2020
Creatine Transporter, Reduced in Colon Tissues From Patients With Inflammatory Bowel Diseases, Regulates Energy Balance in Intestinal Epithelial Cells, Epithelial Integrity, and Barrier Function
Hall C, Lee J, Murphy E, Gerich M, Dran R, Glover L, Abdulla Z, Skelton M, Colgan S. Creatine Transporter, Reduced in Colon Tissues From Patients With Inflammatory Bowel Diseases, Regulates Energy Balance in Intestinal Epithelial Cells, Epithelial Integrity, and Barrier Function. Gastroenterology 2020, 159: 984-998.e1. PMID: 32433978, PMCID: PMC7891846, DOI: 10.1053/j.gastro.2020.05.033.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsBiopsyCase-Control StudiesCell LineColitis, UlcerativeColonCrohn DiseaseEnergy MetabolismEpithelial CellsFemaleGene Knockdown TechniquesHumansIntestinal MucosaMaleMembrane Transport ProteinsMiceMice, KnockoutMiddle AgedMitochondriaNerve Tissue ProteinsPlasma Membrane Neurotransmitter Transport ProteinsTight JunctionsConceptsInflammatory bowel diseaseIntestinal epithelial cellsColon tissuesTransepithelial electrical resistanceBarrier functionBowel diseaseTight junctionsSeverity of colitisEpithelial cellsInactive Crohn's diseaseIntestinal barrier dysfunctionMucosal biopsy specimensQRT-PCREpithelial integrityQuantitative reverse transcription polymerase chain reactionReverse transcription-polymerase chain reactionIEC cell linesMucosal biopsiesUlcerative colitisCrohn's diseaseControl miceBarrier dysfunctionPolymerase chain reactionBiopsy specimensPatients
2019
Creatine transporter knockout mice (Slc6a8) show increases in serotonin-related proteins and are resilient to learned helplessness
Abdulla ZI, Pennington JL, Gutierrez A, Skelton MR. Creatine transporter knockout mice (Slc6a8) show increases in serotonin-related proteins and are resilient to learned helplessness. Behavioural Brain Research 2019, 377: 112254. PMID: 31542396, DOI: 10.1016/j.bbr.2019.112254.Peer-Reviewed Original ResearchConceptsAffective disordersCreatine transporter knockout miceAffective-like behaviorTail suspension testTransporter knockout miceMonoamine oxidase proteinFemale miceMale miceWildtype miceDeficient miceBehavioral despairEscape latencyCRT expressionKnockout miceMiceSLC6A8DisordersHippocampusFurther investigationCellular bioenergeticsHigh ATP concentrationsCr transporterComplex roleATP concentrationDeletion of the Creatine Transporter (Slc6a8) in Dopaminergic Neurons Leads to Hyperactivity in Mice
Abdulla ZI, Pahlevani B, Lundgren KH, Pennington JL, Udobi KC, Seroogy KB, Skelton MR. Deletion of the Creatine Transporter (Slc6a8) in Dopaminergic Neurons Leads to Hyperactivity in Mice. Journal Of Molecular Neuroscience 2019, 70: 102-111. PMID: 31520365, PMCID: PMC7001766, DOI: 10.1007/s12031-019-01405-w.Peer-Reviewed Original ResearchConceptsCr transporter deficiencyDopaminergic neuronsMotor functionLocomotor activityChallenging beam testSpontaneous activity testMonths of ageAttention deficit hyperactivity disorderCerebral Cr deficiencySpecific neuronal systemsUnique metabolic demandsCerebral creatineHindlimb stepsControl miceNeurological effectsSpontaneous activityMice ageSLC6A8 geneTransporter deficiencyNeurodegenerative disordersDopamine transporterNeuronal systemsMiceCreatine transporterHuntington's diseaseDeletion of the creatine transporter gene in neonatal, but not adult, mice leads to cognitive deficits
Udobi KC, Delcimmuto N, Kokenge AN, Abdulla ZI, Perna MK, Skelton MR. Deletion of the creatine transporter gene in neonatal, but not adult, mice leads to cognitive deficits. Journal Of Inherited Metabolic Disease 2019, 42: 966-974. PMID: 31209903, PMCID: PMC6739135, DOI: 10.1002/jimd.12137.Peer-Reviewed Original ResearchConceptsMorris water mazeKnockout miceSLC6A8 geneWater mazeBrain developmentCrT deficiencyTamoxifen-inducible Cre recombinaseAdministration of tamoxifenNovel object recognitionCreatine transporter geneProper control groupSevere intellectual disabilityBrain CrControl miceAdenosine triphosphateHuman ubiquitin C promoterAdult miceBehavioral testingControl groupPrevalent causeLocomotor activityUbiquitin C promoterCognitive deficitsMiceCre recombinase