2006
Implications of structures of synaptic tetramers of γδ resolvase for the mechanism of recombination
Kamtekar S, Ho RS, Cocco MJ, Li W, Wenwieser SV, Boocock MR, Grindley ND, Steitz TA. Implications of structures of synaptic tetramers of γδ resolvase for the mechanism of recombination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 10642-10647. PMID: 16807292, PMCID: PMC1483221, DOI: 10.1073/pnas.0604062103.Peer-Reviewed Original ResearchMechanisms of Site-Specific Recombination*
Grindley ND, Whiteson KL, Rice PA. Mechanisms of Site-Specific Recombination*. Annual Review Of Biochemistry 2006, 75: 567-605. PMID: 16756503, DOI: 10.1146/annurev.biochem.73.011303.073908.Peer-Reviewed Original Research
2005
Structure of a Synaptic γδ Resolvase Tetramer Covalently Linked to Two Cleaved DNAs
Li W, Kamtekar S, Xiong Y, Sarkis GJ, Grindley ND, Steitz TA. Structure of a Synaptic γδ Resolvase Tetramer Covalently Linked to Two Cleaved DNAs. Science 2005, 309: 1210-1215. PMID: 15994378, DOI: 10.1126/science.1112064.Peer-Reviewed Original Research
2003
The Architecture of the γδ Resolvase Crossover Site Synaptic Complex Revealed by Using Constrained DNA Substrates
Leschziner A, Grindley N. The Architecture of the γδ Resolvase Crossover Site Synaptic Complex Revealed by Using Constrained DNA Substrates. Molecular Cell 2003, 12: 775-781. PMID: 14527421, DOI: 10.1016/s1097-2765(03)00351-4.Peer-Reviewed Original Research
2001
A Model for the γδ Resolvase Synaptic Complex
Sarkis G, Murley L, Leschziner A, Boocock M, Stark W, Grindley N. A Model for the γδ Resolvase Synaptic Complex. Molecular Cell 2001, 8: 623-631. PMID: 11583624, DOI: 10.1016/s1097-2765(01)00334-3.Peer-Reviewed Original Research
1999
Mutants of Tn3 resolvase which do not require accessory binding sites for recombination activity
Arnold P, Blake D, Grindley N, Boocock M, Stark W. Mutants of Tn3 resolvase which do not require accessory binding sites for recombination activity. The EMBO Journal 1999, 18: 1407-1414. PMID: 10064606, PMCID: PMC1171230, DOI: 10.1093/emboj/18.5.1407.Peer-Reviewed Original Research
1998
Architecture of the γδ Resolvase Synaptosome Oriented Heterodimers Identify Interactions Essential for Synapsis and Recombination
Murley L, Grindley N. Architecture of the γδ Resolvase Synaptosome Oriented Heterodimers Identify Interactions Essential for Synapsis and Recombination. Cell 1998, 95: 553-562. PMID: 9827807, DOI: 10.1016/s0092-8674(00)81622-0.Peer-Reviewed Original Research
1997
Site-specific recombination: Synapsis and strand exchange revealed
Grindley N. Site-specific recombination: Synapsis and strand exchange revealed. Current Biology 1997, 7: r608-r612. PMID: 9368738, DOI: 10.1016/s0960-9822(06)00314-9.Peer-Reviewed Original Research
1995
Catalytic residues of gamma delta resolvase act in cis.
Boocock M, Zhu X, Grindley N. Catalytic residues of gamma delta resolvase act in cis. The EMBO Journal 1995, 14: 5129-5140. PMID: 7588641, PMCID: PMC394616, DOI: 10.1002/j.1460-2075.1995.tb00195.x.Peer-Reviewed Original ResearchBase SequenceBinding SitesCrossing Over, GeneticDNA NucleotidyltransferasesDNA Topoisomerases, Type IDNA Transposable ElementsGenetic Complementation TestModels, GeneticModels, MolecularMolecular Sequence DataPlasmidsRecombination, GeneticStructure-Activity RelationshipSubstrate SpecificityTransposasesThe tyrosine‐6 hydroxyl of γδ resolvase is not required for the DNA cleavage and rejoining reactions
Leschziner A, Boocock M, Grindley N. The tyrosine‐6 hydroxyl of γδ resolvase is not required for the DNA cleavage and rejoining reactions. Molecular Microbiology 1995, 15: 865-870. PMID: 7596288, DOI: 10.1111/j.1365-2958.1995.tb02356.x.Peer-Reviewed Original Research
1993
Protein‐protein interactions directing resolvase site‐specific recombination: a structure‐function analysis.
