Mario Skarica, MD
Associate Research ScientistCards
About
Research
Publications
2023
Single Cell Genomic Analysis Reveals Cell Type-Specific Molecular Signatures in the Human PTSD Prefrontal Cortex
Girgenti M, Zhang J, Skarica M, Hwang A, Xu K, Young K, Zhao H, Sestan N, Krystal J. Single Cell Genomic Analysis Reveals Cell Type-Specific Molecular Signatures in the Human PTSD Prefrontal Cortex. Biological Psychiatry 2023, 93: s11-s12. DOI: 10.1016/j.biopsych.2023.02.049.Peer-Reviewed Original Research
2022
AgRP neurons control structure and function of the medial prefrontal cortex
Stutz B, Waterson MJ, Šestan-Peša M, Dietrich MO, Škarica M, Sestan N, Racz B, Magyar A, Sotonyi P, Liu ZW, Gao XB, Matyas F, Stoiljkovic M, Horvath TL. AgRP neurons control structure and function of the medial prefrontal cortex. Molecular Psychiatry 2022, 27: 3951-3960. PMID: 35906488, PMCID: PMC9891653, DOI: 10.1038/s41380-022-01691-8.Peer-Reviewed Original ResearchConceptsMedial prefrontal cortexAgRP neuronsNon-selective dopamine receptor antagonistBrain functionPrefrontal cortexHypothalamic AgRP neuronsMedial thalamic neuronsAdministration of clozapineDopamine receptor antagonistVentral tegmental areaOscillatory network activityHigher-order brain functionsHypothalamic agoutiThalamic neuronsChemogenetic inhibitionDopaminergic neuronsReceptor antagonistTegmental areaNeuronal pathwaysSensorimotor gatingAdult miceModulatory impactAmbulatory behaviorConstitutive impairmentNeurons
2021
Integrative Functional Genomic Analysis of Human PTSD Molecular Pathology and Risk
Girgenti M, Skarica M, Zhang J, Wang J, Friedman M, Zhao H, Krystal J. Integrative Functional Genomic Analysis of Human PTSD Molecular Pathology and Risk. Biological Psychiatry 2021, 89: s12. DOI: 10.1016/j.biopsych.2021.02.050.Peer-Reviewed Original Research
2018
Revealing the brain's molecular architecture
Ashley-Koch A, Crawford G, Garrett M, Song L, Safi A, Johnson G, Wray G, Reddy T, Goes F, Zandi P, Bryois J, Jaffe A, Price A, Ivanov N, Collado-Torres L, Hyde T, Burke E, Kleiman J, Tao R, Shin J, Akbarian S, Girdhar K, Jiang Y, Kundakovic M, Brown L, Kassim B, Park R, Wiseman J, Zharovsky E, Jacobov R, Devillers O, Flatow E, Hoffman G, Lipska B, Lewis D, Haroutunian V, Hahn C, Charney A, Dracheva S, Kozlenkov A, Belmont J, DelValle D, Francoeur N, Hadjimichael E, Pinto D, van Bakel H, Roussos P, Fullard J, Bendl J, Hauberg M, Mangravite L, Peters M, Chae Y, Peng J, Niu M, Wang X, Webster M, Beach T, Chen C, Jiang Y, Dai R, Shieh A, Liu C, Grennan K, Xia Y, Vadukapuram R, Wang Y, Fitzgerald D, Cheng L, Brown M, Brown M, Brunetti T, Goodman T, Alsayed M, Gandal M, Geschwind D, Won H, Polioudakis D, Wamsley B, Yin J, Hadzic T, De La Torre Ubieta L, Swarup V, Sanders S, State M, Werling D, An J, Sheppard B, Willsey A, White K, Ray M, Giase G, Kefi A, Mattei E, Purcaro M, Weng Z, Moore J, Pratt H, Huey J, Borrman T, Sullivan P, Giusti-Rodriguez P, Kim Y, Sullivan P, Szatkiewicz J, Rhie S, Armoskus C, Camarena A, Farnham P, Spitsyna V, Witt H, Schreiner S, Evgrafov O, Knowles J, Gerstein M, Liu S, Wang D, Navarro F, Warrell J, Clarke D, Emani P, Gu M, Shi X, Xu M, Yang Y, Kitchen R, Gürsoy G, Zhang J, Carlyle B, Nairn A, Li M, Pochareddy S, Sestan N, Skarica M, Li Z, Sousa A, Santpere G, Choi J, Zhu Y, Gao T, Miller D, Cherskov A, Yang M, Amiri A, Coppola G, Mariani J, Scuderi S, Szekely A, Vaccarino F, Wu F, Weissman S, Roychowdhury T, Abyzov A. Revealing the brain's molecular architecture. Science 2018, 362: 1262-1263. PMID: 30545881, DOI: 10.1126/science.362.6420.1262.Peer-Reviewed Original Research
2017
Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron
Sabolić I, Škarica M, Ljubojević M, Breljak D, Herak-Kramberger C, Crljen V, Ljubešić N. Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron. Journal Of Trace Elements In Medicine And Biology 2017, 46: 62-75. PMID: 29413112, DOI: 10.1016/j.jtemb.2017.11.011.Peer-Reviewed Original ResearchIntersection of diverse neuronal genomes and neuropsychiatric disease: The Brain Somatic Mosaicism Network
