2022
Distinct subcellular localisation of intramyocellular lipids and reduced PKCε/PKCθ activity preserve muscle insulin sensitivity in exercise-trained mice
Gaspar R, Lyu K, Hubbard B, Leitner B, Luukkonen P, Hirabara S, Sakuma I, Nasiri A, Zhang D, Kahn M, Cline G, Pauli J, Perry R, Petersen K, Shulman G. Distinct subcellular localisation of intramyocellular lipids and reduced PKCε/PKCθ activity preserve muscle insulin sensitivity in exercise-trained mice. Diabetologia 2022, 66: 567-578. PMID: 36456864, PMCID: PMC11194860, DOI: 10.1007/s00125-022-05838-8.Peer-Reviewed Original ResearchConceptsProtein kinase CsSubcellular compartmentsDistinct subcellular localisationMuscle insulin sensitivityMultiple subcellular compartmentsInsulin receptor kinaseNovel protein kinase CsActivation of PKCεSubcellular localisationPKCθ translocationReceptor kinasePlasma membraneSubcellular distributionTriacylglycerol contentCrucial pathwaysIntramuscular triacylglycerol contentRC miceDiacylglycerolConclusions/interpretationThese resultsPKCεPM compartmentPhosphorylationMuscle triacylglycerol contentSkeletal muscleRecent findings
2012
Fatty acid amide hydrolase ablation promotes ectopic lipid storage and insulin resistance due to centrally mediated hypothyroidism
Brown WH, Gillum MP, Lee HY, Camporez JP, Zhang XM, Jeong JK, Alves TC, Erion DM, Guigni BA, Kahn M, Samuel VT, Cravatt BF, Diano S, Shulman GI. Fatty acid amide hydrolase ablation promotes ectopic lipid storage and insulin resistance due to centrally mediated hypothyroidism. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 14966-14971. PMID: 22912404, PMCID: PMC3443187, DOI: 10.1073/pnas.1212887109.Peer-Reviewed Original ResearchMeSH KeywordsAmidesAmidohydrolasesAnalysis of VarianceAnimalsArachidonic AcidsChromatography, LiquidEndocannabinoidsEnergy MetabolismEthanolaminesHypothyroidismImmunoblottingInsulin ResistanceMiceMice, KnockoutPalmitic AcidsPolymerase Chain ReactionPolyunsaturated AlkamidesPPAR gammaTandem Mass SpectrometryThyrotropinThyrotropin-Releasing HormoneThyroxineTriiodothyronineConceptsEctopic lipid storageHepatic insulin resistanceInsulin resistanceEnergy expenditureDiet-induced hepatic insulin resistanceHypothalamic thyrotropin-releasing hormoneFatty acid amide hydrolase knockout miceThyroid-stimulating hormoneThyrotropin-releasing hormoneLipid storageDeiodinase 2 expressionReduced mRNA expressionProtein kinase Cε activationHepatic diacylglycerol contentPituitary thyroid-stimulating hormoneExcess energy storageFAAH deletionKnockout miceReceptor γThyroid axisThyroxine concentrationsMRNA expressionMiceHypothyroidismFAAH