2015
Parasitological Confirmation and Analysis of Leishmania Diversity in Asymptomatic and Subclinical Infection following Resolution of Cutaneous Leishmaniasis
Rosales-Chilama M, Gongora RE, Valderrama L, Jojoa J, Alexander N, Rubiano LC, Cossio A, Adams ER, Saravia NG, Gomez MA. Parasitological Confirmation and Analysis of Leishmania Diversity in Asymptomatic and Subclinical Infection following Resolution of Cutaneous Leishmaniasis. PLOS Neglected Tropical Diseases 2015, 9: e0004273. PMID: 26659114, PMCID: PMC4684356, DOI: 10.1371/journal.pntd.0004273.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAsymptomatic InfectionsBlotting, SouthernChildCluster AnalysisColombiaDNA, HelminthDNA, KinetoplastFemaleGenetic VariationGenotypeHumansLeishmaniaLeishmaniasis, CutaneousMaleMiddle AgedMolecular Sequence DataPhylogenyPolymerase Chain ReactionRNA, Small CytoplasmicSequence Analysis, DNASignal Recognition ParticleYoung AdultConceptsCutaneous leishmaniasisSubclinical infectionParasitological confirmationAsymptomatic infectionEndemic areasHistory of CLTest-positive individualsImmunological evidenceViability of LeishmaniaMucosal swab samplesPersistent subclinical infectionMucosal tissue samplesReservoir of infectionActive diseaseLeishmania kDNALeishmania infectionPositive individualsPersistent infectionBlood monocytesParasite burdenInfectionParasite populationsSwab samplesTransmission of diseaseTissue samples
2009
Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species
Figueroa RA, Lozano LE, Romero IC, Cardona MT, Prager M, Pacheco R, Diaz YR, Tellez JA, Saravia NG. Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species. The Journal Of Infectious Diseases 2009, 200: 638-646. PMID: 19569974, PMCID: PMC5056532, DOI: 10.1086/600109.Peer-Reviewed Original ResearchConceptsKinetoplast minicircle DNAMucosal tissuesCutaneous leishmaniasisMucocutaneous leishmaniasisNasal mucosaSwab samplesMucosa of patientsLeishmania speciesPresence of LeishmaniaDetection of LeishmaniaMucosal leishmaniasisMucosal diseaseCutaneous diseaseLeishmania infectionPolymerase chain reactionParasitological evaluationPatientsLeishmania panamensisLeishmania guyanensisMucosaNatural historyLeishmaniasisLeishmania braziliensisChain reactionAsymptomatic presence
2005
Amplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction.
Vergel C, Walker J, Saravia NG. Amplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction. American Journal Of Tropical Medicine And Hygiene 2005, 72: 423-9. PMID: 15827280, DOI: 10.4269/ajtmh.2005.72.423.Peer-Reviewed Original ResearchConceptsMolecular amplification techniquesPerformance of primersPolymerase chain reaction productsAmplification techniquesMonocyte DNADNA probesLeishmania kinetoplast DNANew applicationsChain reaction productsDNA amplificationHuman DNA sequencesDNA sequencesDetection of parasitesPolymerase chain reaction
2000
INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA
Pacheco R, Fernandes O, Salinas G, Segura I, Momen H, Degrave W, Saravia N, Campbell D. INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA. Journal Of Parasitology 2000, 86: 1250-1253. PMID: 11191900, DOI: 10.1645/0022-3395(2000)086[1250:ihitme]2.0.co;2.Peer-Reviewed Original ResearchConceptsMini-exon gene sequencesGene sequencesGene localizationMini-exon geneChromosomal localizationGenetic variabilityL. guyanensisIntraspecific heterogeneityDifferent karyotypesChromosomesPanamensisKbL. panamensisSpeciesLeishmania panamensisMolecular epidemiologic markerViannia subgenusHuman isolates