2008
Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body
Chang W, Zaarour RF, Reck-Peterson S, Rinn J, Singer RH, Snyder M, Novick P, Mooseker MS. Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body. RNA 2008, 14: 491-502. PMID: 18218704, PMCID: PMC2248268, DOI: 10.1261/rna.665008.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdenosine TriphosphatasesBase SequenceDNA PrimersMacromolecular SubstancesMyosin Heavy ChainsMyosin Type VOligonucleotide Array Sequence AnalysisOrganellesPolyribosomesRibonucleoproteinsRNA Processing, Post-TranscriptionalRNA, FungalRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSecretory VesiclesVacuolesConceptsRNA processing bodiesClass V myosinsP-bodiesRelease of mRNAProcessing bodiesOrganelle traffickingSpindle orientationMotor mutantsMyo2-66Ribosomal subunitMyo2pProtein subunitsPartial colocalizationMicroarray analysisSubunitsSedimentation analysisYeastMRNAComplexesMyosinMutantsPolysomesTraffickingRNAColocalization
2006
Modulation of Cell Adhesion and Motility in the Immune System by Myo1f
Kim SV, Mehal WZ, Dong X, Heinrich V, Pypaert M, Mellman I, Dembo M, Mooseker MS, Wu D, Flavell RA. Modulation of Cell Adhesion and Motility in the Immune System by Myo1f. Science 2006, 314: 136-139. PMID: 17023661, DOI: 10.1126/science.1131920.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCD18 AntigensCell AdhesionCell DegranulationCell MovementChemotaxis, LeukocyteColony Count, MicrobialCytoplasmic GranulesExocytosisImmunity, InnateLigandsListeria monocytogenesListeriosisMiceMice, KnockoutMyosin Type INeutrophil ActivationNeutrophilsN-Formylmethionine Leucyl-Phenylalanine
2005
Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae
Shih J, Reck-Peterson S, Newitt R, Mooseker M, Aebersold R, Herskowitz I. Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae. Molecular Biology Of The Cell 2005, 16: 4595-4608. PMID: 16030260, PMCID: PMC1237067, DOI: 10.1091/mbc.e05-02-0108.Peer-Reviewed Original ResearchConceptsCell polarityPolarity proteinsActin functionCell wall morphogenesisCell polarity proteinsYeast cell polarityPresumptive bud siteCell separation defectATP-sensitive mannerTandem mass spectrometry analysisNonessential proteinsWall morphogenesisMolecular functionsBud sitePolarized localizationSpa2pMass spectrometry analysisSite of growthSaccharomyces cerevisiaeMyo2pCoimmunoprecipitation strategyCell cycleF-actinIndirect interactionsProtein
2003
Native Myosin-IXb is a plus-, not a minus-end-directed motor
O'Connell C, Mooseker M. Native Myosin-IXb is a plus-, not a minus-end-directed motor. Nature Cell Biology 2003, 5: 171-172. PMID: 12563277, DOI: 10.1038/ncb924.Peer-Reviewed Original Research
2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
2001
The Abl-related gene (Arg) nonreceptor tyrosine kinase uses two F-actin-binding domains to bundle F-actin
Wang Y, Miller A, Mooseker M, Koleske A. The Abl-related gene (Arg) nonreceptor tyrosine kinase uses two F-actin-binding domains to bundle F-actin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 14865-14870. PMID: 11752434, PMCID: PMC64950, DOI: 10.1073/pnas.251249298.Peer-Reviewed Original ResearchConceptsF-actin-binding domainActin-rich structuresAbl family kinasesNonreceptor tyrosine kinaseF-actinActin cytoskeletonFamily kinasesFluorescent protein fusion proteinTyrosine kinaseActin-bundling activityProtein fusion proteinActin cytoskeletal structuresCellular morphogenesisSwiss 3T3 fibroblastsBundling activityDeletion mutantsCytoskeletal structuresC-terminusFusion proteinFunctional interactionKinaseActin moleculesCytoskeletonPositive cooperativityArgHigh Affinity Binding of Brain Myosin-Va to F-actin Induced by Calcium in the Presence of ATP*
Tauhata S, dos Santos D, Taylor E, Mooseker M, Larson R. High Affinity Binding of Brain Myosin-Va to F-actin Induced by Calcium in the Presence of ATP*. Journal Of Biological Chemistry 2001, 276: 39812-39818. PMID: 11517216, DOI: 10.1074/jbc.m102583200.Peer-Reviewed Original ResearchThe Yeast Class V Myosins, Myo2p and Myo4p, Are Nonprocessive Actin-Based Motors
