2021
Sex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine Exposure
Lee AM, Mansuri MS, Wilson RS, Lam TT, Nairn AC, Picciotto MR. Sex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine Exposure. Frontiers In Molecular Neuroscience 2021, 14: 657064. PMID: 34335180, PMCID: PMC8317211, DOI: 10.3389/fnmol.2021.657064.Peer-Reviewed Original ResearchVentral tegmental areaC3H/HeJ miceGlial fibrillary acidic proteinChronic nicotine administrationNicotine administrationProtein abundanceProteomeIsobaric labelingNicotine exposureFemale miceTegmental areaHeJ miceNucleus accumbensNicotine addictionProteinSex differencesSample fractionationPathwayFibrillary acidic proteinTandem mass spectrometryNetwork analysisMouse strainsChronic nicotineMesolimbic systemNicotine reward
2019
Nicotinic Acetylcholine Receptor Signaling in the Hypothalamus: Mechanisms Related to Nicotine’s Effects on Food Intake
Calarco CA, Picciotto MR. Nicotinic Acetylcholine Receptor Signaling in the Hypothalamus: Mechanisms Related to Nicotine’s Effects on Food Intake. Nicotine & Tobacco Research 2019, 22: 152-163. PMID: 30690485, PMCID: PMC7297099, DOI: 10.1093/ntr/ntz010.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsFood intakeNicotinic acetylcholine receptorsWeight gainBody weightPreclinical workAcetylcholine receptorsPost-cessation weight gainOrexigenic agouti-related peptideAnorexigenic proopiomelanocortin (POMC) neuronsNicotine replacement therapyAgouti-related peptideNicotinic agonist cytisineAbility of nicotineLower body weightProopiomelanocortin neuronsNicotine administrationHypothalamic circuitsReplacement therapyArcuate nucleusNicotine effectsNicotine actsAgonist cytisineAverage weight gainTobacco useRodent models
2018
Evaluation of the Phosphoproteome of Mouse Alpha 4/Beta 2-Containing Nicotinic Acetylcholine Receptors In Vitro and In Vivo
Miller MB, Wilson RS, Lam TT, Nairn AC, Picciotto MR. Evaluation of the Phosphoproteome of Mouse Alpha 4/Beta 2-Containing Nicotinic Acetylcholine Receptors In Vitro and In Vivo. Proteomes 2018, 6: 42. PMID: 30326594, PMCID: PMC6313896, DOI: 10.3390/proteomes6040042.Peer-Reviewed Original ResearchProtein kinase APhosphorylation sitesPKA sitesHEK cellsNicotinic acetylcholine receptorsCalmodulin-dependent protein kinase IICalcium/calmodulin-dependent protein kinase IIProtein kinase IIAcetylcholine receptorsKinase AKinase IIMouse brainSubunit phosphorylationNovel siteSubunitsAcute nicotine administrationΒ2 subunitMammalian brainCaMKIIPhosphorylationReceptor functionΑ4 subunitHuman brain tissueBeta 2Nicotine administration
2017
Access to nicotine in drinking water reduces weight gain without changing caloric intake on high fat diet in male C57BL/6J mice
Calarco CA, Lee S, Picciotto MR. Access to nicotine in drinking water reduces weight gain without changing caloric intake on high fat diet in male C57BL/6J mice. Neuropharmacology 2017, 123: 210-220. PMID: 28623168, PMCID: PMC5544033, DOI: 10.1016/j.neuropharm.2017.06.012.Peer-Reviewed Original ResearchConceptsHigh-fat dietCaloric intakeWeight gainFat dietFemale miceMale miceBody weightAdipose tissueEffects of HFDOverall caloric intakeAmphetamine-regulated transcriptNicotinic acetylcholine receptor subunitsLower body weightMelanin-concentrating hormoneSex-dependent mannerRegulation of agoutiChow-fed animalsAcetylcholine receptor subunitsNicotine administrationChronic consumptionArcuate nucleusNicotine dosesNicotine intakeFood intakeTobacco use
2015
Modulation of aggressive behavior in mice by nicotinic receptor subtypes