Hughes R, Rice P, Steitz T, Grindley N. Protein‐protein interactions directing resolvase site‐specific recombination: a structure‐function analysis. The EMBO Journal 1993, 12: 1447-1458. PMID: 8385604, PMCID: PMC413356, DOI: 10.1002/j.1460-2075.1993.tb05788.x.Peer-Reviewed Original Research
1991
Resolvase‐catalysed reactions between res sites differing in the central dinucleotide of subsite I.
Stark W, Grindley N, Hatfull G, Boocock M. Resolvase‐catalysed reactions between res sites differing in the central dinucleotide of subsite I. The EMBO Journal 1991, 10: 3541-3548. PMID: 1655422, PMCID: PMC453083, DOI: 10.1002/j.1460-2075.1991.tb04918.x.Peer-Reviewed Original Research
1990
Cooperativity mutants of the γδ resolvase identify an essential interdimer interaction
Hughes R, Hatfull G, Rice P, Steitz T, Grindley N. Cooperativity mutants of the γδ resolvase identify an essential interdimer interaction. Cell 1990, 63: 1331-1338. PMID: 2175679, DOI: 10.1016/0092-8674(90)90428-h.Peer-Reviewed Original ResearchConceptsProtein-protein interactionsHigher-order protein-protein interactionsCooperativity mutantsSite-specific recombinaseGamma delta resolvaseMutant phenotypeResolvase mutantsNucleoprotein complexesCrystallographic tetramersResolvase dimersΓδ resolvaseResolvaseCooperative bindingMutantsDNARecombinationSide chainsRecombinaseProteinInteractionCointegrate intermediatePhenotypeRecombination reactionBindingTetramerThe two functional domains of gamma delta resolvase act on the same recombination site: implications for the mechanism of strand exchange.
Dröge P, Hatfull G, Grindley N, Cozzarelli N. The two functional domains of gamma delta resolvase act on the same recombination site: implications for the mechanism of strand exchange. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 5336-5340. PMID: 2164677, PMCID: PMC54318, DOI: 10.1073/pnas.87.14.5336.Peer-Reviewed Original ResearchConceptsDNA-protein complexesRecombination sitesSite-specific recombinationGamma delta resolvaseDNA exchangeCatalytic domainStrand exchangeFunctional domainsResolvaseResolvase subunitsDNA strandsRes sitesSynaptic complexDNAStrand breakageRecombinationReunion eventDomainSitesComplexesSubunitsStrandsBreakageSynaptosomes
1988
The gamma delta resolvase bends the res site into a recombinogenic complex.
Salvo J, Grindley N. The gamma delta resolvase bends the res site into a recombinogenic complex. The EMBO Journal 1988, 7: 3609-3616. PMID: 2850169, PMCID: PMC454865, DOI: 10.1002/j.1460-2075.1988.tb03239.x.Peer-Reviewed Original ResearchUncoupling of the recombination and topoisomerase activities of the γδ resolvase by a mutation at the crossover point
Falvey E, Hatfull G, Grindley N. Uncoupling of the recombination and topoisomerase activities of the γδ resolvase by a mutation at the crossover point. Nature 1988, 332: 861-863. PMID: 2833710, DOI: 10.1038/332861a0.Peer-Reviewed Original Research
1987
Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats.
Derbyshire K, Hwang L, Grindley N. Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 8049-8053. PMID: 2825175, PMCID: PMC299474, DOI: 10.1073/pnas.84.22.8049.Peer-Reviewed Original ResearchThe γδ resolvase induces an unusual DNA structure at the recombinational crossover point
Hatfull G, Noble S, Grindley N. The γδ resolvase induces an unusual DNA structure at the recombinational crossover point. Cell 1987, 49: 103-110. PMID: 3030563, DOI: 10.1016/0092-8674(87)90760-4.Peer-Reviewed Original Research
1986
Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res.
Hatfull G, Grindley N. Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 5429-5433. PMID: 3016704, PMCID: PMC386300, DOI: 10.1073/pnas.83.15.5429.Peer-Reviewed Original ResearchConceptsSerine 10Resolvase mutantsRecombinational activitySite-specific recombination proteinsProtein-DNA complexesPosition 10Site-specific recombinationRecombination proteinsActive site serineMutant proteinsRecombinational crossover pointMutantsResolvaseRecombinational sitesLeucine changeSerineSite ICysteine changeGel electrophoresisSpecific defectsComplex formationCointegrate moleculeTransposon gamma deltaProteinDNA
1985
Transpositional Recombination in Prokaryotes
Grindley N, Reed R. Transpositional Recombination in Prokaryotes. Annual Review Of Biochemistry 1985, 54: 863-896. PMID: 2992361, DOI: 10.1146/annurev.bi.54.070185.004243.Peer-Reviewed Original Research