1995
A functional analysis of the inverted repeat of the gamma delta transposable element.
May E, Grindley N. A functional analysis of the inverted repeat of the gamma delta transposable element. Journal Of Molecular Biology 1995, 247: 578-87. PMID: 7723015, DOI: 10.1006/jmbi.1995.0164.Peer-Reviewed Original ResearchConceptsIntegration host factorInverted repeatsBase pairsTransposable elementsTransposase bindingGroove contactsIHF siteReduced transposition activityTerminal inverted repeatsMinor groove contactsBase pair regionGamma delta transposaseBase pair stretchSusceptible to mutationsTransposon gamma deltaTn3 familyTransposition activityPoint mutantsTarget plasmidTransposition defectBinding regionMutationsBinding sitesBinding contactsHost factors
1986
Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res.
Hatfull G, Grindley N. Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 5429-5433. PMID: 3016704, PMCID: PMC386300, DOI: 10.1073/pnas.83.15.5429.Peer-Reviewed Original ResearchConceptsSerine 10Resolvase mutantsRecombinational activitySite-specific recombination proteinsProtein-DNA complexesPosition 10Site-specific recombinationRecombination proteinsActive site serineMutant proteinsRecombinational crossover pointMutantsResolvaseRecombinational sitesLeucine changeSerineSite ICysteine changeGel electrophoresisSpecific defectsComplex formationCointegrate moleculeTransposon gamma deltaProteinDNA