2007
Alternate Promoters Direct Tissue-Specific Expression of Erythrocyte Ankyrin Transcripts with Novel NH2-Termini.
Steiner L, Lin J, Owens A, Sangerman J, Bodine D, Gallagher P. Alternate Promoters Direct Tissue-Specific Expression of Erythrocyte Ankyrin Transcripts with Novel NH2-Termini. Blood 2007, 110: 1710. DOI: 10.1182/blood.v110.11.1710.1710.Peer-Reviewed Original ResearchExon 1AExon 1bInitiator methionineFirst exonKb downstreamErythroid cellsNH2 terminusDNase I hypersensitive site mappingDirect tissue-specific expressionAmino acidsHypersensitive site mappingTissue-restricted patternTissue-specific expressionExon 1Alternate first exonsQuantitative RT-PCR analysisGATA-1 sitesTissue-specific promotersGene expression studiesLuciferase expressionExon 2 sequencesSkeletal muscleIsoform diversityAnkyrin repeatsAnkyrin-1 mutations
2005
The Human Alpha Hemoglobin Stabilizing Protein (AHSP) Gene Locus in EKLF-Deficient Erythroid Cells.
Pilon A, Zhou D, Weiss M, Townes T, Bodine D, Gallagher P. The Human Alpha Hemoglobin Stabilizing Protein (AHSP) Gene Locus in EKLF-Deficient Erythroid Cells. Blood 2005, 106: 1740. DOI: 10.1182/blood.v106.11.1740.1740.Peer-Reviewed Original ResearchCACCC siteErythroid cellsWild typeModifier genesDNase I hypersensitive site mappingLocal chromatin structureΒ-globin transcriptionErythroid-specific proteinΒ-globin gene promoterDNA-protein complexesFetal liver cellsTranscription factor EKLFChromatin immunoprecipitation analysisΒ-globin sequencesHypersensitive site mappingFetal liverMobility shift assaysPromoter-luciferase reporter plasmidProximal CACCC boxFetal liver DNACore promoter regionAHSP promoterDeficient fetal liver cellsEKLF geneChromatin modulators