2007
Cooperative Unfolding of a Metastable Serpin to a Molten Globule Suggests a Link Between Functional and Folding Energy Landscapes
Tsutsui Y, Wintrode P. Cooperative Unfolding of a Metastable Serpin to a Molten Globule Suggests a Link Between Functional and Folding Energy Landscapes. Journal Of Molecular Biology 2007, 371: 245-255. PMID: 17568610, DOI: 10.1016/j.jmb.2007.05.039.Peer-Reviewed Original ResearchConceptsMutagenesis studiesEquilibrium unfoldingMolten globuleCooperative structural unitDramatic conformational changeMultiple structural domainsNumerous mutagenesis studiesExchange mass spectrometryStable native stateNon-cooperative transitionPrevious mutagenesis studiesMolten globule formHydrogen-deuterium exchangeEquilibrium molten globuleFunctional intermediatesProtease-serpin complexesStructural domainsTarget proteasesConformational changesMetastable SerpinNative stateEquilibrium intermediatesCooperative unfoldingUnfolded stateGlobule form
2006
The Conformational Dynamics of a Metastable Serpin Studied by Hydrogen Exchange and Mass Spectrometry
Tsutsui Y, Liu L, Gershenson A, Wintrode P. The Conformational Dynamics of a Metastable Serpin Studied by Hydrogen Exchange and Mass Spectrometry. Biochemistry 2006, 45: 6561-6569. PMID: 16716066, DOI: 10.1021/bi060431f.Peer-Reviewed Original Research