2019
Regulation of axon growth by myosin II–dependent mechanocatalysis of cofilin activity
Zhang XF, Ajeti V, Tsai N, Fereydooni A, Burns W, Murrell M, De La Cruz EM, Forscher P. Regulation of axon growth by myosin II–dependent mechanocatalysis of cofilin activity. Journal Of Cell Biology 2019, 218: 2329-2349. PMID: 31123185, PMCID: PMC6605792, DOI: 10.1083/jcb.201810054.Peer-Reviewed Original Research
2016
Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation
Buck KB, Schaefer AW, Schoonderwoert VT, Creamer MS, Dufresne ER, Forscher P. Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation. Molecular Biology Of The Cell 2016, 28: 98-110. PMID: 27852899, PMCID: PMC5221634, DOI: 10.1091/mbc.e16-04-0228.Peer-Reviewed Original Research
2014
Dynamic peripheral traction forces balance stable neurite tension in regenerating Aplysia bag cell neurons
Hyland C, Mertz AF, Forscher P, Dufresne E. Dynamic peripheral traction forces balance stable neurite tension in regenerating Aplysia bag cell neurons. Scientific Reports 2014, 4: 4961. PMID: 24825441, PMCID: PMC4019958, DOI: 10.1038/srep04961.Peer-Reviewed Original ResearchRegeneration of Aplysia Bag Cell Neurons is Synergistically Enhanced by Substrate-Bound Hemolymph Proteins and Laminin
Hyland C, Dufresne ER, Forscher P. Regeneration of Aplysia Bag Cell Neurons is Synergistically Enhanced by Substrate-Bound Hemolymph Proteins and Laminin. Scientific Reports 2014, 4: 4617. PMID: 24722588, PMCID: PMC3983596, DOI: 10.1038/srep04617.Peer-Reviewed Original ResearchConceptsBag cell neuronsHemolymph proteinsRespiratory protein hemocyaninAplysia bag cell neuronsProtein complexesFurther molecular characterizationAddition of hemolymphHigh molecular weight proteinsCell neuronsMolecular weight proteinsMolecular characterizationCellular targetsExtracellular matrixProteinNervous system repairNovel synergistic effectWeight proteinsLaminin substrateHumoral proteinsLamininPossible cooperationActive factorsMigration rateEndogenous factorsPotential relevance
2013
Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks
Mejean CO, Schaefer AW, Buck KB, Kress H, Shundrovsky A, Merrill JW, Dufresne ER, Forscher P. Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks. PLOS ONE 2013, 8: e73389. PMID: 24039928, PMCID: PMC3765355, DOI: 10.1371/journal.pone.0073389.Peer-Reviewed Original ResearchProtein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
Yang Q, Zhang XF, Van Goor D, Dunn AP, Hyland C, Medeiros N, Forscher P. Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones. Molecular Biology Of The Cell 2013, 24: 3097-3114. PMID: 23966465, PMCID: PMC3784383, DOI: 10.1091/mbc.e13-05-0289.Peer-Reviewed Original ResearchConceptsProtein kinase CMyosin II contractilityActin network densityNeuronal growth conesPKC activationCentral cytoplasmic domainRetrograde actin network flowTwo-tiered mechanismEffect of PKCActin network flowActin network structureActin filament networkGrowth conesProtein kinase C activationKinase C activationCytoplasmic domainActin polymerizationKinase CFilament networkCytoskeletal mechanismsRegulatory light chain phosphorylationPKC actionPKC activityC activationGuidance responses
2012
Calcineurin-dependent cofilin activation and increased retrograde actin flow drive 5-HT–dependent neurite outgrowth in Aplysia bag cell neurons
Zhang XF, Hyland C, Van Goor D, Forscher P. Calcineurin-dependent cofilin activation and increased retrograde actin flow drive 5-HT–dependent neurite outgrowth in Aplysia bag cell neurons. Molecular Biology Of The Cell 2012, 23: 4833-4848. PMID: 23097492, PMCID: PMC3521690, DOI: 10.1091/mbc.e12-10-0715.Peer-Reviewed Original ResearchConceptsPhospholipase CNeurite outgrowthDynamic cytoskeletal processesRetrograde actin network flowP domainRetrograde actin flowActin network flowSoluble growth factorsAplysia bag cell neuronsBag cell neuronsCofilin activityWidespread mechanismCytoskeletal processesActin flowCofilin activationCell neuronsNeurite outgrowth rateMechanistic roleInositol trisphosphateOutgrowthGrowth factorDirect activationOutgrowth rateBasal levelsActivationArp2/3 complex–dependent actin networks constrain myosin II function in driving retrograde actin flow
Yang Q, Zhang XF, Pollard TD, Forscher P. Arp2/3 complex–dependent actin networks constrain myosin II function in driving retrograde actin flow. Journal Of Cell Biology 2012, 197: 939-956. PMID: 22711700, PMCID: PMC3384413, DOI: 10.1083/jcb.201111052.Peer-Reviewed Original Research
2008
Myosin II Activity Facilitates Microtubule Bundling in the Neuronal Growth Cone Neck
