2019
Regulation of axon growth by myosin II–dependent mechanocatalysis of cofilin activity
Zhang XF, Ajeti V, Tsai N, Fereydooni A, Burns W, Murrell M, De La Cruz EM, Forscher P. Regulation of axon growth by myosin II–dependent mechanocatalysis of cofilin activity. Journal Of Cell Biology 2019, 218: 2329-2349. PMID: 31123185, PMCID: PMC6605792, DOI: 10.1083/jcb.201810054.Peer-Reviewed Original Research
2016
Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation
Buck KB, Schaefer AW, Schoonderwoert VT, Creamer MS, Dufresne ER, Forscher P. Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation. Molecular Biology Of The Cell 2016, 28: 98-110. PMID: 27852899, PMCID: PMC5221634, DOI: 10.1091/mbc.e16-04-0228.Peer-Reviewed Original ResearchKv3.3 Channels Bind Hax-1 and Arp2/3 to Assemble a Stable Local Actin Network that Regulates Channel Gating
Zhang Y, Zhang XF, Fleming MR, Amiri A, El-Hassar L, Surguchev AA, Hyland C, Jenkins DP, Desai R, Brown MR, Gazula VR, Waters MF, Large CH, Horvath TL, Navaratnam D, Vaccarino FM, Forscher P, Kaczmarek LK. Kv3.3 Channels Bind Hax-1 and Arp2/3 to Assemble a Stable Local Actin Network that Regulates Channel Gating. Cell 2016, 165: 434-448. PMID: 26997484, PMCID: PMC4826296, DOI: 10.1016/j.cell.2016.02.009.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin-Related Protein 2Actin-Related Protein 2-3 ComplexActin-Related Protein 3Adaptor Proteins, Signal TransducingAmino Acid SequenceCell MembraneMolecular Sequence DataMutationNeuronsPluripotent Stem CellsRac GTP-Binding ProteinsShaw Potassium ChannelsSignal TransductionSpinocerebellar AtaxiasConceptsCytoplasmic C-terminusProline-rich domainPlasma membraneHAX-1Actin nucleationC-terminusCortical actin filament networkLocal actin networkStem cell-derived neuronsActin filament networkCell-derived neuronsAnti-apoptotic proteinsActin cytoskeletonKv3.3 potassium channelActin assemblyActin structuresActin networkArp2/3Channel gatingFilament networkGrowth conesCerebellar neurodegenerationKv3.3TerminusPotassium channels
2014
Regeneration of Aplysia Bag Cell Neurons is Synergistically Enhanced by Substrate-Bound Hemolymph Proteins and Laminin
Hyland C, Dufresne ER, Forscher P. Regeneration of Aplysia Bag Cell Neurons is Synergistically Enhanced by Substrate-Bound Hemolymph Proteins and Laminin. Scientific Reports 2014, 4: 4617. PMID: 24722588, PMCID: PMC3983596, DOI: 10.1038/srep04617.Peer-Reviewed Original ResearchConceptsBag cell neuronsHemolymph proteinsRespiratory protein hemocyaninAplysia bag cell neuronsProtein complexesFurther molecular characterizationAddition of hemolymphHigh molecular weight proteinsCell neuronsMolecular weight proteinsMolecular characterizationCellular targetsExtracellular matrixProteinNervous system repairNovel synergistic effectWeight proteinsLaminin substrateHumoral proteinsLamininPossible cooperationActive factorsMigration rateEndogenous factorsPotential relevance
2013
Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
Yang Q, Zhang XF, Van Goor D, Dunn AP, Hyland C, Medeiros N, Forscher P. Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones. Molecular Biology Of The Cell 2013, 24: 3097-3114. PMID: 23966465, PMCID: PMC3784383, DOI: 10.1091/mbc.e13-05-0289.Peer-Reviewed Original ResearchConceptsProtein kinase CMyosin II contractilityActin network densityNeuronal growth conesPKC activationCentral cytoplasmic domainRetrograde actin network flowTwo-tiered mechanismEffect of PKCActin network flowActin network structureActin filament networkGrowth conesProtein kinase C activationKinase C activationCytoplasmic domainActin polymerizationKinase CFilament networkCytoskeletal mechanismsRegulatory light chain phosphorylationPKC actionPKC activityC activationGuidance responses
2012
Calcineurin-dependent cofilin activation and increased retrograde actin flow drive 5-HT–dependent neurite outgrowth in Aplysia bag cell neurons
