2024
HEXIM1 Regulates Early Erythropoiesis and Participates in Multiple Complexes in Erythroid Cells
Rahman N, Abid D, Lv X, Murphy K, Getman M, McGrath K, Gallagher P, Narla M, Blanc L, Palis J, Mello S, Steiner L. HEXIM1 Regulates Early Erythropoiesis and Participates in Multiple Complexes in Erythroid Cells. Blood 2024, 144: 536. DOI: 10.1182/blood-2024-209259.Peer-Reviewed Original ResearchRNA polymerase IIErythroid gene expressionGene expressionTerminal erythroid maturationEarly erythropoiesisErythroid cellsErythroid maturationRegulation of gene expressionProgenitor cellsImpaired erythroid differentiationRNAPII pausingGenomic contextPolymerase IIRepress transcriptionSteady-state erythropoiesisErythroid progenitor cellsCD34+ HSPCsRegulatory domainBinding partnersErythropoiesis in vivoBlood cell countColony-forming cellsLow red blood cell countSubnuclear bodiesErythroid progenitor differentiation
2023
Phenotypic and proteomic characterization of the human erythroid progenitor continuum reveal dynamic changes in cell cycle and in metabolic pathways
Papoin J, Yan H, Leduc M, Le Gall M, Narla A, Palis J, Steiner L, Gallagher P, Hillyer C, Gautier E, Mohandas N, Blanc L. Phenotypic and proteomic characterization of the human erythroid progenitor continuum reveal dynamic changes in cell cycle and in metabolic pathways. American Journal Of Hematology 2023, 99: 99-112. PMID: 37929634, PMCID: PMC10877306, DOI: 10.1002/ajh.27145.Peer-Reviewed Original ResearchConceptsErythroid progenitor differentiationCell cycleErythroid progenitorsProgenitor differentiationMass spectrometry-based proteomicsFurther functional analysisSpectrometry-based proteomicsHuman erythroid progenitorsProtein machineryErythroid progenitor proliferationTerminal erythropoiesisProteomic characterizationHematopoietic stem cellsProteomic dataProgenitor populationsHuman erythropoiesisReticulocyte maturationFunctional analysisErythroid lineageOxidative phosphorylationProgenitor proliferationErythroid disordersMetabolic pathwaysAbsolute expressionStem cellsPhenotypic and Proteomic Characterization of the Human Erythroid Progenitor Continuum Reveal Dynamic Changes in Cell Cycle and in Metabolic Pathways
Papoin J, Yan H, Leduc M, le-Gall M, Narla A, Steiner L, Gallagher P, Hillyer C, Gauthier E, Narla M, Blanc L. Phenotypic and Proteomic Characterization of the Human Erythroid Progenitor Continuum Reveal Dynamic Changes in Cell Cycle and in Metabolic Pathways. Blood 2023, 142: 2455. DOI: 10.1182/blood-2023-189566.Peer-Reviewed Original ResearchCell cycleFaster cycling cellsProgenitor differentiationProgenitor populationsOxidative phosphorylationMass spectrometry-based proteomicsErythroid progenitorsS phaseProtein copy numbersSpectrometry-based proteomicsErythroid progenitor differentiationCycle-related genesG2/MTerminal erythropoiesisProgenitor biologyProteomic characterizationHematopoietic stem cellsE2F membersHuman erythropoiesisHuman bone marrowFunctional analysisErythroid lineageMurine erythropoiesisMetabolic pathwaysS transition