Reck-Peterson S, Tyska M, Novick P, Mooseker M. The Yeast Class V Myosins, Myo2p and Myo4p, Are Nonprocessive Actin-Based Motors. Journal Of Cell Biology 2001, 153: 1121-1126. PMID: 11381095, PMCID: PMC2174330, DOI: 10.1083/jcb.153.5.1121.Peer-Reviewed Original ResearchActinsAdenosine TriphosphateAnimalsAntibodiesBrainCalciumCalmodulin-Binding ProteinsCarrier ProteinsChickensFungal ProteinsKineticsMicroscopy, VideoMolecular Motor ProteinsMovementMyosin Heavy ChainsMyosin Type IIMyosin Type VMyosinsNerve Tissue ProteinsProtein BindingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe Proteins
2000
Myosin-V stepping kinetics: A molecular model for processivity
Rief M, Rock R, Mehta A, Mooseker M, Cheney R, Spudich J. Myosin-V stepping kinetics: A molecular model for processivity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 9482-9486. PMID: 10944217, PMCID: PMC16890, DOI: 10.1073/pnas.97.17.9482.Peer-Reviewed Original ResearchRole of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae
Karpova T, Reck-Peterson S, Elkind N, Mooseker M, Novick P, Cooper J. Role of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae. Molecular Biology Of The Cell 2000, 11: 1727-1737. PMID: 10793147, PMCID: PMC14879, DOI: 10.1091/mbc.11.5.1727.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCell DivisionCell PolarityFungal ProteinsGreen Fluorescent ProteinsLuminescent ProteinsMicroscopy, VideoMutationMyosin Heavy ChainsMyosin Type IIMyosin Type VRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsThiazolesThiazolidinesConceptsActin patch polarizationCell surface growthPolarized secretionFilamentous actinCortical actin patchesClass V myosin Myo2pRole of actinTime-lapse video microscopyIndividual living cellsActin patchesPolarized growthActin cytoskeletonActin cablesMyo2-66Tail domainMyo2pSevere defectsLiving cellsOfSaccharomyces cerevisiaeMotor domainLatrunculinActinOverall growthVideo microscopyQuantitative assayCompartmentalization of the Cell Cortex by Septins Is Required for Maintenance of Cell Polarity in Yeast
Barral Y, Mermall V, Mooseker M, Snyder M. Compartmentalization of the Cell Cortex by Septins Is Required for Maintenance of Cell Polarity in Yeast. Molecular Cell 2000, 5: 841-851. PMID: 10882120, DOI: 10.1016/s1097-2765(00)80324-x.Peer-Reviewed Original ResearchMeSH KeywordsActinsCarrier ProteinsCell CompartmentationCell Cycle ProteinsCell DivisionCell MembraneCell PolarityCytoplasmCytoskeletal ProteinsExocytosisFungal ProteinsMorphogenesisMyosin Heavy ChainsMyosin Type IIMyosin Type VProtein-Tyrosine KinasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsConceptsCell polaritySpecialized plasma membrane domainsIsotropic bud growthPlasma membrane domainsBud neckMembrane domainsMother cellsCell cortexCell peripheryGrowth polaritySeptinsProper regulationBud surfaceBiological processesBud growthCell polarizationIsotropic growthCortical domainsExocytosisPatch stabilityActive siteCellsMyo2Sec5SPA2
1999
An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin
Zhou D, Mooseker M, Galán J. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10176-10181. PMID: 10468582, PMCID: PMC17862, DOI: 10.1073/pnas.96.18.10176.Peer-Reviewed Original ResearchConceptsActin-bundling activityT-plastinBacterial-host cell contactHost cell signal transduction pathwaysHost cellsType III secretion systemBacterial effector proteinsActin-binding proteinsSignal transduction pathwaysBacterial effectorsMembrane rufflesEffector proteinsActin cytoskeletonMembrane rufflingSecretion systemSalmonella proteinsBacterial entryBacterial internalizationSalmonella entrySignaling processesActin filamentsF-actinNonphagocytic cellsProteinCell contactMyosin-V is a processive actin-based motor
Mehta A, Rock R, Rief M, Spudich J, Mooseker M, Cheney R. Myosin-V is a processive actin-based motor. Nature 1999, 400: 590-593. PMID: 10448864, DOI: 10.1038/23072.Peer-Reviewed Original ResearchThe Tail of a Yeast Class V Myosin, Myo2p, Functions as a Localization Domain
Reck-Peterson S, Novick P, Mooseker M. The Tail of a Yeast Class V Myosin, Myo2p, Functions as a Localization Domain. Molecular Biology Of The Cell 1999, 10: 1001-1017. PMID: 10198053, PMCID: PMC25227, DOI: 10.1091/mbc.10.4.1001.Peer-Reviewed Original ResearchActinsCarrier ProteinsCell FractionationCell PolarityFungal ProteinsKineticsMutagenesis, Site-DirectedMyosin Heavy ChainsMyosin Type IIMyosin Type VMyosinsPhenotypePolymerase Chain ReactionRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsSubcellular FractionsRole of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization
Zhou D, Mooseker M, Galán J. Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization. Science 1999, 283: 2092-2095. PMID: 10092234, DOI: 10.1126/science.283.5410.2092.Peer-Reviewed Original ResearchA contractile activity that closes phagosomes in macrophages
Swanson J, Johnson M, Beningo K, Post P, Mooseker M, Araki N. A contractile activity that closes phagosomes in macrophages. Journal Of Cell Science 1999, 112: 307-316. PMID: 9885284, DOI: 10.1242/jcs.112.3.307.Peer-Reviewed Original Research
1998
Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport
Evans L, Lee A, Bridgman P, Mooseker M. Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport. Journal Of Cell Science 1998, 111: 2055-2066. PMID: 9645952, DOI: 10.1242/jcs.111.14.2055.Peer-Reviewed Original ResearchConceptsMyosin VVesicle proteinsTotal vesicle proteinSynaptic vesicle proteinsInitial fractionation stepSynaptic vesicle marker proteinActin transportBrain myosin-VOrganelle transportActin filament motilityOrganelle motorFunctional analysisVesicle fractionFunction-blocking antibodiesLocalization studiesMarker proteinsImmunoelectron microscopyMotility assaysMotor domainProteinVesiclesFilament motilityVesicle integrityActinVesicle surfaceHuman myosin-IXb is a mechanochemically active motor and a GAP for rho
Post P, Bokoch G, Mooseker M. Human myosin-IXb is a mechanochemically active motor and a GAP for rho. Journal Of Cell Science 1998, 111: 941-950. PMID: 9490638, DOI: 10.1242/jcs.111.7.941.Peer-Reviewed Original ResearchActinsAdenosine TriphosphateAlternative SplicingAmino Acid SequenceAnimalsCalciumCalmodulinGTP PhosphohydrolasesGTPase-Activating ProteinsGTP-Binding ProteinsHumansLeukocytesMolecular Sequence DataMuscle ContractionMyosinsProtein BindingProteinsRas GTPase-Activating ProteinsRas ProteinsRatsRho GTP-Binding Proteins
1997
An actin-related protein in Drosophila colocalizes with heterochromatin protein 1 in pericentric heterochromatin
Frankel S, Sigel E, Craig C, Elgin S, Mooseker M, Artavanis-Tsakonas S. An actin-related protein in Drosophila colocalizes with heterochromatin protein 1 in pericentric heterochromatin. Journal Of Cell Science 1997, 110: 1999-2012. PMID: 9378752, DOI: 10.1242/jcs.110.17.1999.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAntibodiesBacterial ProteinsCarrier ProteinsCell NucleusChromatinChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneChromosomesDrosophila melanogasterEmbryo, NonmammalianEuchromatinGene Expression Regulation, DevelopmentalGenes, InsectHeterochromatinMutagenesisNuclear ProteinsRatsConceptsHeterochromatin protein 1Actin-related proteinsPolytene chromosome spreadsLocalization patternsCentric heterochromatinChromosome spreadsEuchromatic bandsProtein 1Heterochromatin-mediated geneNuclear localization patternPericentric heterochromatinHeterochromatin functionChromatin structureNuclear functionsFunctional diversitySequence similarityPolytene nucleiArp4Larval tissuesPrepupal developmentGenetic evidenceMutant formsGenetic dataHeterochromatinCell cycleActin-binding membrane proteins identified by F-actin blot overlays.
Luna E, Pestonjamasp K, Cheney R, Strassel C, Lu T, Chia C, Hitt A, Fechheimer M, Furthmayr H, Mooseker M. Actin-binding membrane proteins identified by F-actin blot overlays. Society Of General Physiologists Series 1997, 52: 3-18. PMID: 9210216.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsActinsAmino Acid SequenceAnimalsBlotting, WesternBrainBreast NeoplasmsCattleChick EmbryoDictyosteliumElectrophoresis, Polyacrylamide GelHeLa CellsHumansIodine RadioisotopesMammalsMembrane ProteinsMiceMicrofilament ProteinsNeuroblastomaNeuropeptidesNeutrophilsSodium Dodecyl SulfateTumor Cells, CulturedConceptsBlot overlayApparent molecular massMembrane proteinsF-actinF-actin binding proteinMolecular massControl cell shapePeripheral membrane proteinsSpecialized membrane domainsCell-cell adhesionPlasma membrane-enriched fractionActin-binding proteinsMammalian cell linesCell surface extensionsMembrane-enriched fractionMembrane rufflesProtein 4.1Membrane domainsMammalian cellsDictyostelium discoideumSoil amoebaPseudopod dynamicsCell shapeEfficient chemotaxisMembrane bilayer