Zhang XF, Hyland C, Van Goor D, Forscher P. Calcineurin-dependent cofilin activation and increased retrograde actin flow drive 5-HT–dependent neurite outgrowth in Aplysia bag cell neurons. Molecular Biology Of The Cell 2012, 23: 4833-4848. PMID: 23097492, PMCID: PMC3521690, DOI: 10.1091/mbc.e12-10-0715.Peer-Reviewed Original ResearchConceptsPhospholipase CNeurite outgrowthDynamic cytoskeletal processesRetrograde actin network flowP domainRetrograde actin flowActin network flowSoluble growth factorsAplysia bag cell neuronsBag cell neuronsCofilin activityWidespread mechanismCytoskeletal processesActin flowCofilin activationCell neuronsNeurite outgrowth rateMechanistic roleInositol trisphosphateOutgrowthGrowth factorDirect activationOutgrowth rateBasal levelsActivation
2008
Myosin II Activity Facilitates Microtubule Bundling in the Neuronal Growth Cone Neck
Burnette DT, Ji L, Schaefer AW, Medeiros NA, Danuser G, Forscher P. Myosin II Activity Facilitates Microtubule Bundling in the Neuronal Growth Cone Neck. Developmental Cell 2008, 15: 163-169. PMID: 18606149, PMCID: PMC2548298, DOI: 10.1016/j.devcel.2008.05.016.Peer-Reviewed Original ResearchCoordination of Actin Filament and Microtubule Dynamics during Neurite Outgrowth
Schaefer AW, Schoonderwoert VT, Ji L, Mederios N, Danuser G, Forscher P. Coordination of Actin Filament and Microtubule Dynamics during Neurite Outgrowth. Developmental Cell 2008, 15: 146-162. PMID: 18606148, PMCID: PMC2595147, DOI: 10.1016/j.devcel.2008.05.003.Peer-Reviewed Original ResearchConceptsRetrograde actin network flowActin network flowNeurite outgrowthMyosin II contractilityRho-kinaseCell adhesion substratesCytoskeletal dynamicsMicrotubule behaviorAdhesion sitesAdhesion substrateMicrotubule dynamicsMyosin IIActin filamentsMolecular eventsGrowth responseMicrotubule movementKinaseNeuronal growthMicrotubulesOutgrowthGrowth cone neckGrowthOrganellesRegulationCascade
2006
Myosin II functions in actin-bundle turnover in neuronal growth cones
Medeiros NA, Burnette DT, Forscher P. Myosin II functions in actin-bundle turnover in neuronal growth cones. Nature Cell Biology 2006, 8: 216-226. PMID: 16501565, DOI: 10.1038/ncb1367.Peer-Reviewed Original Research
2001
Protein Kinase C Activation Promotes Microtubule Advance in Neuronal Growth Cones by Increasing Average Microtubule Growth Lifetimes
Kabir N, Schaefer A, Nakhost A, Sossin W, Forscher P. Protein Kinase C Activation Promotes Microtubule Advance in Neuronal Growth Cones by Increasing Average Microtubule Growth Lifetimes. Journal Of Cell Biology 2001, 152: 1033-1044. PMID: 11238458, PMCID: PMC2198821, DOI: 10.1083/jcb.152.5.1033.Peer-Reviewed Original Research
2000
Substrate–cytoskeletal coupling as a mechanism for the regulation of growth cone motility and guidance
Suter D, Forscher P. Substrate–cytoskeletal coupling as a mechanism for the regulation of growth cone motility and guidance. Developmental Neurobiology 2000, 44: 97-113. PMID: 10934315, DOI: 10.1002/1097-4695(200008)44:2<97::aid-neu2>3.0.co;2-u.Peer-Reviewed Original ResearchConceptsGrowth cone motilityCone motilityGuidance cuesGrowth conesDifferent guidance cuesDynamic cytoskeletonCell adhesion moleculeSignal transducerAxon guidanceMolecular componentsCytoskeletonMotile structuresMotility deviceAppropriate target cellsDifferent functionsRespective receptorsAdhesion moleculesProteinAxonal growthMotilityGrowth cone movementTarget cellsNeuronal processesRecent evidenceCone movementLocalization of unconventional myosins V and VI in neuronal growth cones
Suter D, Espindola F, Lin C, Forscher P, Mooseker M. Localization of unconventional myosins V and VI in neuronal growth cones. Developmental Neurobiology 2000, 42: 370-382. PMID: 10645976, DOI: 10.1002/(sici)1097-4695(20000215)42:3<370::aid-neu8>3.0.co;2-v.Peer-Reviewed Original Research
1999
A diffusion barrier maintains distribution of membrane proteins in polarized neurons
