2025
Acute Response of Hepatocyte MRP2 Internalization as an Indicator of Ischemia-reperfusion Injury in Liver Transplantation
Monti C, Hong S, Lee A, Hong J, Eriksen C, Joshi A, Dash R, Audi S, Lee W, Kumar S, Kim J. Acute Response of Hepatocyte MRP2 Internalization as an Indicator of Ischemia-reperfusion Injury in Liver Transplantation. Transplantation 2025, 109: 1495-1505. PMID: 40320583, DOI: 10.1097/tp.0000000000005418.Peer-Reviewed Original ResearchConceptsMultidrug resistance-associated protein 2Ischemia-reperfusion injuryNormothermic machine perfusionLiver transplantationIschemia timeGraft viabilityPerfusion levelsHepatic ischemia-reperfusion injurySerum aminotransferase levelsInverse correlationAcute responseLevels of liver injury markersLiver injury markersLiver graft viabilityImmunofluorescence colocalization analysisMrp2 internalizationAminotransferase levelsMRP2-mediated transport activityInjury markersRat modelHuman LTArginase 1Loss of functionBiliary excretionSodium fluorescein
2024
Assessing the degree of hepatic ischemia-reperfusion injury using physiologically based pharmacokinetic modeling of sodium fluorescein disposition in ex vivo machine-perfused livers
Monti C, Hong S, Audi S, Lee W, Joshi A, Terhune S, Kim J, Dash R. Assessing the degree of hepatic ischemia-reperfusion injury using physiologically based pharmacokinetic modeling of sodium fluorescein disposition in ex vivo machine-perfused livers. AJP Gastrointestinal And Liver Physiology 2024, 327: g424-g437. PMID: 38917324, PMCID: PMC11427087, DOI: 10.1152/ajpgi.00048.2024.Peer-Reviewed Original ResearchConceptsWarm ischemia timeIschemia-reperfusion injurySodium fluoresceinIschemia timeMachine perfusionIschemia-reperfusionMultidrug resistance-associated protein 2Prolonged warm ischemia timeBiliary excretionPharmacokinetic modelHepatic ischemia-reperfusion injuryHepatocyte membrane transportersEx vivo machine perfusionOrgan preservation techniquesLiver transplantationOne-way ANOVAHepatic transportersMeasurements of SFReal-time assessmentPerfusionProtein 2PBPK modelTime courseExcretionInjury
2023
Effects of tetrathiomolybdate on copper metabolism in healthy volunteers and in patients with Wilson disease
Kirk F, Munk D, Swenson E, Quicquaro A, Vendelbo M, Larsen A, Schilsky M, Ott P, Sandahl T. Effects of tetrathiomolybdate on copper metabolism in healthy volunteers and in patients with Wilson disease. Journal Of Hepatology 2023, 80: 586-595. PMID: 38081365, DOI: 10.1016/j.jhep.2023.11.023.Peer-Reviewed Original ResearchEffect of tetrathiomolybdateWilson's diseaseBiliary copper excretionBiliary excretionHealthy volunteersCopper excretionWD patientsBis-choline tetrathiomolybdateNeurologic Wilson diseaseClinical trial numberPresent human studyTrial numberPET/CTCopper metabolismIntestinal copper uptakeMechanism of actionPET/MRINeurological worseningConventional therapyVenous bloodClinical trialsLower riskAnimal studiesHuman studiesCopper chelatorTHU-309 Effects of tetrathiomolybdate on copper distribution and biliary excretion: a controlled 64CuCl2 PET/MRI
Kirk F, Munk D, Swenson E, Quicquaro A, Vendelbo M, Schilsky M, Ott P, Sandahl T. THU-309 Effects of tetrathiomolybdate on copper distribution and biliary excretion: a controlled 64CuCl2 PET/MRI. Journal Of Hepatology 2023, 78: s985. DOI: 10.1016/s0168-8278(23)03042-8.Peer-Reviewed Original ResearchPET/MRIBiliary excretion
2011
Wilson Disease
Schilsky M, Tavill A. Wilson Disease. 2011, 803-824. DOI: 10.1002/9781119950509.ch29.Peer-Reviewed Original ResearchWilson's diseaseExcellent patient survivalSevere hepatic insufficiencyAcute liver failureUrine copper excretionKayser-Fleischer ringsHepatic copper concentrationAsymptomatic patientsLiver transplantationPharmacologic treatmentSymptomatic patientsHepatic insufficiencyLiver failureMedical therapyPatient survivalDisease progressionBiliary excretionBiochemical findingsToxic copper accumulationCopper excretionPatientsDiseaseCopper-transporting adenosine triphosphataseDisease-specific mutationsGenetic disorders
2002
Mechanisms of Hepatic Transport of Drugs: Implications for Cholestatic Drug Reactions