Lewis AS, Mineur YS, Smith PH, Cahuzac EL, Picciotto MR. Modulation of aggressive behavior in mice by nicotinic receptor subtypes. Biochemical Pharmacology 2015, 97: 488-497. PMID: 26212554, PMCID: PMC4600457, DOI: 10.1016/j.bcp.2015.07.019.Peer-Reviewed Original ResearchConceptsAcute nicotine administrationNicotine administrationHypolocomotor effectNicotinic acetylcholine receptor agonist nicotineAgonist GTS-21Nicotinic receptor subtypesAnti-aggressive propertiesDihydro-β-erythroidineBALB/cNeurobiology of aggressionSocial interaction timeCurrent pharmacotherapyAntagonist methyllycaconitineC57BL/6 miceWorse outcomesGTS-21Receptor subtypesPathological aggressionAgonist nicotineΑ7 nAChRsSpecific treatmentSide effectsPharmacological studiesNeuropsychiatric conditionsNicotineReduction of Aggressive Episodes After Repeated Transdermal Nicotine Administration in a Hospitalized Adolescent with Autism Spectrum Disorder
Van Schalkwyk GI, Lewis AS, Qayyum Z, Koslosky K, Picciotto MR, Volkmar FR. Reduction of Aggressive Episodes After Repeated Transdermal Nicotine Administration in a Hospitalized Adolescent with Autism Spectrum Disorder. Journal Of Autism And Developmental Disorders 2015, 45: 3061-3066. PMID: 25982311, PMCID: PMC4755349, DOI: 10.1007/s10803-015-2471-0.Peer-Reviewed Original ResearchConceptsTransdermal nicotineNicotine administrationAutism spectrum disorderTransdermal nicotine administrationAdjunctive medicationsCurrent pharmacotherapyNicotine patchCholinergic dysfunctionEmergency medicationPreclinical studiesSpectrum disorderAnimal studiesAcetylcholinergic neurotransmissionNeuropsychiatric conditionsAggressive episodesMajor causeNicotineASD pathophysiologyMedicationsMorbidityFurther studiesAggression-related behaviorsAdministrationDisordersAdolescents
2010
Mice lacking the galanin gene show decreased sensitivity to nicotine conditioned place preference
Neugebauer NM, Henehan RM, Hales CA, Picciotto MR. Mice lacking the galanin gene show decreased sensitivity to nicotine conditioned place preference. Pharmacology Biochemistry And Behavior 2010, 98: 87-93. PMID: 21172385, PMCID: PMC3030658, DOI: 10.1016/j.pbb.2010.12.015.Peer-Reviewed Original ResearchConceptsGal-/- miceAcute nicotine administrationNicotine CPPPlace preferenceNicotine administrationRewarding effectsRole of galaninEffects of nicotineNucleus accumbens shellClass of drugsAmphetamine place preferenceDrugs of abuseGalanin signalingNeuropeptide galaninCPP chamberAlcohol drinkingNicotine rewardSignificant CPPExtracellular signal-related kinaseAccumbens shellGalanin peptideHigh doseSystem activationAlcohol preferenceSignal-related kinaseOral nicotine consumption does not affect maternal care or early development in mice but results in modest hyperactivity in adolescence
Heath CJ, Horst NK, Picciotto MR. Oral nicotine consumption does not affect maternal care or early development in mice but results in modest hyperactivity in adolescence. Physiology & Behavior 2010, 101: 764-769. PMID: 20826170, PMCID: PMC2975773, DOI: 10.1016/j.physbeh.2010.08.021.Peer-Reviewed Original ResearchConceptsNicotine administrationMaternal behaviorNeuropharmacological effectsSignificant between-group differencesDrinking water administrationNicotine-exposed micePostnatal weight gainBetween-group differencesOral nicotine consumptionPersistent behavioral alterationsExposure-induced changesNursing miceTransient hyperactivityDrinking waterNicotine exposureEffects of exposureC57BL/6J miceHuman smokingBehavioral alterationsNicotine consumptionPassive nursingWeight gainMiceAdministrationMaternal care
2009
Localized low‐level re‐expression of high‐affinity mesolimbic nicotinic acetylcholine receptors restores nicotine‐induced locomotion but not place conditioning
Mineur YS, Brunzell DH, Grady SR, Lindstrom JM, McIntosh JM, Marks MJ, King SL, Picciotto MR. Localized low‐level re‐expression of high‐affinity mesolimbic nicotinic acetylcholine receptors restores nicotine‐induced locomotion but not place conditioning. Genes Brain & Behavior 2009, 8: 257-266. PMID: 19077117, PMCID: PMC2672109, DOI: 10.1111/j.1601-183x.2008.00468.