshopBosetti F, Galis Z, Bynoe M, Charette M, Cipolla M, del Zoppo G, Gould D, Hatsukami T, Jones T, Koenig J, Lutty G, Maric‐Bilkan C, Stevens T, Tolunay H, Koroshetz W, Participants T, Agalliu D, Antonetti D, Boehm M, Brooks C, Caron K, Chilian W, Daemen M, D'Amato R, Davis T, Ergul A, Faber J, Gomez A, Grayson P, Grumbach I, Grutzendler J, Gu C, Gutterman D, Hallenbeck J, Herman I, Humphrey J, Iadecola C, Inscho E, Kleinfeld D, Lo E, Lopez J, Macknik S, Malik A, Mayadas T, McGavern D, Meininger G, Miller V, Nedergaard M, Nelson M, Peirce‐Cottler S, Ramadan I, Rosenberg G, Schiffrin E, Searson P, Stachenfeld N, Stan R, Suarez Y, Ubogu E, Vexler Z, Weyand C, Zlokovic B. “Small Blood Vessels: Big Health Problems?”: Scientific Recommendations of the National Institutes of Health Workshop. Journal Of The American Heart Association 2016, 5: e004389. PMID: 27815267, PMCID: PMC5210346, DOI: 10.1161/jaha.116.004389.
- O2‐07‐02: Trem2‐Mediated Early Response by Resident Microglia Limits Diffusion and Toxicity of Amyloid PlaquesWang Y, Ulland T, Ulrich J, Song W, Yuan P, Mahan T, Shi Y, Gilfillan S, Cella M, DeMattos R, Grutzendler J, Cirrito J, Holtzman D, Colonna M. O2‐07‐02: Trem2‐Mediated Early Response by Resident Microglia Limits Diffusion and Toxicity of Amyloid Plaques. Alzheimer's & Dementia 2016, 12: p241-p242. DOI: 10.1016/j.jalz.2016.06.431.
- O1‐12‐04: Near Infrared Fluorescence Imaging of Reactive Oxygen Species in Alzheimer’s Disease Via Transformation From “Visible” to “Invisible”Ran C, Yang J, Yang J, Yuan P, Grutzendler J, Xu Y. O1‐12‐04: Near Infrared Fluorescence Imaging of Reactive Oxygen Species in Alzheimer’s Disease Via Transformation From “Visible” to “Invisible”. Alzheimer's & Dementia 2016, 12: p206-p206. DOI: 10.1016/j.jalz.2016.06.361.
- TREM2-mediated early microglial response limits diffusion and toxicity of amyloid plaquesWang Y, Ulland TK, Ulrich JD, Song W, Tzaferis JA, Hole JT, Yuan P, Mahan TE, Shi Y, Gilfillan S, Cella M, Grutzendler J, DeMattos RB, Cirrito JR, Holtzman DM, Colonna M. TREM2-mediated early microglial response limits diffusion and toxicity of amyloid plaques. Journal Of Experimental Medicine 2016, 213: 667-675. PMID: 27091843, PMCID: PMC4854736, DOI: 10.1084/jem.20151948.
- Increased Nanoparticle Delivery to Brain Tumors by Autocatalytic Priming for Improved Treatment and ImagingHan L, Kong DK, Zheng MQ, Murikinati S, Ma C, Yuan P, Li L, Tian D, Cai Q, Ye C, Holden D, Park JH, Gao X, Thomas JL, Grutzendler J, Carson RE, Huang Y, Piepmeier JM, Zhou J. Increased Nanoparticle Delivery to Brain Tumors by Autocatalytic Priming for Improved Treatment and Imaging. ACS Nano 2016, 10: 4209-4218. PMID: 26967254, PMCID: PMC5257033, DOI: 10.1021/acsnano.5b07573.
- Neurovascular and Immuno-Imaging: From Mechanisms to Therapies. Proceedings of the Inaugural SymposiumAkassoglou K, Agalliu D, Chang CJ, Davalos D, Grutzendler J, Hillman EM, Khakh BS, Kleinfeld D, McGavern DB, Nelson SJ, Zlokovic BV. Neurovascular and Immuno-Imaging: From Mechanisms to Therapies. Proceedings of the Inaugural Symposium. Frontiers In Neuroscience 2016, 10: 46. PMID: 26941593, PMCID: PMC4761864, DOI: 10.3389/fnins.2016.00046.
- Attenuation of β-Amyloid Deposition and Neurotoxicity by Chemogenetic Modulation of Neural ActivityYuan P, Grutzendler J. Attenuation of β-Amyloid Deposition and Neurotoxicity by Chemogenetic Modulation of Neural Activity. Journal Of Neuroscience 2016, 36: 632-641. PMID: 26758850, PMCID: PMC4710779, DOI: 10.1523/jneurosci.2531-15.2016.
