2000
Regulation of DRA and AE1 in rat colon by dietary Na depletion
Rajendran V, Black J, Ardito T, Sangan P, Alper S, Schweinfest C, Kashgarian M, Binder H. Regulation of DRA and AE1 in rat colon by dietary Na depletion. AJP Gastrointestinal And Liver Physiology 2000, 279: g931-g942. PMID: 11052990, DOI: 10.1152/ajpgi.2000.279.5.g931.Peer-Reviewed Original Research
1999
Colonic H-K-ATPase β-subunit: identification in apical membranes and regulation by dietary K depletion
Sangan P, Kolla S, Rajendran V, Kashgarian M, Binder H. Colonic H-K-ATPase β-subunit: identification in apical membranes and regulation by dietary K depletion. American Journal Of Physiology 1999, 276: c350-c360. PMID: 9950762, DOI: 10.1152/ajpcell.1999.276.2.c350.Peer-Reviewed Original ResearchDifferential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon
Ikuma M, Kashgarian M, Binder H, Rajendran V. Differential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon. American Journal Of Physiology 1999, 276: g539-g549. PMID: 9950829, DOI: 10.1152/ajpgi.1999.276.2.g539.Peer-Reviewed Original ResearchConceptsDietary sodium depletionSodium depletionDistal colonRat colonProtein expressionMRNA abundanceBlot analysisDose-dependent inhibitorApical membrane vesiclesElectroneutral NaCl absorptionWestern blot analysisDifferential regulationNormal ratsProximal colonHOE 694Multiple diverse effectsColonDistal segmentsNaCl absorptionNHE isoformsExchange activityExchanger isoformsNorthern blot analysisDifferential effectsPretranslational level
1998
Role of heat stress response in the tolerance of immature renal tubules to anoxia
Gaudio K, Thulin G, Mann A, Kashgarian M, Siegel N. Role of heat stress response in the tolerance of immature renal tubules to anoxia. American Journal Of Physiology 1998, 274: f1029-f1036. PMID: 9841493, DOI: 10.1152/ajprenal.1998.274.6.f1029.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornBlotting, NorthernCarbonyl Cyanide m-Chlorophenyl HydrazoneCell HypoxiaDNA, MitochondrialDNA-Binding ProteinsElectrophoresisHeat Shock Transcription FactorsHeat-Shock ProteinsHot TemperatureHSP72 Heat-Shock ProteinsKidney TubulesMitochondriaNystatinOuabainOxygen ConsumptionRatsRNA, MessengerTranscription FactorsAntibodies against mesangial cells and their secretory products in chronic renal allograft rejection in the rat.
Paul L, Muralidharan J, Muzaffar S, Manting E, Valentin J, de Heer E, Kashgarian M. Antibodies against mesangial cells and their secretory products in chronic renal allograft rejection in the rat. American Journal Of Pathology 1998, 152: 1209-23. PMID: 9588890, PMCID: PMC1858589.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantibodiesAutoantigensBasement MembraneBiglycanBlotting, WesternCells, CulturedChronic DiseaseDecorinEndothelium, VascularExtracellular Matrix ProteinsFlow CytometryFluorescent Antibody Technique, IndirectGlomerular MesangiumGraft RejectionKidney TransplantationMaleProteoglycansRatsRats, Inbred F344Rats, Inbred LewTransplantation, HomologousConceptsMesangial cell culture supernatantsChronic renal allograft rejectionRenal allograft rejectionMesangial cellsChronic rejectionAllograft rejectionCell culture supernatantsChronic renal transplant rejectionFlow cytometryWestern blotEndothelial cellsGlomerular basement membrane antigenPost-transplant seraRenal transplant rejectionCell-mediated immunityPost-immunization seraPresence of antibodiesMesangial cell surfaceStrong autoantibody responseCulture supernatantsCultured mesangial cellsIndirect immunofluorescent studiesBasement membrane antigensIndirect immunofluorescent stainingTissue repair processATP releases HSP-72 from protein aggregates after renal ischemia
Aufricht C, Lu E, Thulin G, Kashgarian M, Siegel N, Van Why S. ATP releases HSP-72 from protein aggregates after renal ischemia. American Journal Of Physiology 1998, 274: f268-f274. PMID: 9486221, DOI: 10.1152/ajprenal.1998.274.2.f268.Peer-Reviewed Original ResearchHeat-shock protein 25 induction and redistribution during actin reorganization after renal ischemia
Aufricht C, Ardito T, Thulin G, Kashgarian M, Siegel N, Van Why S. Heat-shock protein 25 induction and redistribution during actin reorganization after renal ischemia. American Journal Of Physiology 1998, 274: f215-f222. PMID: 9458842, DOI: 10.1152/ajprenal.1998.274.1.f215.Peer-Reviewed Original ResearchConceptsHSP-25Renal ischemiaHeat shock protein 25Ischemic renal injuryH reflowRat renal cortexControl proximal tubulesRenal injuryPostischemic recoveryRenal cortexIschemiaProximal tubulesIntracytoplasmic accumulationPeak levelsHeat shock proteinsProtein 25Actin stainingInsoluble cytoskeletal fractionPunctate accumulationsCytoskeletal disruptionSmall heat shock proteinsRegulatory roleInductionIntracellular distributionCytoskeletal fraction
