2022
Culture-Independent Detection and Identification of Leptospira Serovars
Matthias M, Lubar A, Acharige S, Chaiboonma K, Pilau N, Marroquin A, Jayasundara D, Agampodi S, Vinetz J. Culture-Independent Detection and Identification of Leptospira Serovars. Microbiology Spectrum 2022, 10: e02475-22. PMID: 36445143, PMCID: PMC9769591, DOI: 10.1128/spectrum.02475-22.Peer-Reviewed Original ResearchConceptsPublic health importanceProspective studyFatal diseaseHealth importanceOutbreak investigationSequence typingDog urine samplesCulture-independent assaysEnvironmental surveillance studyMultilocus sequence typingPulsed-field gel electrophoresisSurveillance studyGroup IIHuman leptospirosisPrognostic testSpecific real-time qPCRClinical settingGroup ILeptospira serovarsReal-time qPCRDiagnostic testsSerum samplesUrine samplesDiagnostic specificityLeptospirosisDiagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting
Warnasekara J, Srimantha S, Kappagoda C, Jayasundara D, Senevirathna I, Matthias M, Agampodi S, Vinetz J. Diagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting. PLOS Neglected Tropical Diseases 2022, 16: e0010331. PMID: 35377883, PMCID: PMC9009773, DOI: 10.1371/journal.pntd.0010331.Peer-Reviewed Original ResearchConceptsMicroscopic agglutination testProspective hospital-based studyLeptospirosis casesQuantitative PCRHigh endemic settingHospital-based studyClinical case definitionLarge prospective studiesDisease burden estimatesPublic health burdenStandard diagnostic testMonths of observationUndifferentiated feverAcute leptospirosisProspective studyCase definitionEndemic settingsHealth burdenTimely diagnosisBurden estimatesDiagnostic valueSignificant human mortalityAgglutination testStudy settingDiagnostic testsComparison of global definitions of metabolic syndrome in early pregnancy among the Rajarata Pregnancy Cohort participants in Sri Lanka
Jayasinghe IU, Agampodi TC, Dissanayake AK, Srimantha SM, Agampodi SB. Comparison of global definitions of metabolic syndrome in early pregnancy among the Rajarata Pregnancy Cohort participants in Sri Lanka. Scientific Reports 2022, 12: 2009. PMID: 35132136, PMCID: PMC8821546, DOI: 10.1038/s41598-022-05919-z.Peer-Reviewed Original ResearchConceptsPeriod of gestationTotal cholesterolEarly pregnancyMetabolic syndromeMetabolic derangementsCohort participantsPrevalence estimatesOral glucose tolerance testHigher MetS prevalenceNCEP ATP IIIAHA/NHLBIGlucose tolerance testDifferent diagnostic criteriaSignificant differencesCross-sectional analysisAge 27.9MetS prevalencePregnancy outcomesSerum lipidsMetS definitionsProspective studyPregnant womenPlasma glucoseTolerance testMetabolic parameters
2021
12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka
Jayasundara D, Senavirathna I, Warnasekara J, Gamage C, Siribaddana S, Kularatne SAM, Matthias M, Mariet JF, Picardeau M, Agampodi S, Vinetz J. 12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka. PLOS Neglected Tropical Diseases 2021, 15: e0009272. PMID: 33735202, PMCID: PMC8009393, DOI: 10.1371/journal.pntd.0009272.Peer-Reviewed Original ResearchConceptsClonal groupsAcute kidney injuryL. interrogansMajor clinical challengeGlobal disease threatsLeptospira speciesL. interrogans infectionKidney injuryLiver impairmentCardiovascular involvementL. borgpeterseniiClinical presentationProspective studyClinical severityClinical challengePathogenic Leptospira speciesAnnual epidemicsUbiquitous zoonotic diseaseNew serogroupEpidemiological contextZoonotic diseaseCulture isolationLeptospiral culturesL. weiliiLeptospira diversity
2020
Cytokine response in human leptospirosis with different clinical outcomes: a systematic review
