2008
Lack of a significant role of P-Rex1, a major regulator of macrophage Rac1 activation and chemotaxis, in atherogenesis
Wang Z, Dong X, Li Z, Smith JD, Wu D. Lack of a significant role of P-Rex1, a major regulator of macrophage Rac1 activation and chemotaxis, in atherogenesis. Prostaglandins And Other Lipid Mediators 2008, 87: 9-13. PMID: 18502673, PMCID: PMC3644862, DOI: 10.1016/j.prostaglandins.2008.04.001.Peer-Reviewed Original Research
2007
Phospholipase C β3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
Wang Z, Liu B, Wang P, Dong X, Fernandez-Hernando C, Li Z, Hla T, Li Z, Claffey K, Smith JD, Wu D. Phospholipase C β3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice. Journal Of Clinical Investigation 2007, 118: 195-204. PMID: 18079968, PMCID: PMC2129238, DOI: 10.1172/jci33139.Peer-Reviewed Original ResearchConceptsPLC-beta3PLC-betaPKC-dependent upregulationMacrophage survivalPLC isoformsNovel roleBcl-xLMultiple inducersSubsequent differentiationApoptotic inductionAtherosclerotic plaquesMacrophage apoptosisApoE-deficient mouse modelPLC activityPLC-beta2Murine macrophagesAtherosclerotic lesion sizeReduction of atherosclerosisPotential targetLipid-laden macrophagesTreatment of atherosclerosisBeta3Key membersMacrophage functionApoptosis
2006
Characterization and Application of a New Optical Probe for Membrane Lipid Domains
Jin L, Millard AC, Wuskell JP, Dong X, Wu D, Clark HA, Loew LM. Characterization and Application of a New Optical Probe for Membrane Lipid Domains. Biophysical Journal 2006, 90: 2563-2575. PMID: 16415047, PMCID: PMC1403187, DOI: 10.1529/biophysj.105.072884.Peer-Reviewed Original ResearchConceptsEmission spectraStrong second harmonic generation (SHG) signalNew optical probeSecond harmonic generation signalNonlinear optical microscopyHarmonic generation signalLiquid-disordered phasesLarge unilamellar lipid vesiclesOptical propertiesOptical probeGeneration signalSensitive probeBlue shiftOptical microscopyGiant unilamellar vesiclesLiquid-ordered stateDye moleculesUnilamellar lipid vesiclesLiquid-disordered domainsLipid domainsMolecular packingSpectraLipid membranesRed fluorescencePhase separationMethods for Studying Neutrophil Chemotaxis
Dong X, Wu D. Methods for Studying Neutrophil Chemotaxis. Methods In Enzymology 2006, 406: 605-613. PMID: 16472691, DOI: 10.1016/s0076-6879(06)06047-2.Peer-Reviewed Original Research
2005
P-Rex1 Is a Primary Rac2 Guanine Nucleotide Exchange Factor in Mouse Neutrophils
Dong X, Mo Z, Bokoch G, Guo C, Li Z, Wu D. P-Rex1 Is a Primary Rac2 Guanine Nucleotide Exchange Factor in Mouse Neutrophils. Current Biology 2005, 15: 1874-1879. PMID: 16243036, DOI: 10.1016/j.cub.2005.09.014.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCaseinsCell LineChemotaxisCloning, MolecularEnzyme ActivationGene TargetingGuanine Nucleotide Exchange FactorsHumansImmunoprecipitationMiceMice, KnockoutNeuropeptidesNeutrophilsN-Formylmethionine Leucyl-PhenylalanineProtein TransportRac GTP-Binding ProteinsRac1 GTP-Binding ProteinSuperoxidesConceptsP-Rex1Exchange factorGuanine nucleotide exchange factorsNucleotide exchange factorsRegulation of RacF-actin formationMouse neutrophilsSmall GTPasesRho familyRac activityApparent high affinityRac activatorActin formationRac1 activationRac2 activationCdc42Mouse linesRacRac2RhoAMigration rateRegulationHigh affinityLeukocyte chemoattractantsGTPasesRegulation of PTEN by Rho small GTPases
Li Z, Dong X, Wang Z, Liu W, Deng N, Ding Y, Tang L, Hla T, Zeng R, Li L, Wu D. Regulation of PTEN by Rho small GTPases. Nature Cell Biology 2005, 7: 399-404. PMID: 15793569, DOI: 10.1038/ncb1236.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCdc42 GTP-Binding ProteinChemotaxisDictyosteliumHumansIntracellular Signaling Peptides and ProteinsLeukocytesMiceModels, BiologicalNeutrophilsPhosphoric Monoester HydrolasesProtein Serine-Threonine KinasesProtein TransportPTEN PhosphohydrolaseRhoA GTP-Binding ProteinRho-Associated KinasesTumor Suppressor ProteinsConceptsRegulation of PTENIntracellular localizationEmbryonic kidney cellsSmall GTPaseDownstream effectorsPhospholipid phosphatase activityRho small GTPasesRegulation of chemotaxisKidney cellsHuman embryonic kidney cellsSmall GTPasesRho familyPhosphoinositide substratesKey residuesActive RhoAPhosphatase activityPTENRhoAGTPaseRegulationHuman tumorsCellsDiverse rangeGTPasesCdc42