CyTOF 2:

Time-of-Flight mass spectrometer for high-speed acquisition of highly multi-parametric single cell data

The CyTOF 2 analyzes cells labeled with stable heavy metal isotopes using state-of-the-art time-of-flight atomic mass cytometry technology. Single-cell experimentation methods provide critical advances for research in cancer, cardiovascular biology, embryonic stem cells and development, hematopoietic stem cells and progenitors, immunity and infectious disease. 

  • Multi-parametric single cell data, deep profiling 
  • Detect up to 42 parameters simultaneously in a single tube (with theoretical limit of 100 parameters) 
  • Detect more from limited samples 
  • Advantages over traditional flow cytometry: No background, no overlap between channels, no compensation issues 
"In house" in our core we maintain:
  • a repository of well-characterized metal conjugated antibodies
  • metal labeling reagents for conjugation of antibodies to these metals: 145Nd, 148Nd, 158Gdm 164Dy, 165Ho, 171Yb.
  • metal conjugated anti-fluorochrome antibodies (e.g. anti-FITC) that can be used with existing fluorescent reagents.
  • CyTOF Antibody Panel Guide
  • Individual metal-conjugated individual antibodies (>500) and panel kits are available from Fluidigm 
  • For purchase of metal-conjugated antibodies (or custom conjugations) from the Longwood CyTOF antibody resource, contact our facility for details.
Available Resources: Please e-mail us for the most updated version
  1. Cell Surface Staining Protocol for CyTOF2 Mass Spectrometry
  2. Sample preparation using barcoding for CyTOF
  3. Useful web links for new users
Biosafety Policies: All infectious, human, and non-human primate cells must be fixed before they are analyzed on the CyTOF. 

Charges: CyTOF analysis is available to Yale University investigators as a fee for service. Non-Yale users will be accommodated as possible. Please contact Dr. Montgomery.