2017
Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation
Roy K, Jerman S, Jozsef L, McNamara T, Onyekaba G, Sun Z, Marin EP. Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation. Journal Of Biological Chemistry 2017, 292: 17703-17717. PMID: 28848045, PMCID: PMC5663873, DOI: 10.1074/jbc.m117.792937.Peer-Reviewed Original ResearchConceptsPost-translational attachmentMost mammalian cellsCiliary GTPase Arl13bCilia localizationProtein palmitoylationCiliary proteinsCilia proteinsProtein localizationCilia formationMammalian cellsCilia functionPalmitoylationPrimary ciliaPlasma membraneCilia resorptionArl13bFunctional importanceMyristoylationCiliaCritical roleProteinMouse kidneyLocalizationDepalmitoylationCells
2015
Mother Centriole Distal Appendages Mediate Centrosome Docking at the Immunological Synapse and Reveal Mechanistic Parallels with Ciliogenesis
Stinchcombe J, Randzavola L, Angus K, Mantell J, Verkade P, Griffiths G. Mother Centriole Distal Appendages Mediate Centrosome Docking at the Immunological Synapse and Reveal Mechanistic Parallels with Ciliogenesis. Current Biology 2015, 25: 3239-3244. PMID: 26670998, PMCID: PMC4691242, DOI: 10.1016/j.cub.2015.10.028.Peer-Reviewed Original ResearchConceptsCilia formationSmall interfering RNACentrosome dockingCentrosome polarizationMother centriolePlasma membraneImmunological synapseBasal body dockingDistal appendage proteinsCytotoxic T lymphocytesPrimary cilia formationAppendage proteinsSecretory lysosomesPolarized secretionDistal appendagesCentriole pairCell cycleCiliogenesisCentrosomeMechanistic parallelsMembrane contactSynapse formationPolarized releaseCentriolesSynapse
2013
Hedgehog Signaling Controls T Cell Killing at the Immunological Synapse
de la Roche M, Ritter A, Angus K, Dinsmore C, Earnshaw C, Reiter J, Griffiths G. Hedgehog Signaling Controls T Cell Killing at the Immunological Synapse. Science 2013, 342: 1247-1250. PMID: 24311692, PMCID: PMC4022134, DOI: 10.1126/science.1244689.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesCell PolarityCells, CulturedCentrosomeCytotoxicity, ImmunologicHedgehog ProteinsImmunological SynapsesKruppel-Like Transcription FactorsMiceMice, Inbred BALB CMice, Inbred C57BLMice, TransgenicModels, ImmunologicalNeuropeptidesPatched Receptorsrac1 GTP-Binding ProteinReceptors, Antigen, T-CellReceptors, Cell SurfaceReceptors, G-Protein-CoupledSignal TransductionSmoothened ReceptorT-Lymphocytes, CytotoxicZinc Finger Protein GLI1ConceptsCytotoxic T lymphocytesHh signalingImmunological synapseVertebrate Hedgehog (Hh) signalingPlasma membraneHedgehog (Hh) signalingHh pathway activationT cell receptor activationCytotoxic granulesActin remodelingCentrosome polarizationCytotoxic T lymphocyte functionCytotoxic T lymphocyte killingCilia formationPrimary ciliaT cell killingCentrosome dockingCentrosomeModified ciliumPathway activationGranule releaseNonhematopoietic cellsT lymphocytesReceptor activationCilia
2012
Arl13b regulates endocytic recycling traffic
Barral D, Garg S, Casalou C, Watts G, Sandoval J, Ramalho J, Hsu V, Brenner M. Arl13b regulates endocytic recycling traffic. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 21354-21359. PMID: 23223633, PMCID: PMC3535586, DOI: 10.1073/pnas.1218272110.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonADP-Ribosylation Factor 6ADP-Ribosylation FactorsAntigens, CD1Cell MembraneCluster AnalysisEndocytosisEndosomesGene SilencingGreen Fluorescent ProteinsHeLa CellsHumansMutant ProteinsProtein BindingProtein Structure, TertiaryProtein Transportrab GTP-Binding ProteinsTransferrinConceptsActin cytoskeletonRecycling trafficRecycling pathwayRegulator of endocytic recyclingEndocytic recycling pathwayEndocytic recycling routeIntracellular recycling pathwayEndocytic cargoAntigen-presenting moleculesEndocytic traffickingEarly endosomesEndocytic recyclingGTPase Arl13bADP-ribosylationCilia formationCell surface moleculesArl13bInternal membranesSurface expressionCytoskeletonActinSurface moleculesPathwayRegulationArf6Forward genetics uncovers Transmembrane protein 107 as a novel factor required for ciliogenesis and Sonic hedgehog signaling
Christopher KJ, Wang B, Kong Y, Weatherbee SD. Forward genetics uncovers Transmembrane protein 107 as a novel factor required for ciliogenesis and Sonic hedgehog signaling. Developmental Biology 2012, 368: 382-392. PMID: 22698544, PMCID: PMC3402655, DOI: 10.1016/j.ydbio.2012.06.008.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBody PatterningCells, CulturedCiliaEmbryo, MammalianExtremitiesFemaleGene Expression Regulation, DevelopmentalHedgehog ProteinsIn Situ HybridizationKruppel-Like Transcription FactorsMaleMembrane ProteinsMiceMice, Inbred C3HMice, Inbred C57BLMice, Mutant StrainsMicroscopy, Electron, ScanningMolecular Sequence DataMutationNerve Tissue ProteinsNeural TubeSequence Homology, Amino AcidSignal TransductionZinc Finger Protein Gli2Zinc Finger Protein Gli3ConceptsCilia formationDistinct tissuesLeft-right patterning defectsForward genetic approachDevelopmental patterning eventsLeft-right specificationShh target genesNovel mutant allelesSonic hedgehog (Shh) signalingNeuronal cell typesEmbryonic patterningMutant phenotypeCilia mutantsDynamic organellesGli activatorPatterning defectsPatterning eventsRepressor formCilia biogenesisUnknown regulatorCilia formGenetic approachesSkeletal formationTarget genesTmem107
