2012
Forward genetics uncovers Transmembrane protein 107 as a novel factor required for ciliogenesis and Sonic hedgehog signaling
Christopher KJ, Wang B, Kong Y, Weatherbee SD. Forward genetics uncovers Transmembrane protein 107 as a novel factor required for ciliogenesis and Sonic hedgehog signaling. Developmental Biology 2012, 368: 382-392. PMID: 22698544, PMCID: PMC3402655, DOI: 10.1016/j.ydbio.2012.06.008.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBody PatterningCells, CulturedCiliaEmbryo, MammalianExtremitiesFemaleGene Expression Regulation, DevelopmentalHedgehog ProteinsIn Situ HybridizationKruppel-Like Transcription FactorsMaleMembrane ProteinsMiceMice, Inbred C3HMice, Inbred C57BLMice, Mutant StrainsMicroscopy, Electron, ScanningMolecular Sequence DataMutationNerve Tissue ProteinsNeural TubeSequence Homology, Amino AcidSignal TransductionZinc Finger Protein Gli2Zinc Finger Protein Gli3ConceptsCilia formationDistinct tissuesLeft-right patterning defectsForward genetic approachDevelopmental patterning eventsLeft-right specificationShh target genesNovel mutant allelesSonic hedgehog (Shh) signalingNeuronal cell typesEmbryonic patterningMutant phenotypeCilia mutantsDynamic organellesGli activatorPatterning defectsPatterning eventsRepressor formCilia biogenesisUnknown regulatorCilia formGenetic approachesSkeletal formationTarget genesTmem107Protection of hydroquinone-induced apoptosis by downregulation of Fau is mediated by NQO1
Siew E, Chan K, Williams G, Ross D, Inayat-Hussain S. Protection of hydroquinone-induced apoptosis by downregulation of Fau is mediated by NQO1. Free Radical Biology And Medicine 2012, 53: 1616-1624. PMID: 22687461, DOI: 10.1016/j.freeradbiomed.2012.05.046.Peer-Reviewed Original ResearchConceptsForward genetic approachDNA damageWild-type cellsDNA-damaging agentsPotential tumor suppressor geneSpecific mechanism-based inhibitorsTumor suppressor geneAntisense sequencesMouse thymoma cellsFAU geneGenetic approachesReactive oxygen speciesSuppressor geneDetoxification enzymesPlasmid constructsMechanism-based inhibitorsEmpty vectorApoptotic effectsQuinone oxidoreductase 1ApoptosisOxygen speciesThymoma cellsNQO1 upregulationGenesDownregulation
1999
A radiation hybrid map of the zebrafish genome
Geisler R, Rauch G, Baier H, van Bebber F, Broβ L, Dekens M, Finger K, Fricke C, Gates M, Geiger H, Geiger-Rudolph S, Gilmour D, Glaser S, Gnügge L, Habeck H, Hingst K, Holley S, Keenan J, Kirn A, Knaut H, Lashkari D, Maderspacher F, Martyn U, Neuhauss S, Neumann C, Nicolson T, Pelegri F, Ray R, Rick J, Roehl H, Roeser T, Schauerte H, Schier A, Schönberger U, Schönthaler H, Schulte-Merker S, Seydler C, Talbot W, Weiler C, Nüsslein-Volhard C, Haffter P. A radiation hybrid map of the zebrafish genome. Nature Genetics 1999, 23: 86-89. PMID: 10471505, DOI: 10.1038/12692.Peer-Reviewed Original ResearchConceptsSimple sequence length polymorphismsZebrafish genomeRadiation hybrid mapCandidate genesLarge-scale mutagenesis screensSomatic cell hybrid linesSequence tagged site (STS) markersHybrid mapForward genetic approachGenome-wide mapsTagged site (STS) markersSequence length polymorphismsDensity of markersSignificant linkageRodent cell linesVertebrate organismsGenetic mappingDevelopmental controlMutagenesis screenRadiation-induced breakpointsSpecies of interestPositional cloningRH panelGenetic approachesRetention frequency
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