- Label-free Confocal Reflectance and 2-photon Microscopy of Myelinated Axons and Microvasculature in Live MiceGrutzendler J. Label-free Confocal Reflectance and 2-photon Microscopy of Myelinated Axons and Microvasculature in Live Mice. 2016, bw4b.2. DOI: 10.1364/brain.2016.bw4b.2.
- Absolute two-photon excitation spectra of red and far-red fluorescent probes.Velasco MG, Allgeyer ES, Yuan P, Grutzendler J, Bewersdorf J. Absolute two-photon excitation spectra of red and far-red fluorescent probes. Optics Letters 2015, 40: 4915-8. PMID: 26512482, DOI: 10.1364/ol.40.004915.
- Massive accumulation of luminal protease-deficient axonal lysosomes at Alzheimer’s disease amyloid plaquesGowrishankar S, Yuan P, Wu Y, Schrag M, Paradise S, Grutzendler J, De Camilli P, Ferguson SM. Massive accumulation of luminal protease-deficient axonal lysosomes at Alzheimer’s disease amyloid plaques. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: e3699-e3708. PMID: 26124111, PMCID: PMC4507205, DOI: 10.1073/pnas.1510329112.
- Genetic variants associated with autoimmunity drive NFκB signaling and responses to inflammatory stimuliHousley WJ, Fernandez SD, Vera K, Murikinati SR, Grutzendler J, Cuerdon N, Glick L, De Jager PL, Mitrovic M, Cotsapas C, Hafler DA. Genetic variants associated with autoimmunity drive NFκB signaling and responses to inflammatory stimuli. Science Translational Medicine 2015, 7: 291ra93. PMID: 26062845, PMCID: PMC4574294, DOI: 10.1126/scitranslmed.aaa9223.
- A bifunctional curcumin analogue for two-photon imaging and inhibiting crosslinking of amyloid beta in Alzheimer's diseaseZhang X, Tian Y, Yuan P, Li Y, Yaseen MA, Grutzendler J, Moore A, Ran C. A bifunctional curcumin analogue for two-photon imaging and inhibiting crosslinking of amyloid beta in Alzheimer's disease. Chemical Communications 2014, 50: 11550-11553. PMID: 25134928, PMCID: PMC4617557, DOI: 10.1039/c4cc03731f.
- Modulation of oligodendrocyte generation during a critical temporal window after NG2 cell divisionHill RA, Patel KD, Goncalves CM, Grutzendler J, Nishiyama A. Modulation of oligodendrocyte generation during a critical temporal window after NG2 cell division. Nature Neuroscience 2014, 17: 1518-1527. PMID: 25262495, PMCID: PMC4275302, DOI: 10.1038/nn.3815.
- Perturbed neural activity disrupts cerebral angiogenesis during a postnatal critical periodWhiteus C, Freitas C, Grutzendler J. Perturbed neural activity disrupts cerebral angiogenesis during a postnatal critical period. Nature 2013, 505: 407-411. PMID: 24305053, PMCID: PMC3947100, DOI: 10.1038/nature12821.
- P1–033: A time stamp imaging method for measuring the kinetics and toxicity of beta‐amyloid and tau deposition by sequential multicolor labeling with beta‐sheet binding dyesYuan P, Condello C, Schain A, Grutzendler J. P1–033: A time stamp imaging method for measuring the kinetics and toxicity of beta‐amyloid and tau deposition by sequential multicolor labeling with beta‐sheet binding dyes. Alzheimer's & Dementia 2013, 9: p164-p164. DOI: 10.1016/j.jalz.2013.05.253.
- P2–018: Microglia constitute a barrier that prevents the formation of neurotoxic oligomeric beta‐amyloid hot spots around plaquesCondello C, Yuan P, Das P, Grutzendler J. P2–018: Microglia constitute a barrier that prevents the formation of neurotoxic oligomeric beta‐amyloid hot spots around plaques. Alzheimer's & Dementia 2013, 9: p351-p351. DOI: 10.1016/j.jalz.2013.05.660.
- In Vivo Imaging of Cerebral Microvascular Plasticity from Birth to DeathHarb R, Whiteus C, Freitas C, Grutzendler J. In Vivo Imaging of Cerebral Microvascular Plasticity from Birth to Death. Cerebrovascular And Brain Metabolism Reviews 2012, 33: 146-156. PMID: 23093067, PMCID: PMC3597363, DOI: 10.1038/jcbfm.2012.152.
- Transcranial Two-Photon Imaging of the Living Mouse BrainGrutzendler J, Yang G, Pan F, Parkhurst CN, Gan WB. Transcranial Two-Photon Imaging of the Living Mouse Brain. Cold Spring Harbor Protocols 2011, 2011: pdb.prot065474. PMID: 21880826, PMCID: PMC4641516, DOI: 10.1101/pdb.prot065474.
- Multicolor time-stamp reveals the dynamics and toxicity of amyloid depositionCondello C, Schain A, Grutzendler J. Multicolor time-stamp reveals the dynamics and toxicity of amyloid deposition. Scientific Reports 2011, 1: 19. PMID: 22355538, PMCID: PMC3216507, DOI: 10.1038/srep00019.