McConnell MJ, Moran JV, Abyzov A, Akbarian S, Bae T, Cortes-Ciriano I, Erwin JA, Fasching L, Flasch DA, Freed D, Ganz J, Jaffe AE, Kwan KY, Kwon M, Lodato MA, Mills RE, Paquola ACM, Rodin RE, Rosenbluh C, Sestan N, Sherman MA, Shin JH, Song S, Straub RE, Thorpe J, Weinberger DR, Urban AE, Zhou B, Gage FH, Lehner T, Senthil G, Walsh CA, Chess A, Courchesne E, Gleeson JG, Kidd JM, Park PJ, Pevsner J, Vaccarino FM, Barton A, Bekiranov S, Bohrson C, Burbulis I, Chronister W, Coppola G, Daily K, D’Gama A, Emery S, Frisbie T, Gao T, Gulyás-Kovács A, Haakenson M, Keil J, Kopera H, Lam M, Lee E, Marques-Bonet T, Mathern G, Moldovan J, Oetjens M, Omberg L, Peters M, Pochareddy S, Pramparo T, Ratan A, Sanavia T, Shi L, Skarica M, Wang J, Wang M, Wang Y, Wierman M, Wolpert M, Woodworth M, Zhao X, Zhou W. Intersection of diverse neuronal genomes and neuropsychiatric disease: The Brain Somatic Mosaicism Network. Science 2017, 356 PMID: 28450582, PMCID: PMC5558435, DOI: 10.1126/science.aal1641.Peer-Reviewed Original ResearchConceptsSomatic mutationsComplex genetic architectureStructural genomic variantsNeuronal genomeNeuronal transcriptomeGenetic architectureCell divisionCellular metabolismGenomic variantsLong life spanDNA damageComplex neuropsychiatric disorderCellular expansionNeuropsychiatric diseasesNeuropsychiatric disordersProgenitor cellsSomatic mosaicismIndividual neurodevelopmentSmall populationCell proliferationPopulation-based studyMutationsGermline variantsLife spanBrain development
2013
Immunoregulatory Neuropeptides
Ganea D, Skarica M. Immunoregulatory Neuropeptides. 2013, 144-160. DOI: 10.1002/9781118314814.ch7.Peer-Reviewed Original ResearchVasoactive intestinal peptideCentral nervous systemAnti-inflammatory neuropeptidesImmunoregulatory neuropeptideParasympathetic innervationAutoimmune diseasesImmunomodulatory roleIntestinal peptideImmune cellsLymphoid organsAdaptive immunityVivo effectsNervous systemImmune systemImmune sourcesClinical useNeuropeptidesMajor signaling pathwaysSignaling pathwaysPeptide familyReceptorsPleiotropic effectsInnervationChemokinesCytokines
2011
Modulation of Inflammatory Responses by a Cannabinoid-2–Selective Agonist after Spinal Cord Injury
Adhikary S, Li H, Heller J, Skarica M, Zhang M, Ganea D, Tuma R. Modulation of Inflammatory Responses by a Cannabinoid-2–Selective Agonist after Spinal Cord Injury. Journal Of Neurotrauma 2011, 28: 2417-2427. PMID: 21970496, PMCID: PMC3235339, DOI: 10.1089/neu.2011.1853.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInflammatory responseSpinal cordSelective CB2 agonistAutonomic functionCord injuryMotor functionCB2 agonistsToll-like receptor expressionChemokine/cytokine expressionIL-23p19 expressionCell invasionInflammatory cell invasionSignificant reductionContusion injuryPercentage of animalsCXCL-9Immunoreactive microgliaCXCL-11Cytokine expressionIL-23RReceptor expressionInflammatory reactionInjuryEntire study periodNovel mechanisms of immune modulation of natalizumab in multiple sclerosis patients
Skarica M, Eckstein C, Whartenby K, Calabresi P. Novel mechanisms of immune modulation of natalizumab in multiple sclerosis patients. Journal Of Neuroimmunology 2011, 235: 70-76. PMID: 21550672, DOI: 10.1016/j.jneuroim.2011.02.010.Peer-Reviewed Original ResearchConceptsNatalizumab therapyPeripheral bloodMonth timepointProportion of CD20Proportion of CD4Number of CD14Multiple sclerosis patientsImmune cell compositionHematopoetic stem cellsPercentage of cellsMechanism of actionNatalizumab treatmentRRMS patientsMS patientsNK cellsSclerosis patientsTreatment onsetCytokine productionImmune compositionImmune modulationImmune cellsT cellsB cellsPatientsTherapy
2010
Role of metallothionein in cadmium traffic and toxicity in kidneys and other mammalian organs
Sabolić I, Breljak D, Škarica M, Herak-Kramberger C. Role of metallothionein in cadmium traffic and toxicity in kidneys and other mammalian organs. BioMetals 2010, 23: 897-926. PMID: 20549307, DOI: 10.1007/s10534-010-9351-z.Peer-Reviewed Original ResearchConceptsProximal tubule cellsChronic toxicityTubular injuryRole of metallothioneinMT-1Intracellular MTSquamous epitheliumCytotoxicity of CdChronic intoxicationTubule cellsVariety of stimuliRole of MTKidneyMajor physiological functionsMT-2Oxidative stressAdult mammalsMT-4Acute toxicityMajor targetMT-3Essential metals ZnMost tissuesToxicityDe novo synthesis