2021
Non-Genetic Diversity in Chemosensing and Chemotactic Behavior
Moore JP, Kamino K, Emonet T. Non-Genetic Diversity in Chemosensing and Chemotactic Behavior. International Journal Of Molecular Sciences 2021, 22: 6960. PMID: 34203411, PMCID: PMC8268644, DOI: 10.3390/ijms22136960.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacteriaBacterial ProteinsChemotactic FactorsChemotaxisHumansSignal Transduction
2020
Adaptive tuning of cell sensory diversity without changes in gene expression
Kamino K, Keegstra JM, Long J, Emonet T, Shimizu TS. Adaptive tuning of cell sensory diversity without changes in gene expression. Science Advances 2020, 6: eabc1087. PMID: 33188019, PMCID: PMC7673753, DOI: 10.1126/sciadv.abc1087.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalBacterial ProteinsChemotaxisEscherichia coliEscherichia coli ProteinsGene ExpressionConceptsPhenotypic diversityGene expressionCell variationDiversity of phenotypesChemoreceptor TarPosttranslational modificationsAllosteric couplingEnvironmental cuesChemotaxis networkCovalent modificationEnvironmental changesDiversitySensory diversityCell populationsCellsExpressionSuch betsPhenotypeEscherichiaPopulationPrevious studiesModificationVariationSignalsChemoreceptors
2014
Limits of Feedback Control in Bacterial Chemotaxis
Dufour YS, Fu X, Hernandez-Nunez L, Emonet T. Limits of Feedback Control in Bacterial Chemotaxis. PLOS Computational Biology 2014, 10: e1003694. PMID: 24967937, PMCID: PMC4072517, DOI: 10.1371/journal.pcbi.1003694.Peer-Reviewed Original ResearchConceptsFeedback controlIntegral feedback controlOperational regimesOptimal operational regimeComplex statisticsDrift velocityRobust performanceFuture inputsAdaptation rateProper information transferChemotactic driftAnalytical modelInput signalOptimal regimeActuatorsSteep gradientsProper couplingWide rangeChemotactic performanceRegimeBifurcationAdaptability of non-genetic diversity in bacterial chemotaxis
Frankel NW, Pontius W, Dufour YS, Long J, Hernandez-Nunez L, Emonet T. Adaptability of non-genetic diversity in bacterial chemotaxis. ELife 2014, 3: e03526. PMID: 25279698, PMCID: PMC4210811, DOI: 10.7554/elife.03526.Peer-Reviewed Original ResearchConceptsGene regulationNon-genetic diversityBacterial chemotaxis systemHeritable controlChemotaxis systemSelectable traitAdvantageous diversityEnvironmental variationBacterial chemotaxisEnvironmental variabilityClonal populationsDiverse environmentsDiversityE. coliProtein levelsMutationsDifferent environmentsRegulationForagingTraitsColiDiversificationBacteriaColonizationPopulation
2010
Fine-Tuning of Chemotactic Response in E. coli Determined by High-Throughput Capillary Assay
Park H, Guet CC, Emonet T, Cluzel P. Fine-Tuning of Chemotactic Response in E. coli Determined by High-Throughput Capillary Assay. Current Microbiology 2010, 62: 764-769. PMID: 20972792, PMCID: PMC3230253, DOI: 10.1007/s00284-010-9778-z.Peer-Reviewed Original ResearchConceptsChemotactic responseWild-type levelsCapillary assayRobust chemotactic responseE. coliWild-type behaviorE. coli cellsPerfect adaptationDepletion of nutrientsColi cellsFusion proteinCellular growthChemotactic behaviorProteinIntracellular concentrationChemotactic proteinColiAssaysChemotaxisAdaptationSwimming bacteriaCheB.Effects of growthCheRGrowthSpatial organization of the flow of genetic information in bacteria