1997
Posttransplant antibody response and chronic rejection
Paul L, Valentin J, Muzaffar S, Kashgarian M. Posttransplant antibody response and chronic rejection. Transplantation Proceedings 1997, 29: 2529-2530. PMID: 9290727, DOI: 10.1016/s0041-1345(97)00493-4.Peer-Reviewed Original ResearchRegulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion
Sangan P, Rajendran V, Mann A, Kashgarian M, Binder H. Regulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion. American Journal Of Physiology 1997, 272: c685-c696. PMID: 9124313, DOI: 10.1152/ajpcell.1997.272.2.c685.Peer-Reviewed Original ResearchConceptsDietary K depletionDistal colonNA depletionK-ATPase activityProximal colonLarge intestineRat distal colonActive K absorptionK-ATPaseRenal collecting ductsDietary NaK-ATPase expressionK-ATPase isoformsK depletionRat colonCollecting ductsOuter medullaColonProtein expressionProtein levelsKidneyPolyclonal antibodiesIntestineDuctMRNA
1996
The α3 Isoform Protein of the Na+,K+-ATPase Is Associated With the Sites of Cardiac and Neuromuscular Impulse Transmission
Zahler R, Sun W, Ardito T, Zhang Z, Kocsis J, Kashgarian M. The α3 Isoform Protein of the Na+,K+-ATPase Is Associated With the Sites of Cardiac and Neuromuscular Impulse Transmission. Circulation Research 1996, 78: 870-879. PMID: 8620608, DOI: 10.1161/01.res.78.5.870.Peer-Reviewed Original ResearchConceptsNeuromuscular junctionConduction systemImpulse transmissionIsoform-specific antibodiesSkeletal muscle expressesSkeletal musclePresynaptic motor terminalsCardiac conduction systemHeart conduction systemAlpha subunitAxonal processesRat heartCardiac myocytesIsoform mRNAsMuscleClassic morphologyJunctional complexesImmunoreactive proteinPump isoformsAntibodiesIsoform proteinsHeartPump proteinImmunohistochemistryIsoformsNa-K-ATPase alpha-isoform expression in heart and vascular endothelia: cellular and developmental regulation
Zahler R, Sun W, Ardito T, Kashgarian M. Na-K-ATPase alpha-isoform expression in heart and vascular endothelia: cellular and developmental regulation. American Journal Of Physiology 1996, 270: c361-c371. PMID: 8772464, DOI: 10.1152/ajpcell.1996.270.1.c361.Peer-Reviewed Original ResearchConceptsCardiac conduction systemAlpha 2Alpha 1Alpha 3Vascular endotheliumConduction systemHigh ouabain affinityNa-K-ATPase isoformsDevelopmental regulationAlpha 3 expressionImportant species differencesNeonatal rat myocardiumIsoform patternNeonatal ratsPapillary musclesDistinct physiological rolesSmooth muscleImmunohistochemical analysisConduction tissueVentricular myocardiumOuabain affinityRat myocardiumNormal endotheliumRat heartT-tubule system
1995
Functional expression and segmental localization of rat colonic K-adenosine triphosphatase.
Lee J, Rajendran V, Mann A, Kashgarian M, Binder H. Functional expression and segmental localization of rat colonic K-adenosine triphosphatase. Journal Of Clinical Investigation 1995, 96: 2002-2008. PMID: 7560093, PMCID: PMC185838, DOI: 10.1172/jci118247.Peer-Reviewed Original ResearchPDGF and TGF-β mediate collagen production by mesangial cells exposed to advanced glycosylation end products
Throckmorton D, Brogden A, Min B, Rasmussen H, Kashgarian M. PDGF and TGF-β mediate collagen production by mesangial cells exposed to advanced glycosylation end products. Kidney International 1995, 48: 111-117. PMID: 7564067, DOI: 10.1038/ki.1995.274.Peer-Reviewed Original ResearchA unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator
Ameen N, Ardito T, Kashgarian M, Marino C. A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator. Gastroenterology 1995, 108: 1016-1023. PMID: 7535272, DOI: 10.1016/0016-5085(95)90198-1.Peer-Reviewed Original ResearchConceptsProximal small intestineCrypt epithelial cellsVillus cellsSmall intestineHuman proximal small intestineCystic fibrosis transmembrane conductance regulatorEpithelial cellsSurface marker expressionFibrosis transmembrane conductance regulatorBrush border sucraseTransmembrane conductance regulatorIntestinal villus cellsBACKGROUND/Marker antibodiesMarker expressionUnique subsetVillus enterocytesConductance regulatorIntestineLactase expressionBrush borderPostischemic thyroxin stimulates renal mitochondrial adenine nucleotide translocator activity.