Winckler B, Forscher P, Mellman I. A diffusion barrier maintains distribution of membrane proteins in polarized neurons. Nature 1999, 397: 698-701. PMID: 10067893, DOI: 10.1038/17806.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAxonsBiological TransportCell CompartmentationCell MembraneCell PolarityCells, CulturedCytoskeletonDiffusionDimethyl SulfoxideLeukocyte L1 Antigen ComplexMembrane GlycoproteinsMembrane ProteinsMicrospheresNeural Cell Adhesion MoleculesNeuronsRatsReceptors, AMPAThy-1 AntigensConceptsMembrane proteinsDiffusion barrierObvious physical barriersPlasma membrane domainsLateral mobilityOptical tweezersCell-cell contactMembrane domainsPresumptive domainPolarized neuronsPlasma membraneCytoskeletal componentsPolarized distributionF-actinDiffusion of proteinsDistinct domainsBasolateral surfaceMembrane markersProteinSpecialized domainsInitial segmentTight junctionsAsymmetric distributionPhysical barrierTweezers
1998
Binding of Protein Kinase C Isoforms to Actin in Aplysia
Nakhost A, Forscher P, Sossin W. Binding of Protein Kinase C Isoforms to Actin in Aplysia. Journal Of Neurochemistry 1998, 71: 1221-1231. PMID: 9721748, DOI: 10.1046/j.1471-4159.1998.71031221.x.Peer-Reviewed Original ResearchConceptsPKC Apl IIProtein kinase C isoformsActin filamentsTriton-insoluble fractionApl IIF-actinCytoskeletal dynamicsC isoformsPhorbol ester-dependent mannerPKC Apl IMarine mollusk Aplysia californicaPhorbol ester treatmentNeuronal growth conesMollusk Aplysia californicaPKC phosphorylationApl IPKC-epsilonPhysiological roleIndependent PKCStriking colocalizationPKC inhibitorKinasePKC betaIIEster treatmentPKCThe Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate–Cytoskeletal Coupling
Suter D, Errante L, Belotserkovsky V, Forscher P. The Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate–Cytoskeletal Coupling. Journal Of Cell Biology 1998, 141: 227-240. PMID: 9531561, PMCID: PMC2132711, DOI: 10.1083/jcb.141.1.227.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAplysiaCell Adhesion MoleculesCell MovementCells, CulturedCloning, MolecularCytoskeletonDNA PrimersGanglia, InvertebrateImmunoglobulin GKineticsMicroscopy, VideoMicrotubulesModels, BiologicalNeuronsPolymerase Chain ReactionRecombinant ProteinsSilicon DioxideStress, MechanicalTubulin
1994
Cytoskeletal reorganization underlying growth cone motility
Lin C, Thompson C, Forscher P. Cytoskeletal reorganization underlying growth cone motility. Current Opinion In Neurobiology 1994, 4: 640-647. PMID: 7849519, DOI: 10.1016/0959-4388(94)90004-3.Peer-Reviewed Original ResearchConceptsIndividual cytoskeletal elementsGrowth cone motilityCytoskeletal dynamicsCytoskeletal reorganizationIntracellular cytoskeletonExtracellular substratesCytoskeletal elementsLiving cellsCone motilityNeuronal migrationNeuronal outgrowthFunctional couplingRecent studiesCytoskeletonImportant componentRegulation
1992
Novel form of growth cone motility involving site-directed actin filament assembly
Forscher P, Lin C, Thompson C. Novel form of growth cone motility involving site-directed actin filament assembly. Nature 1992, 357: 515-518. PMID: 1608453, DOI: 10.1038/357515a0.Peer-Reviewed Original ResearchConceptsActin filament assemblyFilament assemblyNeuronal growth conesCortical F-actin networkMembrane-cytoskeletal interfaceF-actin assemblyF-actin networkGrowth conesGrowth cone migrationGrowth cone motilityExtracellular signalsBacterial parasiteMembrane proteinsCell movementCytoskeletal structuresIntracellular movementDevelopmental processesBead movementCell locomotionCone migrationMorphogenic changesMotile cellsAxonal guidanceCone motilityCellular mechanisms
1989
Calcium and polyphosphoinositide control of cytoskeletal dynamics
Forscher P. Calcium and polyphosphoinositide control of cytoskeletal dynamics. Trends In Neurosciences 1989, 12: 468-474. PMID: 2479150, DOI: 10.1016/0166-2236(89)90098-2.Peer-Reviewed Original ResearchConceptsMajor actin-binding proteinActin-binding proteinsNeuronal cell typesCytoskeletal dynamicsCytoskeletal plasticityCytoskeletal proteinsRegulatory proteinsActin polymerizationNeuronal motilityActin filamentsMembrane interactionsCell typesNeuronal cytoskeletonProteinReceptor-mediated changesPolyphosphoinositide turnoverSynaptic functionDynamic alterationsMechanochemical propertiesAxonal growthPhosphoinositide turnoverCell structureRecent evidenceTurnoverIntracellular calcium