Senavirathna I, Rathish D, Agampodi S. Cytokine response in human leptospirosis with different clinical outcomes: a systematic review. BMC Infectious Diseases 2020, 20: 268. PMID: 32264832, PMCID: PMC7137275, DOI: 10.1186/s12879-020-04986-9.Peer-Reviewed Original ResearchConceptsDifferent clinical outcomesCross-sectional studyCytokine responsesIL-10Clinical outcomesSystematic reviewDisease severityHealth Quality Assessment ToolComparative cross-sectional studyTNF-α levelsMethodsA systematic reviewMeasurement of cytokinesEnzyme-linked immunosorbent assayMicroscopic agglutination testPublic health professionalsQuality assessment toolMild leptospirosisCytokine stormElevated cytokinesObservational cohortLeptospirosis patientsProspective studyIL-1βIL-6IL-8
2019
Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study
Agampodi S, Warnasekara J, Jayasundara D, Senawirathna I, Gamage C, Kularatne S, Siribaddana S, Maththias M, Vinetz J. Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study. BMJ Open 2019, 9: e027850. PMID: 31511281, PMCID: PMC6738675, DOI: 10.1136/bmjopen-2018-027850.Peer-Reviewed Original ResearchConceptsEthics Review CommitteeMultilocus sequence typingOutpatient departmentClinico-epidemiological studyMulticentre prospective studyDay of admissionMicroscopic agglutination testPresence of diseaseHospital stayFebrile patientsMedical wardsProspective studySri Lankan isolatesRisk factorsVenous bloodDisease progressionEthical clearanceHigh incidenceAetiological aspectsPatientsAgglutination testMolecular epidemiologyPatient contextBedside inoculationLeptospirosis
2015
The 2013 measles outbreak in Sri Lanka: experience from a rural district and implications for measles elimination goals
Dahanayaka NJ, Pahalagamage S, Ganegama RM, Weerawansa P, Agampodi SB. The 2013 measles outbreak in Sri Lanka: experience from a rural district and implications for measles elimination goals. Infectious Diseases Of Poverty 2015, 4: 51. PMID: 26627462, PMCID: PMC4667465, DOI: 10.1186/s40249-015-0084-7.Peer-Reviewed Original ResearchConceptsMeasles elimination goalElimination goalsMeasles immunization schedulePosterior cervical lymphadenopathyCommon clinical featuresIntensive care unitUniversity Medical UnitMonths of ageBasic demographic detailsCervical lymphadenopathyKoplik's spotsMaculopapular rashImmunization statusPrior immunizationVaccine scheduleCare unitClinical featuresImmunization scheduleMeasles vaccineSevere pneumoniaMeasles immunizationProspective studyRespiratory difficultyRubella vaccineEpidemiological profileMassive outbreak of measles in 2013, an experience in Teaching Hospital Anuradhapura
Ganegama R, Arachchi N, Senevirathne S, Karunamuni N, Agampodi S, Dahanayaka N, Prasanna P, Pahalagamage S. Massive outbreak of measles in 2013, an experience in Teaching Hospital Anuradhapura. Anuradhapura Medical Journal 2015, 9: 41. DOI: 10.4038/amj.v9i2supp.7590.Peer-Reviewed Original ResearchTeaching Hospital AnuradhapuraMeasles outbreakMeasles incidenceClinical-epidemiological profileTypical clinical featuresProgram of immunizationUniversity Medical UnitAge group 12Months of ageICU careKoplik's spotsDose vaccineImmunization statusPrior immunizationClinical featuresImmunization scheduleMeasles vaccineSevere pneumoniaMeasles immunizationProspective studyRespiratory difficultyMeasles patientsSingle doseClinical studiesEpidemiology Unit
2014
Hantavirus infection mimicking leptospirosis: how long are we going to rely on clinical suspicion?
Dahanayaka NJ, Agampodi SB, Bandaranayaka AK, Priyankara S, Vinetz JM. Hantavirus infection mimicking leptospirosis: how long are we going to rely on clinical suspicion? The Journal Of Infection In Developing Countries 2014, 8: 1072-5. PMID: 25116678, DOI: 10.3855/jidc.4115.Peer-Reviewed Original ResearchConceptsHantavirus infectionPopulation-based prospective studyDiagnostic facilitiesAcute hantavirus infectionClassical clinical pictureCases of leptospirosisClinical suspicionClinical pictureProspective studyEpidemiological featuresRetrospective analysisInfectious diseasesInfectionSerum samplesMedical staffLeptospirosisLeptospirosis outbreaksFeverDiseaseDiagnosisSuspicionSri Lanka