2023
High dietary K+ intake inhibits proximal tubule transport
Wang T, Liu T, Xu S, Frindt G, Weinstein A, Palmer L. High dietary K+ intake inhibits proximal tubule transport. American Journal Of Physiology. Renal Physiology 2023, 325: f224-f234. PMID: 37318989, PMCID: PMC10396284, DOI: 10.1152/ajprenal.00013.2023.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFree-flow micropunctureFiltration rateUrine volumeFractional NaNephron segmentsOverall kidney functionProximal tubule transportProximal tubule functionNHE3 protein expressionExchanger isoform 3HK animalsKCl dietFraction excretionKidney functionHigh dietaryTubule functionKidney microsomesTubule transportAnesthetized animalsProximal tubulesVolume reabsorptionExcretionWestern blotControl dietHigh Dietary K Intake Inhibits Proximal Tubule Transport and Reduces GFR
Wang T, Liu T, Xu S, Frindt G, Palmer L, Weinstein A. High Dietary K Intake Inhibits Proximal Tubule Transport and Reduces GFR. Physiology 2023, 38: 5731363. DOI: 10.1152/physiol.2023.38.s1.5731363.Peer-Reviewed Original ResearchGlomerular filtration rateTF/PProximal tubulesUrine volumeTubular fluidDistal NaFractional NaReduction of GFRNephron segmentsFree-flow micropunctureOverall kidney functionDistal nephron segmentsProximal nephron segmentsKidney functionFiltration ratePt-NaFiltered loadLate DCTProximal adjustmentsModulation of NaTubule transportFractional deliveryFluid absorptionPT transportRats
2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENaRestoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease
Du Z, Tian X, Ma M, Somlo S, Weinstein AM, Wang T. Restoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease. JCI Insight 2021, 6: e146041. PMID: 33886508, PMCID: PMC8262298, DOI: 10.1172/jci.insight.146041.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateGlomerulotubular balanceRenal cyst formationCyst formationReceptor 1 antagonistPolycystic kidney diseaseKidney weightUntreated miceDA1 antagonistControl miceKidney diseaseFiltration rateFractional reabsorptionCystic indexMouse modelCyst growthConditional KOHCO3- absorptionHeterozygous miceSame antagonistsMicePT transportAntagonistEpithelial ciliaHCO3- transport
2020
Sex Difference In Kidney Electrolyte Transport III: Impact of Low K intake on Thiazide‐Sensitive Cation Excretion in Male and Female Mice
Xu S, Li J, Yang L, Wang C, Liu T, Weinstein A, Palmer L, Wang T. Sex Difference In Kidney Electrolyte Transport III: Impact of Low K intake on Thiazide‐Sensitive Cation Excretion in Male and Female Mice. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.04495.Peer-Reviewed Original ResearchGlomerular filtration rateFemale miceUrine volumeCation excretionNCC proteinNCC activityDietary potassium intakeLow K intakeRole of NCCMeasurement of functionSex differencesNatriuretic responsePotassium intakeK intakeFiltration rateMale miceNCC abundanceNCC expressionDistal tubulesDistal nephronElectrolyte homeostasisLK dietSodium absorptionHCTZK restriction
2012
Effect of Acute Infusion of Low‐Molecular‐Weight Polyvinylpyrrolidone on Renal Function
Yan Q, Du Z, Wan L, Beck F, Giebisch G, Wang T. Effect of Acute Infusion of Low‐Molecular‐Weight Polyvinylpyrrolidone on Renal Function. The FASEB Journal 2012, 26: 1100.2-1100.2. DOI: 10.1096/fasebj.26.1_supplement.1100.2.Peer-Reviewed Original ResearchBlood urea nitrogenGlomerular filtration rateNatriuretic effectRenal functionProximal tubular absorptionPVP storage diseaseLong-term administrationMonocyte/macrophage systemAcute infusionKaliuretic effectRenal damageRenal failureRenal injuryTubular absorptionUrine volumeFiltration rateClearance experimentsUrea nitrogenProximal tubulesInfusionNephron segmentsMacrophage systemElectrolyte absorptionPlasma expanderExcretion
2011
GENDER DIFFERENCE IN MODULATION OF ANGIOTENSIN II TYPE 2 RECEPTOR (AT2) FUNCTION AND EXPRESSION IN AT1A RECEPTOR‐DEFICIENT MICE
Yan Q, Wan L, Du Z, Gotoh N, Wang T. GENDER DIFFERENCE IN MODULATION OF ANGIOTENSIN II TYPE 2 RECEPTOR (AT2) FUNCTION AND EXPRESSION IN AT1A RECEPTOR‐DEFICIENT MICE. The FASEB Journal 2011, 25: 835.9-835.9. DOI: 10.1096/fasebj.25.1_supplement.835.9.Peer-Reviewed Original ResearchGlomerular filtration rateUrine volumeAT2 receptorsProximal tubulesAT1A receptor-deficient miceNull miceExpression levelsReceptor-deficient miceKidneys of malesFemale null miceMale null miceAT2 inhibitorNatriuretic effectRenal functionAT2 expressionWT miceFiltration rateBolus injectionReal-time PCRRenal clearancePD123319ENaC expressionReceptor functionMiceReceptors
2009
