1994
Resolvase-Mediated Site-Specific Recombination
Grindley N. Resolvase-Mediated Site-Specific Recombination. Nucleic Acids And Molecular Biology 1994, 8: 236-267. DOI: 10.1007/978-3-642-78666-2_14.Peer-Reviewed Original ResearchSite-specific recombinationConservative site-specific recombinationPlasmid chromosomesFunctional genesPhage genomeDNA segmentsSynthesis of DNABiological roleGene expressionChromosomal integrationMost biological systemsGene cassettesTransposition intermediatesReciprocal exchangeRecombinationBiological systemsGenomeChromosomesResolvaseGenesDNACassetteMonomerizationExpression
1983
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
Joyce C, Grindley N. Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 1830-1834. PMID: 6340110, PMCID: PMC393703, DOI: 10.1073/pnas.80.7.1830.Peer-Reviewed Original ResearchConceptsDNA polymerase IOverproducing strainPolymerase IGene fusion techniquesLarge proteolytic fragmentCellular proteinsLac promoterGene fragmentsProtein structurePhage lambdaLeftward promoterEscherichia coliCarboxyl terminalPolymerase fragmentProteolytic fragmentsKlenow fragmentPromoterPlasmidPurification procedureFragmentsOverproductionExpressionI. MoreoverMechanistic studiesCloning