2004
The Left End of IS2: a Compromise between Transpositional Activity and an Essential Promoter Function That Regulates the Transposition Pathway
Lewis L, Cylin E, Lee H, Saby R, Wong W, Grindley N. The Left End of IS2: a Compromise between Transpositional Activity and an Essential Promoter Function That Regulates the Transposition Pathway. Journal Of Bacteriology 2004, 186: 858-865. PMID: 14729714, PMCID: PMC321474, DOI: 10.1128/jb.186.3.858-865.2004.Peer-Reviewed Original Research
2003
The Architecture of the γδ Resolvase Crossover Site Synaptic Complex Revealed by Using Constrained DNA Substrates
Leschziner A, Grindley N. The Architecture of the γδ Resolvase Crossover Site Synaptic Complex Revealed by Using Constrained DNA Substrates. Molecular Cell 2003, 12: 775-781. PMID: 14527421, DOI: 10.1016/s1097-2765(03)00351-4.Peer-Reviewed Original Research
2001
A Model for the γδ Resolvase Synaptic Complex
Sarkis G, Murley L, Leschziner A, Boocock M, Stark W, Grindley N. A Model for the γδ Resolvase Synaptic Complex. Molecular Cell 2001, 8: 623-631. PMID: 11583624, DOI: 10.1016/s1097-2765(01)00334-3.Peer-Reviewed Original Research
1999
In vitro transposition of Tn552: A tool for DNA sequencing and mutagenesis
Griffin T, Leschziner A, Grindley N, Parsons L, DeVost J, Derbyshire K. In vitro transposition of Tn552: A tool for DNA sequencing and mutagenesis. Nucleic Acids Research 1999, 27: 3859-3865. PMID: 10481025, PMCID: PMC148649, DOI: 10.1093/nar/27.19.3859.Peer-Reviewed Original ResearchConceptsDNA sequencingTarget DNAEntire operonInsertion mutantsGene clusterGenetic toolsNucleotide sequenceSequence specificityUnique sequencesMolecular biologyTransposition reactionNovel transposonTransposonSequencingComprehensive collectionDNAArsenal of toolsSequenceOperonGenomicsMutantsCosmidsMutagenesisKbBiologyMutants of Tn3 resolvase which do not require accessory binding sites for recombination activity
Arnold P, Blake D, Grindley N, Boocock M, Stark W. Mutants of Tn3 resolvase which do not require accessory binding sites for recombination activity. The EMBO Journal 1999, 18: 1407-1414. PMID: 10064606, PMCID: PMC1171230, DOI: 10.1093/emboj/18.5.1407.Peer-Reviewed Original Research
1998
Architecture of the γδ Resolvase Synaptosome Oriented Heterodimers Identify Interactions Essential for Synapsis and Recombination
Murley L, Grindley N. Architecture of the γδ Resolvase Synaptosome Oriented Heterodimers Identify Interactions Essential for Synapsis and Recombination. Cell 1998, 95: 553-562. PMID: 9827807, DOI: 10.1016/s0092-8674(00)81622-0.Peer-Reviewed Original ResearchTn552 transposase catalyzes concerted strand transfer in vitro
Leschziner A, Griffin T, Grindley N. Tn552 transposase catalyzes concerted strand transfer in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 7345-7350. PMID: 9636151, PMCID: PMC22612, DOI: 10.1073/pnas.95.13.7345.Peer-Reviewed Original Research
1997
Two abundant intramolecular transposition products, resulting from reactions initiated at a single end, suggest that IS2 transposes by an unconventional pathway
Lewis L, Grindley N. Two abundant intramolecular transposition products, resulting from reactions initiated at a single end, suggest that IS2 transposes by an unconventional pathway. Molecular Microbiology 1997, 25: 517-529. PMID: 9302014, DOI: 10.1046/j.1365-2958.1997.4871848.x.Peer-Reviewed Original ResearchBacterial ProteinsBase SequenceBinding SitesCloning, MolecularDNA NucleotidyltransferasesDNA PrimersDNA Transposable ElementsDNA, BacterialDNA, CircularEscherichia coliEscherichia coli ProteinsMicroscopy, ElectronModels, GeneticMolecular Sequence DataNucleic Acid ConformationPolymerase Chain ReactionRecombinant Fusion ProteinsTransposases
1996
Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation
Derbyshire K, Grindley N. Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation. Molecular Microbiology 1996, 21: 1261-1272. PMID: 8898394, DOI: 10.1111/j.1365-2958.1996.tb02587.x.Peer-Reviewed Original ResearchConceptsClasses of mutationsLevel of transpositionDNA-binding proteinsCis-acting proteinsAmount of transposaseCis preferenceWild-type transposaseInefficient translation initiationSite of synthesisAmino acids 25Translation initiationTranslational initiationTransposase proteinTranslation efficiencyMutant geneGene expressionProtein instabilityTransposase geneInefficient translationProline substitutionTransposaseMutant transposaseMutationsProteinUnusual class
1995
DNA transposition: From a black box to a color monitor
Grindley N, Leschziner A. DNA transposition: From a black box to a color monitor. Cell 1995, 83: 1063-1066. PMID: 8548793, DOI: 10.1016/0092-8674(95)90132-9.Peer-Reviewed Original ResearchCatalytic residues of gamma delta resolvase act in cis.
