2021
Regulation of RNA polymerase II activity is essential for terminal erythroid maturation
Murphy ZC, Murphy K, Myers J, Getman M, Couch T, Schulz VP, Lezon-Geyda K, Palumbo C, Yan H, Mohandas N, Gallagher PG, Steiner LA. Regulation of RNA polymerase II activity is essential for terminal erythroid maturation. Blood 2021, 138: 1740-1756. PMID: 34075391, PMCID: PMC8569412, DOI: 10.1182/blood.2020009903.Peer-Reviewed Original ResearchConceptsRNA polymerase IIRNA polymerase II activityTerminal erythroid maturationPolymerase II activityPolymerase IIErythroid maturationHuman erythroblastsGene expressionTerminal maturationII activityStage-specific regulationHistone posttranslational modificationsTransposase-accessible chromatinErythroid-specific genesAccumulation of heterochromatinHigh-throughput sequencingLevel of transcriptionLate-stage erythroblastsEssential biologic processesAccessible chromatinHistone marksTranscription elongationChromatin structureTranscriptional repressionChromatin immunoprecipitation
2016
Setd1a and NURF mediate chromatin dynamics and gene regulation during erythroid lineage commitment and differentiation
Li Y, Schulz VP, Deng C, Li G, Shen Y, Tusi BK, Ma G, Stees J, Qiu Y, Steiner LA, Zhou L, Zhao K, Bungert J, Gallagher PG, Huang S. Setd1a and NURF mediate chromatin dynamics and gene regulation during erythroid lineage commitment and differentiation. Nucleic Acids Research 2016, 44: 7173-7188. PMID: 27141965, PMCID: PMC5009724, DOI: 10.1093/nar/gkw327.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, NuclearCell LineageCells, CulturedChromatinChromatin Assembly and DisassemblyChromatin ImmunoprecipitationErythroblastsErythrocyte CountErythrocytesErythropoiesisFemaleGene Expression RegulationHemoglobinsHistone-Lysine N-MethyltransferaseHistonesHumansLysineMaleMethylationMiceMice, KnockoutMicrococcal NucleaseMultiprotein ComplexesNerve Tissue ProteinsPromoter Regions, GeneticSpleenTranscription FactorsUpstream Stimulatory FactorsConceptsNURF complexChromatin dynamicsErythroid genesLineage commitmentAdult β-globin geneErythroid gene promotersErythroid lineage differentiationCell context-dependent mannerErythroid lineage commitmentChromatin structural alterationsContext-dependent mannerΒ-globin geneChromatin architectureEnhancer accessibilityChromatin accessibilityNucleosome repositioningTranscription regulationChromatin structureH3K4 methylationGene regulationComplex occupancyMammalian cellsGene activationGene transcriptionLineage differentiation
2011
Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes
Li X, Wang S, Li Y, Deng C, Steiner LA, Xiao H, Wu C, Bungert J, Gallagher PG, Felsenfeld G, Qiu Y, Huang S. Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes. Blood 2011, 118: 1386-1394. PMID: 21653943, PMCID: PMC3152501, DOI: 10.1182/blood-2010-11-319111.Peer-Reviewed Original ResearchConceptsErythroid genesInsulator sitesBarrier activityActive chromatin structureNucleosome remodeling activitiesChromatin barrier activityHistone H3K4 methyltransferaseChicken β-globinChromatin boundariesNURF complexChromatin structureInsulator functionNucleosome positioningMultiprotein complexesProtein complexesH3K4 methyltransferaseHS4 insulatorChromatin insulatorNURFH3K27me3 levelsLoci resultsLinker regionKnock-downΒ-globinFunctional collaboration
2006
Alterations in Expression and Chromatin Configuration of the Alpha Hemoglobin-Stabilizing Protein Gene in Erythroid Krüppel-Like Factor-Deficient Mice
Pilon AM, Nilson DG, Zhou D, Sangerman J, Townes TM, Bodine DM, Gallagher PG. Alterations in Expression and Chromatin Configuration of the Alpha Hemoglobin-Stabilizing Protein Gene in Erythroid Krüppel-Like Factor-Deficient Mice. Molecular And Cellular Biology 2006, 26: 4368-4377. PMID: 16705186, PMCID: PMC1489081, DOI: 10.1128/mcb.02216-05.Peer-Reviewed Original ResearchConceptsErythroid Krüppel-like factorAlpha-hemoglobin-stabilizing proteinWild-type chromatinAHSP promoterCACCC siteBeta-globin gene transcriptionDNase I hypersensitive sitesLocal chromatin structureZinc finger proteinBeta-globin promoterKrüppel-like factorMobility shift assaysBeta-globin geneErythroid genesCACCC sequenceChromatin modulatorsFinger proteinChromatin structureSubtractive hybridizationChromatin statusCACCC boxTranscription factorsProtein geneChromatin configurationHypersensitive sites
2005
Genomic Organization, Chromatin Structure, and Transcriptional Regulation of the Murine Alpha Hemoglobin Stabilizing Protein (AHSP) Gene.
Dore L, Vakoc C, Blobel G, Hardison R, Bodine D, Gallagher P, Weiss M. Genomic Organization, Chromatin Structure, and Transcriptional Regulation of the Murine Alpha Hemoglobin Stabilizing Protein (AHSP) Gene. Blood 2005, 106: 3633. DOI: 10.1182/blood.v106.11.3633.3633.Peer-Reviewed Original ResearchGATA-1AHSP geneChromatin structureHistone H3 lysine 4Alpha-Hemoglobin Stabilizing ProteinDNase I hypersensitive sitesGene regulatory domainsH3 lysine 4Kb genomic regionTerminal erythroid maturationGATA-1 bindingErythroid-specific expressionTransient transfection assaysInitial functional studiesAHSP promoterSyntenic blocksCandidate modifier genesGenomic organizationLysine 4Erythroid proteinGene regulationStabilizing ProteinTranscriptional regulationAdjacent genesGenomic regionsChromatin Conformation and a Distal Regulatory Element Activate the Human Erythroid Ankyrin-1 Promoter.
Owen A, Liem R, Pilon A, Gallagher P, Bodine D. Chromatin Conformation and a Distal Regulatory Element Activate the Human Erythroid Ankyrin-1 Promoter. Blood 2005, 106: 803. DOI: 10.1182/blood.v106.11.803.803.Peer-Reviewed Original ResearchNon-erythroid cellsRegulatory elementsReporter geneErythroid cellsKb regionLuciferase reporter geneDNase IKb downstreamKb upstreamK562 cellsErythroid-specific transcription factorDNase I hypersensitive sitesE-box binding proteinGreen fluorescent protein (GFP) reporter geneCell typesChromatin conformation captureFluorescent protein reporter geneGFP reporter genePositive regulatory elementErythrocyte membrane skeletonConformation captureChromatin conformationChromatin loopsChromatin structureTranscription factors