2018
MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis
Rahman NT, Schulz VP, Wang L, Gallagher PG, Denisenko O, Gualdrini F, Esnault C, Krause DS. MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis. Blood Advances 2018, 2: 2691-2703. PMID: 30337297, PMCID: PMC6199649, DOI: 10.1182/bloodadvances.2018019448.Peer-Reviewed Original ResearchConceptsSerum response factorHEL cellsTarget genesBinding of SRFMegakaryocyte maturationActivity of SRFSRF target genesUpregulated target genesMyocardin family proteinsTernary complex factor familyTransformation-specific proteinsPrimary hematopoietic cellsHuman erythroleukemia cell lineErythroleukemia cell lineCArG sitesPrimary human CD34Genomic sitesGenomic regionsChromatin immunoprecipitationETS factorsTranscription factorsHuman megakaryopoiesisGenomic associationsMRTFAFactor family
2012
Altered subcellular localization of transcription factor TEAD4 regulates first mammalian cell lineage commitment
Home P, Saha B, Ray S, Dutta D, Gunewardena S, Yoo B, Pal A, Vivian JL, Larson M, Petroff M, Gallagher PG, Schulz VP, White KL, Golos TG, Behr B, Paul S. Altered subcellular localization of transcription factor TEAD4 regulates first mammalian cell lineage commitment. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 7362-7367. PMID: 22529382, PMCID: PMC3358889, DOI: 10.1073/pnas.1201595109.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlastocystBlastocyst Inner Cell MassBlastomeresBlotting, WesternCattleCDX2 Transcription FactorCell LineageCell NucleusCells, CulturedDNA-Binding ProteinsEmbryonic Stem CellsGATA3 Transcription FactorGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsHEK293 CellsHomeodomain ProteinsHumansMacaca mulattaMiceMice, TransgenicMuscle ProteinsRatsReverse Transcriptase Polymerase Chain ReactionRNA InterferenceTEA Domain Transcription FactorsTranscription FactorsConceptsInner cell massTranscriptional programsICM lineagesSubcellular localizationNuclear localizationInner blastomeresCell fate specificationSpecific transcriptional programsCell lineage commitmentAltered subcellular localizationTranscription factor TEAD4Preimplantation mouse embryosFate specificationLineage commitmentTarget genesMouse embryosCell lineagesTEAD4LineagesBlastomeresBlastocyst formationCell massDifferential functionGenesLocalization
2009
Genome-Wide ChIP-Seq Reveals a Dramatic Shift in the EKLF Binding Profile Between Erythroid Progenitors and Erythroblasts.
Pilon A, Ajay S, Abaan H, Margulies E, Gallagher P, Bodine D. Genome-Wide ChIP-Seq Reveals a Dramatic Shift in the EKLF Binding Profile Between Erythroid Progenitors and Erythroblasts. Blood 2009, 114: 565. DOI: 10.1182/blood.v114.22.565.565.Peer-Reviewed Original ResearchTarget genesChIP-seqNearest geneSimultaneous genome-wide analysisC2H2 zinc finger transcription factorGenome-wide ChIP-seqErythroid Kruppel-like factorZinc finger transcription factorErythroid progenitorsTranscription factor occupancyBeta-globin locusGenome-wide analysisCell cycle control factorsProtein-DNA interactionsFinger transcription factorChIP-seq dataS-phase entryNon-repetitive regionsKruppel-like factorEKLF geneEKLF proteinFetal liverCell cycle S-phase entryChromatin remodelingFactor occupancy
2008
Failure of Terminal Erythroid Differentiation in EKLF-Deficient Mice Is Associated with Cell Cycle Perturbation and Reduced Expression of E2F2