Burnette DT, Ji L, Schaefer AW, Medeiros NA, Danuser G, Forscher P. Myosin II Activity Facilitates Microtubule Bundling in the Neuronal Growth Cone Neck. Developmental Cell 2008, 15: 163-169. PMID: 18606149, PMCID: PMC2548298, DOI: 10.1016/j.devcel.2008.05.016.Peer-Reviewed Original ResearchCoordination of Actin Filament and Microtubule Dynamics during Neurite Outgrowth
Schaefer AW, Schoonderwoert VT, Ji L, Mederios N, Danuser G, Forscher P. Coordination of Actin Filament and Microtubule Dynamics during Neurite Outgrowth. Developmental Cell 2008, 15: 146-162. PMID: 18606148, PMCID: PMC2595147, DOI: 10.1016/j.devcel.2008.05.003.Peer-Reviewed Original ResearchConceptsRetrograde actin network flowActin network flowNeurite outgrowthMyosin II contractilityRho-kinaseCell adhesion substratesCytoskeletal dynamicsMicrotubule behaviorAdhesion sitesAdhesion substrateMicrotubule dynamicsMyosin IIActin filamentsMolecular eventsGrowth responseMicrotubule movementKinaseNeuronal growthMicrotubulesOutgrowthGrowth cone neckGrowthOrganellesRegulationCascade
2007
Filopodial actin bundles are not necessary for microtubule advance into the peripheral domain of Aplysia neuronal growth cones
Burnette DT, Schaefer AW, Ji L, Danuser G, Forscher P. Filopodial actin bundles are not necessary for microtubule advance into the peripheral domain of Aplysia neuronal growth cones. Nature Cell Biology 2007, 9: 1360-1369. PMID: 18026092, DOI: 10.1038/ncb1655.Peer-Reviewed Original Research
2006
Myosin II functions in actin-bundle turnover in neuronal growth cones
Medeiros NA, Burnette DT, Forscher P. Myosin II functions in actin-bundle turnover in neuronal growth cones. Nature Cell Biology 2006, 8: 216-226. PMID: 16501565, DOI: 10.1038/ncb1367.Peer-Reviewed Original Research
2001
Transmission of growth cone traction force through apCAM–cytoskeletal linkages is regulated by Src family tyrosine kinase activity
Suter D, Forscher P. Transmission of growth cone traction force through apCAM–cytoskeletal linkages is regulated by Src family tyrosine kinase activity. Journal Of Cell Biology 2001, 155: 427-438. PMID: 11673478, PMCID: PMC2150837, DOI: 10.1083/jcb.200107063.Peer-Reviewed Original ResearchProtein Kinase C Activation Promotes Microtubule Advance in Neuronal Growth Cones by Increasing Average Microtubule Growth Lifetimes
Kabir N, Schaefer A, Nakhost A, Sossin W, Forscher P. Protein Kinase C Activation Promotes Microtubule Advance in Neuronal Growth Cones by Increasing Average Microtubule Growth Lifetimes. Journal Of Cell Biology 2001, 152: 1033-1044. PMID: 11238458, PMCID: PMC2198821, DOI: 10.1083/jcb.152.5.1033.Peer-Reviewed Original Research
1998
Binding of Protein Kinase C Isoforms to Actin in Aplysia
Nakhost A, Forscher P, Sossin W. Binding of Protein Kinase C Isoforms to Actin in Aplysia. Journal Of Neurochemistry 1998, 71: 1221-1231. PMID: 9721748, DOI: 10.1046/j.1471-4159.1998.71031221.x.Peer-Reviewed Original ResearchConceptsPKC Apl IIProtein kinase C isoformsActin filamentsTriton-insoluble fractionApl IIF-actinCytoskeletal dynamicsC isoformsPhorbol ester-dependent mannerPKC Apl IMarine mollusk Aplysia californicaPhorbol ester treatmentNeuronal growth conesMollusk Aplysia californicaPKC phosphorylationApl IPKC-epsilonPhysiological roleIndependent PKCStriking colocalizationPKC inhibitorKinasePKC betaIIEster treatmentPKCThe Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate–Cytoskeletal Coupling
Suter D, Errante L, Belotserkovsky V, Forscher P. The Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate–Cytoskeletal Coupling. Journal Of Cell Biology 1998, 141: 227-240. PMID: 9531561, PMCID: PMC2132711, DOI: 10.1083/jcb.141.1.227.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAplysiaCell Adhesion MoleculesCell MovementCells, CulturedCloning, MolecularCytoskeletonDNA PrimersGanglia, InvertebrateImmunoglobulin GKineticsMicroscopy, VideoMicrotubulesModels, BiologicalNeuronsPolymerase Chain ReactionRecombinant ProteinsSilicon DioxideStress, MechanicalTubulin
1992
Novel form of growth cone motility involving site-directed actin filament assembly
Forscher P, Lin C, Thompson C. Novel form of growth cone motility involving site-directed actin filament assembly. Nature 1992, 357: 515-518. PMID: 1608453, DOI: 10.1038/357515a0.Peer-Reviewed Original ResearchConceptsActin filament assemblyFilament assemblyNeuronal growth conesCortical F-actin networkMembrane-cytoskeletal interfaceF-actin assemblyF-actin networkGrowth conesGrowth cone migrationGrowth cone motilityExtracellular signalsBacterial parasiteMembrane proteinsCell movementCytoskeletal structuresIntracellular movementDevelopmental processesBead movementCell locomotionCone migrationMorphogenic changesMotile cellsAxonal guidanceCone motilityCellular mechanisms