2000
Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552′phoA In Vitro Transposition System
Braunstein M, Griffin T, Kriakov J, Friedman S, Grindley N, Jacobs W. Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552′phoA In Vitro Transposition System. Journal Of Bacteriology 2000, 182: 2732-2740. PMID: 10781540, PMCID: PMC101980, DOI: 10.1128/jb.182.10.2732-2740.2000.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAmino Acid SequenceArtificial Gene FusionBacterial ProteinsBiological TransportCosmidsCyclin-Dependent KinasesDNA Transposable ElementsDNA, BacterialGenes, BacterialGenomic LibraryMolecular Sequence DataMutagenesis, InsertionalMycobacterium tuberculosisPlasmidsReplication OriginConceptsProtective immunityM. tuberculosisMycobacterium tuberculosis pathogenesisImmune responseTuberculosis pathogenesisMycobacterium tuberculosis proteinsM. tuberculosis databaseTuberculosisTuberculosis databasePathogenesisTuberculosis proteinsImmunityEnvelope-associated proteinsM. smegmatisProteinPeptides
1985
Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli
Joyce C, Fujii D, Laks H, Hughes C, Grindley N. Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli. Journal Of Molecular Biology 1985, 186: 283-293. PMID: 3910840, DOI: 10.1016/0022-2836(85)90105-6.Peer-Reviewed Original ResearchConceptsDNA sequence analysisDNA polymerase IThree-dimensional structurePolymerase ISequence analysisPolA geneSingle-subunit enzymeEscherichia coliEnzyme-DNA interactionsGenetic mappingDeletion mutantsSubunit enzymeMutant formsPrimary sequenceMutational changesBacteriophage lambdaExcellent modelPolA mutantsPolA mutationEnzymatic behaviorMutantsMutationsGenesDNAColi
1984
Method for determining whether a gene of Escherichia coli is essential: application to the polA gene
Joyce C, Grindley N. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. Journal Of Bacteriology 1984, 158: 636-643. PMID: 6233260, PMCID: PMC215477, DOI: 10.1128/jb.158.2.636-643.1984.Peer-Reviewed Original ResearchConceptsPolA geneTarget genesRich mediumEscherichia coliPhage lambda vectorTarget lociBacterial chromosomeHomologous recombinationPhysical mapProphage excisionDNA segmentsMinimal mediumAntibiotic resistance genesPolymerase 3Recombinational eventsLambda vectorGenesResistance genesChromosomal deletionsDeletionFunctional fragmentsExonucleaseMarked deletionPhagesPresence of plasmids
1982
Identification of two genes immediately downstream from the polA gene of Escherichia coli
Joyce C, Grindley N. Identification of two genes immediately downstream from the polA gene of Escherichia coli. Journal Of Bacteriology 1982, 152: 1211-1219. PMID: 6183253, PMCID: PMC221628, DOI: 10.1128/jb.152.3.1211-1219.1982.Peer-Reviewed Original Research