- CX3CR1 in Microglia Regulates Brain Amyloid Deposition through Selective Protofibrillar Amyloid-β PhagocytosisLiu Z, Condello C, Schain A, Harb R, Grutzendler J. CX3CR1 in Microglia Regulates Brain Amyloid Deposition through Selective Protofibrillar Amyloid-β Phagocytosis. Journal Of Neuroscience 2010, 30: 17091-17101. PMID: 21159979, PMCID: PMC3077120, DOI: 10.1523/jneurosci.4403-10.2010.
- Thinned-skull cranial window technique for long-term imaging of the cortex in live miceYang G, Pan F, Parkhurst CN, Grutzendler J, Gan WB. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice. Nature Protocols 2010, 5: 201-208. PMID: 20134419, PMCID: PMC4690457, DOI: 10.1038/nprot.2009.222.
- Ballistic delivery of dyes for structural and functional studies of the nervous system.Gan WB, Grutzendler J, Wong RO, Lichtman JW. Ballistic delivery of dyes for structural and functional studies of the nervous system. Cold Spring Harbor Protocols 2009, 2009: pdb.prot5202. PMID: 20147144, PMCID: PMC2916724, DOI: 10.1101/pdb.prot5202.
- Long-term two-photon transcranial imaging of synaptic structures in the living brain.Grutzendler J, Gan WB. Long-term two-photon transcranial imaging of synaptic structures in the living brain. Cold Spring Harbor Protocols 2007, 2007: pdb.prot4766. PMID: 21357119, DOI: 10.1101/pdb.prot4766.
- Various Dendritic Abnormalities Are Associated with Fibrillar Amyloid Deposits in Alzheimer's DiseaseGRUTZENDLER J, HELMIN K, TSAI J, GAN W. Various Dendritic Abnormalities Are Associated with Fibrillar Amyloid Deposits in Alzheimer's Disease. Annals Of The New York Academy Of Sciences 2007, 1097: 30-39. PMID: 17413007, DOI: 10.1196/annals.1379.003.
- Two-photon imaging of synaptic plasticity and pathology in the living mouse brainGrutzendler J, Gan WB. Two-photon imaging of synaptic plasticity and pathology in the living mouse brain. Neurotherapeutics 2006, 3: 489-496. PMID: 17012063, PMCID: PMC3593400, DOI: 10.1016/j.nurx.2006.07.005.
- Fibrillar amyloid deposition leads to local synaptic abnormalities and breakage of neuronal branchesTsai J, Grutzendler J, Duff K, Gan WB. Fibrillar amyloid deposition leads to local synaptic abnormalities and breakage of neuronal branches. Nature Neuroscience 2004, 7: 1181-1183. PMID: 15475950, DOI: 10.1038/nn1335.
- Optical Imaging of Synaptic Disruption in a Mouse Model of Alzheimer's DiseaseTsai J, Grutzendler J, Gan W. Optical Imaging of Synaptic Disruption in a Mouse Model of Alzheimer's Disease. Microscopy And Microanalysis 2004, 10: 166-167. DOI: 10.1017/s1431927604883740.
- Age‐associated synapse elimination in mouse parasympathetic gangliaCoggan JS, Grutzendler J, Bishop DL, Cook MR, Gan W, Heym J, Lichtman JW. Age‐associated synapse elimination in mouse parasympathetic ganglia. Developmental Neurobiology 2004, 60: 214-226. PMID: 15266652, DOI: 10.1002/neu.20022.
- Rapid labeling of neuronal populations by ballistic delivery of fluorescent dyesGrutzendler J, Tsai J, Gan WB. Rapid labeling of neuronal populations by ballistic delivery of fluorescent dyes. Methods 2003, 30: 79-85. PMID: 12695105, DOI: 10.1016/s1046-2023(03)00009-4.
- Two modes of radial migration in early development of the cerebral cortexNadarajah B, Brunstrom J, Grutzendler J, Wong R, Pearlman A. Two modes of radial migration in early development of the cerebral cortex. Nature Neuroscience 2001, 4: 143-150. PMID: 11175874, DOI: 10.1038/83967.
- Cholinesterase Inhibitors for Alzheimer’s DiseaseGrutzendler J, Morris J. Cholinesterase Inhibitors for Alzheimer’s Disease. Drugs 2001, 61: 41-52. PMID: 11217870, DOI: 10.2165/00003495-200161010-00005.
- Multicolor “DiOlistic” Labeling of the Nervous System Using Lipophilic Dye CombinationsGan W, Grutzendler J, Wong W, Wong R, Lichtman J. Multicolor “DiOlistic” Labeling of the Nervous System Using Lipophilic Dye Combinations. Neuron 2000, 27: 219-225. PMID: 10985343, DOI: 10.1016/s0896-6273(00)00031-3.