2024
Piwi regulates the usage of alternative transcription start sites in the Drosophila ovary
Chen J, Liu N, Qi H, Neuenkirchen N, Huang Y, Lin H. Piwi regulates the usage of alternative transcription start sites in the Drosophila ovary. Nucleic Acids Research 2024, gkae1160. PMID: 39657757, DOI: 10.1093/nar/gkae1160.Peer-Reviewed Original ResearchTranscription start siteTranscription start site usageFacilitates chromatin transcriptionDrosophila ovaryOvarian somatic cellsStart siteCap analysis of gene expression sequencingAlternative transcription start sitesSomatic cellsPol II initiationRNA polymerase IIAlternative transcription initiationRNA-binding proteinsCultured ovarian somatic cellsGene expression sequencingMRNA elongationPolymerase IIPol IITranscription initiationChromatin transcriptionTranscriptional regulationExpressed sequencesMutant ovariesPIWICap analysis
2021
Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries
Liu N, Neuenkirchen N, Zhong M, Lin H. Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries. G3: Genes, Genomes, Genetics 2021, 11: jkaa059. PMID: 33609367, PMCID: PMC8022938, DOI: 10.1093/g3journal/jkaa059.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsProtein-coding genesDrosophila ovaryGermline stem cell maintenanceRole of piRNAsTermination sitesGenome-wide mappingGenomic binding profileTranscriptional termination sitesSpecific genomic sitesStem cell maintenanceRNA pathwaysTransposon repressionTranscriptional startEuchromatic regionsGene regulationEpigenetic regulationGenomic sitesCell maintenancePiwiSpecific lociMethylation signalsDiverse mechanismsTarget siteBinding sites