Boocock M, Zhu X, Grindley N. Catalytic residues of gamma delta resolvase act in cis. The EMBO Journal 1995, 14: 5129-5140. PMID: 7588641, PMCID: PMC394616, DOI: 10.1002/j.1460-2075.1995.tb00195.x.Peer-Reviewed Original ResearchBase SequenceBinding SitesCrossing Over, GeneticDNA NucleotidyltransferasesDNA Topoisomerases, Type IDNA Transposable ElementsGenetic Complementation TestModels, GeneticModels, MolecularMolecular Sequence DataPlasmidsRecombination, GeneticStructure-Activity RelationshipSubstrate SpecificityTransposasesA functional analysis of the inverted repeat of the gamma delta transposable element.
May E, Grindley N. A functional analysis of the inverted repeat of the gamma delta transposable element. Journal Of Molecular Biology 1995, 247: 578-87. PMID: 7723015, DOI: 10.1006/jmbi.1995.0164.Peer-Reviewed Original ResearchConceptsIntegration host factorInverted repeatsBase pairsTransposable elementsTransposase bindingGroove contactsIHF siteReduced transposition activityTerminal inverted repeatsMinor groove contactsBase pair regionGamma delta transposaseBase pair stretchSusceptible to mutationsTransposon gamma deltaTn3 familyTransposition activityPoint mutantsTarget plasmidTransposition defectBinding regionMutationsBinding sitesBinding contactsHost factorsA functional analysis of the inverted repeat of the γδ transposable element
May E, Grindley N. A functional analysis of the inverted repeat of the γδ transposable element. Journal Of Molecular Biology 1995, 247: 578-587. DOI: 10.1016/s0022-2836(05)80139-1.Peer-Reviewed Original ResearchThe tyrosine‐6 hydroxyl of γδ resolvase is not required for the DNA cleavage and rejoining reactions
Leschziner A, Boocock M, Grindley N. The tyrosine‐6 hydroxyl of γδ resolvase is not required for the DNA cleavage and rejoining reactions. Molecular Microbiology 1995, 15: 865-870. PMID: 7596288, DOI: 10.1111/j.1365-2958.1995.tb02356.x.Peer-Reviewed Original Research
1993
Analysis of a Nucleoprotein Complex: the Synaptosome of γδ Resolvase
Grindley N. Analysis of a Nucleoprotein Complex: the Synaptosome of γδ Resolvase. Science 1993, 262: 738-740. PMID: 8235593, DOI: 10.1126/science.8235593.Peer-Reviewed Original ResearchProtein‐protein interactions directing resolvase site‐specific recombination: a structure‐function analysis.
Hughes R, Rice P, Steitz T, Grindley N. Protein‐protein interactions directing resolvase site‐specific recombination: a structure‐function analysis. The EMBO Journal 1993, 12: 1447-1458. PMID: 8385604, PMCID: PMC413356, DOI: 10.1002/j.1460-2075.1993.tb05788.x.Peer-Reviewed Original ResearchMapping interactions between the catalytic domain of resolvase and its DNA substrate using cysteine-coupled EDTA-iron.
Mazzarelli J, Ermácora M, Fox R, Grindley N. Mapping interactions between the catalytic domain of resolvase and its DNA substrate using cysteine-coupled EDTA-iron. Biochemistry 1993, 32: 2979-86. PMID: 8384484, DOI: 10.1021/bi00063a008.Peer-Reviewed Original Research
1992
Binding of the IS903 transposase to its inverted repeat in vitro.
Derbyshire K, Grindley N. Binding of the IS903 transposase to its inverted repeat in vitro. The EMBO Journal 1992, 11: 3449-3455. PMID: 1324175, PMCID: PMC556880, DOI: 10.1002/j.1460-2075.1992.tb05424.x.Peer-Reviewed Original Research
1991
Resolvase‐catalysed reactions between res sites differing in the central dinucleotide of subsite I.
Stark W, Grindley N, Hatfull G, Boocock M. Resolvase‐catalysed reactions between res sites differing in the central dinucleotide of subsite I. The EMBO Journal 1991, 10: 3541-3548. PMID: 1655422, PMCID: PMC453083, DOI: 10.1002/j.1460-2075.1991.tb04918.x.Peer-Reviewed Original ResearchGamma delta transposase. Purification and analysis of its interaction with a transposon end
Wiater L, Grindley N. Gamma delta transposase. Purification and analysis of its interaction with a transposon end. Journal Of Biological Chemistry 1991, 266: 1841-1849. PMID: 1846366, DOI: 10.1016/s0021-9258(18)52370-x.Peer-Reviewed Original Research