Bohan A, Boyer J. Mechanisms of Hepatic Transport of Drugs: Implications for Cholestatic Drug Reactions. Seminars In Liver Disease 2002, 22: 123-136. PMID: 12016544, DOI: 10.1055/s-2002-30099.Peer-Reviewed Original ResearchConceptsDrug-induced cholestasisCholestatic drug reactionDrug reactionsDrug transporter expressionNumber of drugsBiliary excretionCanalicular transport proteinsAnimal modelsHepatic uptakeHepatobiliary transportersCholestasisTransporter expressionHepatic transportGenetic polymorphismsNormal functionDrugsMajor determinantForeign compoundsStudy of mechanismsTransportersExcretion
1999
Hepatic sequestration and modulation of the canalicular transport of the organic cation, daunorubicin, in the rat
Hayes J, Soroka C, Rios‐Velez L, Boyer J. Hepatic sequestration and modulation of the canalicular transport of the organic cation, daunorubicin, in the rat. Hepatology 1999, 29: 483-493. PMID: 9918926, DOI: 10.1002/hep.510290216.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntineoplastic AgentsATP Binding Cassette Transporter, Subfamily B, Member 1BileBile CanaliculiBiological TransportBucladesineCationsChloroquineDaunorubicinKineticsLiverMacrolidesMaleMitochondrial ProteinsNocodazoleRatsRats, WistarRibosomal ProteinsSaccharomyces cerevisiae ProteinsTaurocholic AcidVerapamilConceptsBiliary excretionCoadministration of verapamilInhibitors of MDR1Rat hepatocyte coupletsBiliary recoveryDose administeredHepatic sequestrationTR- mutant ratsTherapeutic agentsExcretionDaunorubicinPericanalicular regionHepatocyte coupletsCanalicular transportRat liverBafilomycin ATaurocholateRatsIPRLIntracellular acidic compartmentsCellular localizationMicrotubule destabilizerConfocal microscopyIRHCPattern of distribution
1998
Role of glutathione in hepatic bile formation during reperfusion after cold ischemia of the rat liver
Koeppel T, Trauner M, Albert M, Arrese M, Rios-Velez L, Boyer J. Role of glutathione in hepatic bile formation during reperfusion after cold ischemia of the rat liver. Journal Of Hepatology 1998, 28: 812-819. PMID: 9625316, DOI: 10.1016/s0168-8278(98)80231-6.Peer-Reviewed Original ResearchConceptsBiliary glutathione excretionBile duct injuryCold ischemiaBile flowHepatic bile formationDuct injuryLiver transplantationBiliary tractBiliary excretionRat liverGlutathione excretionBasal bile flow ratesBile formationBile acid-independent bile flowOxidative stressLate reperfusion periodBile duct damageBile flow rateOnset of reperfusionIndependent bile flowBile duct morphologyBiliary glutathione concentrationsWistar rat liverLiver reperfusionDuct damage
1993
Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL
Bruck R, Benedetti A, Strazzabosco M, Boyer J. Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL. American Journal Of Physiology 1993, 265: g347-g353. PMID: 8368316, DOI: 10.1152/ajpgi.1993.265.2.g347.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAlkaliesAnimalsAnionsBariumBicarbonatesBileBiological TransportColchicineExocytosisHorseradish PeroxidaseHydrogen-Ion ConcentrationIn Vitro TechniquesIntracellular MembranesLiverMalePotassium ChannelsRatsRats, Sprague-DawleyConceptsBiliary HCO3- excretionBile flowHCO3- excretionIntracellular alkalinizationHCO3- exchangerAcute alkaline loadVesicle-mediated exocytosisBiliary excretionTransient increasePH recoveryAlkaline loadMembrane depolarizationRat liverChannel blocker BaCl2Intact liverLiverAlkalinizationMM NH4ClHCO3- concentrationDisulfonic acidDIDSRat hepatocytesColchicinePretreatmentExocytosis
1992
Papaverine inhibits transcytotic vesicle transport and lipid excretion into bile in isolated perfused rat liver
Hayakawa T, Katagiri K, Hoshino M, Nakai T, Ohiwa T, Kumai T, Miyaji M, Takeuchi T, Corasanti J, Boyer J. Papaverine inhibits transcytotic vesicle transport and lipid excretion into bile in isolated perfused rat liver. Hepatology 1992, 16: 1036-1042. PMID: 1398483, DOI: 10.1002/hep.1840160429.Peer-Reviewed Original ResearchConceptsSodium taurocholate infusionBiliary excretionTaurocholate infusionPhosphodiesterase inhibitorBile acidsNonspecific smooth muscle relaxantSmooth muscle relaxantRat liver modelRat liverLipid excretionContinuous infusionBile flowMuscle relaxantsPapaverine inhibitsConstant infusionHorseradish peroxidaseInfusionPapaverineExcretionInhibitory effectCyclic GMPBileLiverLiver modelCyclic AMPRegulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver
Bruck R, Haddad P, Graf J, Boyer J. Regulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver. American Journal Of Physiology 1992, 262: g806-g812. PMID: 1590390, DOI: 10.1152/ajpgi.1992.262.5.g806.Peer-Reviewed Original Research
1990
DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver
Hayakawa T, Bruck R, Ng O, Boyer J. DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver. American Journal Of Physiology 1990, 259: g727-g735. PMID: 2173414, DOI: 10.1152/ajpgi.1990.259.5.g727.Peer-Reviewed Original ResearchConceptsBiliary excretionBile acidsBile acid excretionLiver 1Effects of adenosineRat liverElectron microscopic morphometric analysisTranscytotic vesicle pathwayLate peakHRP excretionHorseradish peroxidaseMin infusionBile flowExcretory functionAcid excretionO-dibutyryl cAMPPrimary hepatocyte culturesExcretionLiverDbcAMPCyclic monophosphateInfusionPericanalicular area
1988
Biliary catabolism of glutathione and differential reabsorption of its amino acid constituents
Ballatori N, Jacob R, Barrett C, Boyer J. Biliary catabolism of glutathione and differential reabsorption of its amino acid constituents. American Journal Of Physiology 1988, 254: g1-g7. PMID: 2892423, DOI: 10.1152/ajpgi.1988.254.1.g1.Peer-Reviewed Original ResearchConceptsGuinea pigsBile samplesAT-125Biliary glutathione excretionControl bile samplesHepatic gamma-glutamyltransferase activityCatabolism of glutathioneTotal amino acidsGamma-glutamyltransferase activityBiliary treePentobarbital sodiumBiliary excretionHepatic bileAmino acid conjugationGlutathione excretionRetrograde intrabiliary infusionBileConcentration of glutathioneFree amino acidsTotal glutamateConstituent amino acidsMarked increaseUndetectable levelsAcid conjugationDogs
1987
The excretion pattern of biliverdin and bilirubin in bile of the small skate (Raja erinacea)
Grossbard M, Boyer J, Gordon E. The excretion pattern of biliverdin and bilirubin in bile of the small skate (Raja erinacea). Journal Of Comparative Physiology B 1987, 157: 61-66. PMID: 3571566, DOI: 10.1007/bf00702729.Peer-Reviewed Original ResearchConceptsIntravenous administrationHepatic bileGallbladder volumeBile pigment compositionBiliary excretionGallbladder bileExcretion patternsBilirubin conjugatesUnconjugated bilirubinBileBilirubinBilirubin diglucuronideBilirubin monoglucuronideSignificant increaseAdministrationSmall skateBiliverdinDay period
1986
Demonstration of a Transcellular Vesicle Pathway for Biliary Excretion of Inulin in Rat Liver
Lorenzini I, Sakisaka S, Meier P, Boyer J. Demonstration of a Transcellular Vesicle Pathway for Biliary Excretion of Inulin in Rat Liver. Gastroenterology 1986, 91: 1278-1288. PMID: 3758619, DOI: 10.1016/s0016-5085(86)80028-2.Peer-Reviewed Original ResearchConceptsTaurochenodeoxycholic acidBiliary excretionTotal bile productionVesicle-mediated transcytosisRenal pedicleBile productionBody wtInulin clearanceIntravenous injectionBasal conditionsLiver homogenatesRatsHepatocellular uptakeExcretionRat liverTranscellular transportTranscellular pathwayBileSubcellular fractionation studiesBloodSimilar effectsVesicle pathwayHorseradish peroxidaseIntrabiliary glutathione hydrolysis. A source of glutamate in bile.
Ballatori N, Jacob R, Boyer J. Intrabiliary glutathione hydrolysis. A source of glutamate in bile. Journal Of Biological Chemistry 1986, 261: 7860-7865. PMID: 2872220, DOI: 10.1016/s0021-9258(19)57482-8.Peer-Reviewed Original ResearchConceptsGamma-glutamyl transferase activityBiliary excretionRat bileRetrograde intrabiliary infusionAT-125Normal male Sprague-DawleyAdministration of phenolMale Sprague-DawleyNormal rat bileDihydro-5-isoxazoleacetic acidSource of glutamateIntrabiliary infusionRate of excretionDibromphthalein disulfonateBranched chain amino acids valineBiliary treeSprague-DawleyDiethyl maleateBileExcretionTransferase activityGSH secretionLiver cellsMarked decreaseExcretion of glutamate
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