2011
Qilin Is Essential for Cilia Assembly and Normal Kidney Development in Zebrafish
Li J, Sun Z. Qilin Is Essential for Cilia Assembly and Normal Kidney Development in Zebrafish. PLOS ONE 2011, 6: e27365. PMID: 22102889, PMCID: PMC3216947, DOI: 10.1371/journal.pone.0027365.Peer-Reviewed Original ResearchConceptsCilia assemblyIFT complex B proteinsKidney developmentForward genetic screenCoiled-coil domainEssential roleKidney cystsNormal kidney developmentGenetic screenMutant phenotypeVestigial organelleNovel genesPolycystic kidney diseaseCilia formationDeletion analysisB geneB proteinB mutantsGenetic analysisMeckel-Gruber syndromeN-terminusFunctional analysisRescue experimentsZebrafishHuman diseasesChapter 3 Analysis of Cilia Structure and Function in Zebrafish
Malicki J, Avanesov A, Li J, Yuan S, Sun Z. Chapter 3 Analysis of Cilia Structure and Function in Zebrafish. Methods In Cell Biology 2011, 101: 39-74. PMID: 21550439, DOI: 10.1016/b978-0-12-387036-0.00003-7.Peer-Reviewed Original ResearchConceptsExcellent vertebrate model systemVertebrate model systemNormal embryonic developmentCell surface protrusionsCilia biologyGenetic accessibilityVertebrate cellsLimb morphogenesisCilia formationImportant organellesEmbryonic developmentLarval organsLeft-right asymmetryCilia structureDistribution of ciliaNephric ductZebrafishCiliary malfunctionVariety of processesPhotoreceptor cellsSensory cellsKidney cystsCiliaModel systemChapter 3 Analysis
2010
Intraflagellar Transport Proteins Are Essential for Cilia Formation and for Planar Cell Polarity
Cao Y, Park A, Sun Z. Intraflagellar Transport Proteins Are Essential for Cilia Formation and for Planar Cell Polarity. Journal Of The American Society Of Nephrology 2010, 21: 1326-1333. PMID: 20576807, PMCID: PMC2938599, DOI: 10.1681/asn.2009091001.Peer-Reviewed Original ResearchConceptsPlanar cell polarityIntraflagellar transport proteinsCell polarityCilia formationIFT mutantsIFT proteinsDefective planar cell polarityTransport proteinsCore PCP componentsKidney cyst formationConserved roleEarly developmental stagesCiliary assemblyIFT genesPCP componentsCilia formMulticiliated cellsPronephric ductGene productsMaternal contributionBasal bodiesMultiple organismsDevelopmental stagesMaternal depositionProteinThe immunological synapse: a focal point for endocytosis and exocytosis
Griffiths G, Tsun A, Stinchcombe J. The immunological synapse: a focal point for endocytosis and exocytosis. Journal Of Cell Biology 2010, 189: 399-406. PMID: 20439993, PMCID: PMC2867296, DOI: 10.1083/jcb.201002027.Peer-Reviewed Original ResearchRenal Cystic Proteins in the Olfactory Epithelium (OE)
Pluznick J, Rodriguez‐Gil D, Hull M, Mistry K, Gattone V, Johnson C, Weatherbee S, Greer C, Caplan M. Renal Cystic Proteins in the Olfactory Epithelium (OE). The FASEB Journal 2010, 24: 1002.17-1002.17. DOI: 10.1096/fasebj.24.1_supplement.1002.17.Peer-Reviewed Original ResearchMutant animalsPolycystin-2Olfactory epitheliumIndividual olfactory receptorsDisease-causing mutationsCilia formationΑ-tubulin stainingMurine olfactory epitheliumMature olfactory neuronsMKS3Olfactory receptorsOlfactory neuronsProteinDendritic knobsCiliaPhysiological activityMutant miceAC3 expressionVitro dataMutant ratsRT-PCRSyndrome 1Similar reductionExpressionRats
2009
Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion
Duldulao NA, Lee S, Sun Z. Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion. Development 2009, 136: 4033-4042. PMID: 19906870, PMCID: PMC2778746, DOI: 10.1242/dev.036350.Peer-Reviewed Original ResearchConceptsCilia formationVivo functionJoubert syndromeSeries of deletionsSonic hedgehog (Shh) signalingCilia localizationCiliary localizationNull mutantsPoint mutantsGene productsHedgehog signalingArl13bCiliary defectsMutantsKidney ductsZebrafishCiliaAutosomal recessive disorderAbnormal ultrastructureScorpionsRecessive disorderLocalizationRecent studiesKnockdownSignaling
2004
A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney
Sun Z, Amsterdam A, Pazour G, Cole D, Miller M, Hopkins N. A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney. Development 2004, 131: 4085-4093. PMID: 15269167, DOI: 10.1242/dev.01240.Peer-Reviewed Original ResearchConceptsIntraflagellar transportCystic kidney diseaseCilia formationOver-proliferation of epithelial cellsHuman cystic kidney diseasesPolycystic kidney diseaseInsertional mutagenesis screenKidney cystsChlamydomonas genesCiliary assemblyPKD genesCilia genesMutagenesis screenGenetic screeningEncode componentsPrimary ciliaGenesOver-proliferationCystic kidneysCiliaEpithelial cellsGenetic illnessMultiple kidney cystsZebrafishZebrafish larvae
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