Functional expression of the olfactory signaling system in the kidney
Pluznick JL, Zou DJ, Zhang X, Yan Q, Rodriguez-Gil DJ, Eisner C, Wells E, Greer CA, Wang T, Firestein S, Schnermann J, Caplan MJ. Functional expression of the olfactory signaling system in the kidney. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 2059-2064. PMID: 19174512, PMCID: PMC2644163, DOI: 10.1073/pnas.0812859106.Peer-Reviewed Original ResearchConceptsGlomerular filtration ratePlasma renin levelsMacula densa cellsCOX-2 expressionRenal distal nephronOlfactory G-proteinMDS cell linesOlfactory receptorsRenin levelsRenin secretionFiltration rateNNOS activityTubuloglomerular feedbackDistal nephronOlfactory epitheliumRenal tubulesGFR regulationAdenylate cyclaseG proteinsCell linesSensory roleKidneyFunctional expressionOlfactionExpression
2008
Mouse model of type II Bartter's syndrome. I. Upregulation of thiazide-sensitive Na-Cl cotransport activity
Cantone A, Yang X, Yan Q, Giebisch G, Hebert SC, Wang T. Mouse model of type II Bartter's syndrome. I. Upregulation of thiazide-sensitive Na-Cl cotransport activity. American Journal Of Physiology. Renal Physiology 2008, 294: f1366-f1372. PMID: 18385266, DOI: 10.1152/ajprenal.00608.2007.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAmilorideAnimalsBartter SyndromeCation Transport ProteinsChloridesDisease Models, AnimalDiureticsEpithelial Sodium ChannelsFemaleFurosemideGlomerular Filtration RateHydrochlorothiazideLoop of HenleMaleMiceMice, Mutant StrainsPotassium Channels, Inwardly RectifyingPregnancySodiumSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 1Up-RegulationConceptsThick ascending limbExcretion rateWhole kidney glomerular filtration rateKidney glomerular filtration rateFractional excretion rateFurosemide-induced incrementsGlomerular filtration rateType II Bartter's syndromeHyperprostaglandin E syndromeEffect of furosemideDistal nephron segmentsDistal convoluted tubuleExaggerated natriuresisROMK null miceNa excretionFiltration rateRenal saltAbsolute excretionBartter's syndromeE syndromeMouse modelClearance studiesConvoluted tubulesAscending limbNull mice
2007
Blocking angiotensin II type 2 receptor (AT2) induces diuresis and natriuresis in AT1A receptor‐deficient mice
Yan Q, Du Z, Duan Y, Weinbaum S, Weinstein A, Wang T. Blocking angiotensin II type 2 receptor (AT2) induces diuresis and natriuresis in AT1A receptor‐deficient mice. The FASEB Journal 2007, 21: a1246-a1246. DOI: 10.1096/fasebj.21.6.a1246-c.Peer-Reviewed Original ResearchMean arterial blood pressureGlomerular filtration rateAT1A receptor-deficient miceReceptor-deficient miceUrine volumeAT2 receptorsLower mean arterial blood pressureAngiotensin II type 2 receptorAdministration of PD123319Renin-angiotensin systemArterial blood pressureType 2 receptorBlocker PD123319Blood pressureIntravenous bolusFiltration rateKO miceRenal handlingRenal clearancePD123319Receptor functionFluid homeostasisWater handlingReceptorsMice
1998
A tyrosine-based signal regulates H-K-ATPase-mediated potassium reabsorption in the kidney
Wang T, Courtois-Coutry N, Giebisch G, Caplan M. A tyrosine-based signal regulates H-K-ATPase-mediated potassium reabsorption in the kidney. American Journal Of Physiology 1998, 275: f818-f826. PMID: 9815140, DOI: 10.1152/ajprenal.1998.275.5.f818.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateTransgenic miceGastric acid outputPlasma K concentrationK pumpK-ATPaseRenal collecting tubulesK clearanceBlood pressurePotassium reabsorptionAcid outputUrine volumeK excretionFiltration rateGastric acidK reabsorptionPump functionCollecting tubuleMicePlasma NaTyrosine-based sequenceTyrosine-based signalsKidneyExcretionCytoplasmic tail
1997
Nitric oxide regulates HCO3- and Na+ transport by a cGMP-mediated mechanism in the kidney proximal tubule
Wang T. Nitric oxide regulates HCO3- and Na+ transport by a cGMP-mediated mechanism in the kidney proximal tubule. American Journal Of Physiology 1997, 272: f242-f248. PMID: 9124402, DOI: 10.1152/ajprenal.1997.272.2.f242.Peer-Reviewed Original ResearchConceptsSodium nitroprussideProximal tubulesKidney proximal tubulesBlood pressureL-NAMENitric oxideNitro-L-arginine methyl esterNitric oxide synthase inhibitorGlomerular filtration rateOxide synthase inhibitorGuanylate cyclase inhibitorUrine flow rateMicroM sodium nitroprussideRenal hemodynamicsFiltration rateRenal excretionRenal clearanceSynthase inhibitorCyclase inhibitorLuminal perfusateRat kidneyMicroperfusion techniqueStimulatory effectSimilar stimulationCyclic monophosphate