Pilon AM, Arcasoy MO, Dressman HK, Vayda SE, Maksimova YD, Sangerman JI, Gallagher PG, Bodine DM. Failure of Terminal Erythroid Differentiation in EKLF-Deficient Mice Is Associated with Cell Cycle Perturbation and Reduced Expression of E2F2. Molecular And Cellular Biology 2008, 28: 7394-7401. PMID: 18852285, PMCID: PMC2593440, DOI: 10.1128/mcb.01087-08.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell DifferentiationE2F2 Transcription FactorEmbryo, MammalianErythropoiesisGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Regulatory NetworksKruppel-Like Transcription FactorsLiverMiceMice, KnockoutOligonucleotide Array Sequence AnalysisPromoter Regions, GeneticStem CellsTranscription, GeneticConceptsErythroid Krüppel-like factorTerminal erythroid differentiationEarly erythroid progenitor cellsErythroid progenitor cellsErythroid differentiationChromatin modifiersProgenitor cellsKrüppel-like transcription factorsNetwork of genesCell cycle regulationChromatin immunoprecipitation analysisKrüppel-like factorCell cycle progressionFailure of erythropoiesisS phase transitionEarly progenitor cellsTranscriptional activatorCycle regulationTranscriptional profilingTranscription factorsTarget genesImmunoprecipitation analysisDNase IErythroid cellsCycle progressionGenome-Wide Analysis of EKLF Occupancy in Erythroid Chromatin Reveals 5′, 3′ and Intragenic Binding Sites in EKLF Target Genes
Pilon A, Margulies E, Abaan H, Allen A, Townes T, Frederick A, Zhou D, Gallagher P, Bodine D. Genome-Wide Analysis of EKLF Occupancy in Erythroid Chromatin Reveals 5′, 3′ and Intragenic Binding Sites in EKLF Target Genes. Blood 2008, 112: 283. DOI: 10.1182/blood.v112.11.283.283.Peer-Reviewed Original ResearchCell cycle networkChIP-seqIngenuity Pathway AnalysisTranscription factorsEKLF geneWide analysisIntragenic sitesTarget genesChIP-PCRErythroid cellsMapping protein-DNA interactionsErythroid Kruppel-like factorFetal liver erythroid cellsGenome-wide scaleBeta-globin locusGenome-wide analysisCell cycle control networkProtein-DNA interactionsChIP-seq resultsTerminal erythroid maturationDNase hypersensitive sitesKnowledge of genesChIP-seq librariesChIP-seq dataFL cells
2006
Defects in E2F1/2 Expression Are Associated with Abnormalities in Cell Cycle and Differentiation in EKLF-Deficient Erythroid Cells.
Pilon A, Arcasoy M, Vayda S, Dressman H, Bieker J, Bodine D, Gallagher P. Defects in E2F1/2 Expression Are Associated with Abnormalities in Cell Cycle and Differentiation in EKLF-Deficient Erythroid Cells. Blood 2006, 108: 84. DOI: 10.1182/blood.v108.11.84.84.Peer-Reviewed Original ResearchHypersensitive sitesErythroid cellsMicroarray analysisCell cycleNumerous genesMature erythroblastsGel mobility shift assaysMicroarray dataFL cellsTranscription factor EKLFCell cycle controlMobility shift assaysΒ-globin expressionIngenuity Pathway AnalysisImmature erythroid cellsPrevious microarray analysisPrevious microarray dataE2F proteinsDefinitive erythropoiesisDNA replicationTranscription factorsColony forming assaysEKLFShift assaysTarget genes
2004
Multiple Defects in Erythroid Gene Expression in Erythroid Krupple-Like Factor (EKLF) Target Genes in EKLF-Deficient Mice.
Gallagher P, Pilon A, Arcasoy M, Bodine D. Multiple Defects in Erythroid Gene Expression in Erythroid Krupple-Like Factor (EKLF) Target Genes in EKLF-Deficient Mice. Blood 2004, 104: 1602. DOI: 10.1182/blood.v104.11.1602.1602.Peer-Reviewed Original ResearchDNaseI hypersensitive sitesSubtractive hybridizationHypersensitive sitesΒ-globin geneHistone H3Transcription factorsTarget genesFetal liver cellsΒ-spectrinGene expressionΒ-globinErythroid gene expressionMouse Genome 430 2.0 ArrayAffymetrix GeneChip Mouse Genome 430 2.0 ArrayFactor target genesAHSP gene expressionChromatin immunoprecipitation analysisBand 3Mature erythroid progenitorsAHSP promoterRBC membrane proteinsErythroid genesChromatin upstreamChromatin modulatorsRegulated genes