2004
Roles of the Proline-rich Domain in SLP-76 Subcellular Localization and T Cell Function* [boxs]
Singer AL, Bunnell SC, Obstfeld AE, Jordan MS, Wu JN, Myung PS, Samelson LE, Koretzky GA. Roles of the Proline-rich Domain in SLP-76 Subcellular Localization and T Cell Function* [boxs]. Journal Of Biological Chemistry 2004, 279: 15481-15490. PMID: 14722089, DOI: 10.1074/jbc.m313339200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAlanineAntigens, CDAntigens, Differentiation, T-LymphocyteArginineBlotting, WesternCalciumCell LineCell LineageFlow CytometryGene DeletionGenes, DominantHematopoietic Stem CellsHumansJurkat CellsLectins, C-TypeLuciferasesLymphocyte ActivationLysineMembrane MicrodomainsModels, BiologicalMutationPhosphoproteinsPlasmidsPrecipitin TestsProlineProtein Structure, TertiarySignal TransductionSrc Homology DomainsSubcellular FractionsTime FactorsT-LymphocytesTransfectionConceptsSLP-76P1 domainSLP-76 functionProline-rich domainProline-rich regionDomain deletion mutantMultiple hematopoietic lineagesLeukocyte-specific phosphoproteinSrc homologyDeletion mutantsSignal transductionSubcellular localizationHematopoietic lineagesFunctional roleProtein fragmentsT cell receptorMolecular scaffoldsCell functionCell receptorLocalizationComplementary approachesDomainT cell functionMolecular associationDirect evidence
2003
Inhibition of T cell activation through interruption of adaptor protein associations
Singer A, Bunnell S, Obstfeld A, Jordan M, Wu J, Myung P, Samelson L, Koretzky G. Inhibition of T cell activation through interruption of adaptor protein associations. Journal Of Surgical Research 2003, 114: 271. DOI: 10.1016/j.jss.2003.08.227.Peer-Reviewed Original ResearchSLP-76NF-ATSLP-76 functionTCR engagementT cell receptorMultiple hematopoietic lineagesLive confocal microscopyJurkat T cellsAdaptor LATInducible associationSecond messenger pathwaysBinding partnerComplex assemblyProtein associationBiochemical signalsHematopoietic lineagesAdaptor moleculeFusion proteinImmune synapseTCR stimulationJurkat cellsMessenger pathwaysCell membraneT cell activationBinding fragment