Boydstun I, Najjar S, Kashgarian M, Carpenter T, Siegel N. Postischemic thyroxin stimulates renal mitochondrial adenine nucleotide translocator activity. American Journal Of Physiology 1995, 268: e651-6. PMID: 7733309, DOI: 10.1152/ajprenal.1995.268.4.f651.Peer-Reviewed Original Research
1994
Activation of heat-shock transcription factor by graded reductions in renal ATP, in vivo, in the rat.
Van Why S, Mann A, Thulin G, Zhu X, Kashgarian M, Siegel N. Activation of heat-shock transcription factor by graded reductions in renal ATP, in vivo, in the rat. Journal Of Clinical Investigation 1994, 94: 1518-1523. PMID: 7929828, PMCID: PMC295298, DOI: 10.1172/jci117492.Peer-Reviewed Original ResearchConceptsRenal cortexHsp-70 mRNA inductionCortical ATP levelsRenal ischemia resultsPartial aortic occlusionActivation of HSFATP levelsCellular ATPHSF activationHSP-70 mRNAReduction of ATPAortic occlusionPreocclusion levelsProfound fallTotal occlusionIschemia resultsPreocclusion valuesHeat shock transcription factorLow-level expressionMetabolic consequencesVivo 31P NMR spectroscopyControl valuesHSP-70Vigorous responseRenal ATPExpression and molecular regulation of Na(+)-K(+)-ATPase after renal ischemia
Van Why S, Mann A, Ardito T, Siegel N, Kashgarian M. Expression and molecular regulation of Na(+)-K(+)-ATPase after renal ischemia. American Journal Of Physiology 1994, 267: f75-f85. PMID: 8048568, DOI: 10.1152/ajprenal.1994.267.1.f75.Peer-Reviewed Original ResearchImmature Renal Tubules Are Resistant to Prolonged Anoxia
Gaudio K, Thulin G, Ardito T, Kashgarian M, Siegel N. Immature Renal Tubules Are Resistant to Prolonged Anoxia. Pediatric Research 1994, 35: 152-156. PMID: 8165048, DOI: 10.1203/00006450-199402000-00005.Peer-Reviewed Original ResearchThe Contribution of Mesangial Cell Collagen Synthesis to the Pathogenesis of Diabetic Nephropathy1
Kashgarian M, Oshima S, Takeuchi A, Throckmorton D, Rasmussen H. The Contribution of Mesangial Cell Collagen Synthesis to the Pathogenesis of Diabetic Nephropathy1. Contributions To Nephrology 1994, 107: 132-139. PMID: 8004959, DOI: 10.1159/000422971.Peer-Reviewed Original ResearchTransforming growth factor-beta 1 stimulates glomerular mesangial cell synthesis of the 72-kd type IV collagenase.
Marti H, Lee L, Kashgarian M, Lovett D. Transforming growth factor-beta 1 stimulates glomerular mesangial cell synthesis of the 72-kd type IV collagenase. American Journal Of Pathology 1994, 144: 82-94. PMID: 8291614, PMCID: PMC1887110.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCells, CulturedCollagenasesEnzyme-Linked Immunosorbent AssayGlomerular MesangiumHumansImmunohistochemistryMatrix Metalloproteinase 9Molecular ProbesMolecular Sequence DataNephritisRatsRats, WistarTranscription, GeneticTransforming Growth Factor betaConceptsType IV collagenaseHuman mesangial cellsType IVMesangial cellsImmune complex-mediated glomerulonephritisMRNA expressionType IV collagenase mRNA expressionCultured human mesangial cellsCollagenase mRNA expressionMesangial cell synthesisGlomerular inflammatory processesTIMP-1 synthesisNephritic ratsInflammatory processGlomerular matrixMesangial distributionMatrix protein synthesisTIMP-1Direct stimulationTissue inhibitorNormal animalsMesangial compartmentMatrix metalloproteinase synthesisStimulatory effectTIMP-1 inhibitor