2010
Functional Analysis of a Novel cis-Acting Regulatory Region within the Human Ankyrin Gene (ANK-1) Promoter
Laflamme K, Owen AN, Devlin EE, Yang MQ, Wong C, Steiner LA, Garrett LJ, Elnitski L, Gallagher PG, Bodine DM. Functional Analysis of a Novel cis-Acting Regulatory Region within the Human Ankyrin Gene (ANK-1) Promoter. Molecular And Cellular Biology 2010, 30: 3493-3502. PMID: 20479128, PMCID: PMC2897556, DOI: 10.1128/mcb.00119-10.Peer-Reviewed Original ResearchMeSH Keywords5' Untranslated RegionsAnimalsAnkyrinsBase SequenceBinding SitesCell-Free SystemConsensus SequenceDNADNA PrimersGene LibraryHumansIn Vitro TechniquesMiceMice, TransgenicMolecular Sequence DataPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidSequence DeletionSequence Homology, Nucleic AcidTATA-Box Binding ProteinTranscription Factor TFIIDTranscription, GeneticConceptsCell-free transcriptionPromoter functionGene promoterDinucleotide deletionANK-1 geneNovel regulatory elementFunctional promoter sequencesNovel functional motifsTransgenic mouse assaysPreinitiation complexRegulatory sequencesPromoter sequencesRegulatory regionsRegulatory elementsFunctional motifsUntranslated regionWild typeFunctional analysisAdditional sequencesDegenerate sequencePromoterMutationsTranscriptionDeletionSequence
2008
Local and Distant Elements Regulate Tissue-Specific Expression of ANK-1 Gene Transcripts
Owen A, Laflamme K, Pilon A, Garrett L, Gallagher P, Bodine D. Local and Distant Elements Regulate Tissue-Specific Expression of ANK-1 Gene Transcripts. Blood 2008, 112: 2432. DOI: 10.1182/blood.v112.11.2432.2432.Peer-Reviewed Original ResearchNon-erythroid cellsHypersensitive sitesTransient transfection assaysConsensus sequenceChromatin loopsGATA-1NF-E2Promoter sequencesKb regionHistone acetylationErythroid transcription factor NF-E2Transfection assaysFirst exonDNase IErythroid cellsDevelopmental stage-specific patternsLocal chromatin architectureProtein-coding genesChromatin conformation captureANK-1 geneRNA Pol IITranscription initiation complexErythroid K562 cellsDNase hypersensitive sitesGATA-1 binding
2006
Identification of a Novel Core Promoter Element That Enhances Transcription: Application to Ankyrin Promoters for Globin Gene Therapy.
Laflamme K, Elnitski L, Owen A, Gallagher P, Bodine D. Identification of a Novel Core Promoter Element That Enhances Transcription: Application to Ankyrin Promoters for Globin Gene Therapy. Blood 2006, 108: 3261. DOI: 10.1182/blood.v108.11.3261.3261.Peer-Reviewed Original ResearchGC-rich promoterLocus control regionΓ-globin expressionHematopoietic stem cellsTG dinucleotideErythroid cellsNovel core promoter elementΑ-globinDifferent tissue-specific promotersLike globin genesBinding regionsΓ-globin transcriptionANK-1 geneTranscription initiation complexΓ-globin geneErythroid K562 cellsConsensus promoter sequenceTranscriptional start siteCore promoter elementsRNA virus vectorMouse erythroid cellsK562 cellsStable transfection assaysWild-type promoterTissue-specific promoters
2000
A Minimal Ankyrin Promoter Linked to a Human γ-Globin Gene Demonstrates Erythroid Specific Copy Number Dependent Expression with Minimal Position or Enhancer Dependence in Transgenic Mice*
Sabatino D, Wong C, Cline A, Pyle L, Garrett L, Gallagher P, Bodine D. A Minimal Ankyrin Promoter Linked to a Human γ-Globin Gene Demonstrates Erythroid Specific Copy Number Dependent Expression with Minimal Position or Enhancer Dependence in Transgenic Mice*. Journal Of Biological Chemistry 2000, 275: 28549-28554. PMID: 10878017, DOI: 10.1074/jbc.m004043200.Peer-Reviewed Original ResearchConceptsAnkyrin promoterPosition-independent expressionErythroid cellsGlobin genesGlobin mRNAHuman γ-globin genesMouse alpha-globin mRNABeta-globin locus control regionAdult erythroid cellsANK-1 geneΓ-globin geneCopy number-dependent expressionLocus control regionErythroid-specific expressionAlpha-globin mRNAFetal erythroid cellsNumber-dependent expressionGlobin mRNA expressionErythrocyte membrane skeletonTransgene copy numberHS2 enhancerTransgenic linesGlobin transgenesControl regionTransgenic mice
1997
Structure and Organization of the Human Ankyrin-1 Gene BASIS FOR COMPLEXITY OF PRE-mRNA PROCESSING*
Gallagher P, Tse W, Scarpa A, Lux S, Forget B. Structure and Organization of the Human Ankyrin-1 Gene BASIS FOR COMPLEXITY OF PRE-mRNA PROCESSING*. Journal Of Biological Chemistry 1997, 272: 19220-19228. PMID: 9235914, DOI: 10.1074/jbc.272.31.19220.Peer-Reviewed Original ResearchConceptsGenomic structureANK-1 geneSpectrin-binding domainMembrane-binding domainIntron/exon boundariesAlternative polyadenylation signalsPre-mRNA processingBrain-specific transcriptFurther genetic studiesErythrocyte membrane proteinsErythroid transcriptsCommon hemolytic anemiaGene basisAlternative splicingRegulatory domainProtein domainsChromosomal genesMembrane proteinsKilobase pairsPolyadenylation signalMembrane skeletonPlasma membraneExon boundariesGenetic studiesAnkyrin 1