Montero Llopis P, Jackson AF, Sliusarenko O, Surovtsev I, Heinritz J, Emonet T, Jacobs-Wagner C. Spatial organization of the flow of genetic information in bacteria. Nature 2010, 466: 77-81. PMID: 20562858, PMCID: PMC2896451, DOI: 10.1038/nature09152.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCaulobacter crescentusChaperoninsChromosomes, BacterialDiffusionDNA, BacterialEndoribonucleasesEscherichia coliGene Expression Regulation, BacterialIn Situ Hybridization, FluorescenceLac OperonProtein BiosynthesisRibosomesRNA StabilityRNA TransportRNA, BacterialRNA, MessengerTranscription, GeneticConceptsSites of transcriptionC. crescentusCaulobacter crescentusEukaryotic cellsCellular physiologyMRNA decayMature mRNAMRNA processesRNase EMRNA substratesMRNA localizationGenetic informationGene expressionBacterial cellsEscherichia coliQuantitative fluorescenceCrescentusSitu hybridizationSpatial organizationMRNABacteriaLimited dispersionCellsTranscriptionTranslationProcessivity of peptidoglycan synthesis provides a built-in mechanism for the robustness of straight-rod cell morphology
Sliusarenko O, Cabeen MT, Wolgemuth CW, Jacobs-Wagner C, Emonet T. Processivity of peptidoglycan synthesis provides a built-in mechanism for the robustness of straight-rod cell morphology. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 10086-10091. PMID: 20479277, PMCID: PMC2890421, DOI: 10.1073/pnas.1000737107.Peer-Reviewed Original Research
2009
RodZ, a component of the bacterial core morphogenic apparatus
Alyahya SA, Alexander R, Costa T, Henriques AO, Emonet T, Jacobs-Wagner C. RodZ, a component of the bacterial core morphogenic apparatus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 1239-1244. PMID: 19164570, PMCID: PMC2633561, DOI: 10.1073/pnas.0810794106.Peer-Reviewed Original ResearchConceptsCell morphogenesisExtracellular C-terminal domainBacterial cell morphogenesisConservation of functionTerminal cytoplasmic domainC-terminal domainN-terminal domainMultiple sequence alignmentMreB cytoskeletonBacterial kingdomAncient functionCaulobacter crescentusGrowth machineryLocalization determinantsHelix motifRodZTransmembrane sequenceBacterial phylaCytoplasmic domainGenomic analysisTerminal domainMolecular basisPeptidoglycan synthesisCellular formCell cycle
2006
Hidden Stochastic Nature of a Single Bacterial Motor
Korobkova EA, Emonet T, Park H, Cluzel P. Hidden Stochastic Nature of a Single Bacterial Motor. Physical Review Letters 2006, 96: 058105. PMID: 16486999, DOI: 10.1103/physrevlett.96.058105.Peer-Reviewed Original Research
2005
Real-time RNA profiling within a single bacterium
Le TT, Harlepp S, Guet C, Dittmar K, Emonet T, Pan T, Cluzel P. Real-time RNA profiling within a single bacterium. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 9160-9164. PMID: 15967986, PMCID: PMC1166617, DOI: 10.1073/pnas.0503311102.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell CycleCell ProliferationDose-Response Relationship, DrugDrug Resistance, MultipleEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialGenes, ReporterGenotypeGreen Fluorescent ProteinsModels, StatisticalPlasmidsPromoter Regions, GeneticProtein BindingRecombinant Fusion ProteinsRNARNA, MessengerSpectrometry, FluorescenceSpliceosomesTime FactorsTranscription, GeneticConceptsRNA profilingTranscriptional dynamicsInducible gene expression systemGene expression systemSingle cellsSpecific RNA levelsSingle bacteriumRNA levelsGenetic systemSpecific promotersFluorescence correlation spectroscopyExpression systemEscherichia coliEfflux pump systemGenetic deletionProfilingBacteriumPopulation measurementsKey determinantAntibiotic resistanceProkaryotesCellsPromoterCorrelation spectroscopyRNA