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsConditioning, PsychologicalCyclic AMP Response Element-Binding ProteinDopamineGamma-Aminobutyric AcidGene Expression RegulationLocomotionMiceMice, Inbred C57BLMice, TransgenicNicotineNicotinic AgonistsPhosphorylationPresynaptic TerminalsReceptors, NicotinicRewardSynaptosomesTobacco Use DisorderVentral Tegmental AreaConceptsVentral tegmental areaGamma-aminobutyric acidNicotinic acetylcholine receptorsCyclic AMP response element binding proteinTegmental areaPlace preferenceTransgenic miceAcetylcholine receptorsBeta2 knockout micePedunculopontine tegmental areaSystemic nicotine administrationHigh-affinity nAChRsLaterodorsal tegmental nucleusNicotine place preferenceNicotinic partial agonistPlace preference testingDifferent neuronal subtypesAMP response element binding proteinTotal CREB levelsResponse element-binding proteinDA neuronsCholinergic neuronsNicotine administrationSystemic nicotineSmoking cessation
2008
Role of β2-containing nicotinic acetylcholine receptors in auditory event-related potentials
Rudnick ND, Koehler C, Picciotto MR, Siegel SJ. Role of β2-containing nicotinic acetylcholine receptors in auditory event-related potentials. Psychopharmacology 2008, 202: 745-751. PMID: 18931833, DOI: 10.1007/s00213-008-1358-6.Peer-Reviewed Original ResearchConceptsAuditory event-related potentialsEffects of nicotineWild-type miceN40 amplitudeNicotine effectsSpecific nicotinic acetylcholine receptor subtypesNicotinic acetylcholine receptor subtypesΒ2-containing receptorsAcetylcholine receptor subtypesEvent-related potentialsSensory processingΒ2 knockout miceSchizophrenic individualsNicotinic acetylcholine receptorsModulates sensory processingAuditory ERP responsesNicotine administrationReceptor subtypesCA3 regionKnockout miceTherapeutic targetClick stimuliN40 componentStereotaxic implantationAcetylcholine receptors
2007
It is not “either/or”: Activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood
Picciotto MR, Addy NA, Mineur YS, Brunzell DH. It is not “either/or”: Activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood. Progress In Neurobiology 2007, 84: 329-342. PMID: 18242816, PMCID: PMC2390914, DOI: 10.1016/j.pneurobio.2007.12.005.Peer-Reviewed Original ResearchConceptsDesensitization of nAChRsNicotinic acetylcholine receptorsNicotine addictionDrug reinforcementAcetylcholine receptorsAntidepressant-like effectsActivation of nAChRsEffects of nicotineNicotine-mediated behaviorsRecent electrophysiological studiesNicotine administrationTobacco smokingAffective modulationNicotine resultsElectrophysiological studiesSmoking behaviorNicotinic agentsReceptor desensitizationDrug rewardBehavioral consequencesAffective behaviorBehavioral processesBehavioral effectsNAChRsDesensitization
2004
β2-subunit-containing nicotinic acetylcholine receptors are critical for dopamine-dependent locomotor activation following repeated nicotine administration
King SL, Caldarone BJ, Picciotto MR. β2-subunit-containing nicotinic acetylcholine receptors are critical for dopamine-dependent locomotor activation following repeated nicotine administration. Neuropharmacology 2004, 47: 132-139. PMID: 15464132, DOI: 10.1016/j.neuropharm.2004.06.024.Peer-Reviewed Original ResearchConceptsLocomotor activationNicotine administrationDopamine systemNicotinic acetylcholine receptorsAcetylcholine receptorsHigh-affinity nicotinic acetylcholine receptorsDopamine receptor antagonist pimozideBeta2 subunitRole of beta2Chronic nicotine administrationChronic nicotine exposureMesolimbic dopamine systemChronic nicotineNicotine exposureAntagonist pimozideB6 miceC57BL/6J miceOral administrationDopaminergic activationOngoing activationKnockout miceLocomotor activityMiceAdministrationNicotine
2002
Effect of nicotine and nicotinic receptors on anxiety and depression
Picciotto MR, Brunzell DH, Caldarone BJ. Effect of nicotine and nicotinic receptors on anxiety and depression. Neuroreport 2002, 13: 1097-1106. PMID: 12151749, DOI: 10.1097/00001756-200207020-00006.Peer-Reviewed Original ResearchConceptsNicotine treatmentActivation of nAChRsDorsal raphe nucleusEffects of nicotineMesolimbic dopamine systemNicotinic acetylcholine receptorsNicotine administrationMonoaminergic transmissionChronic useRaphe nucleusAnxiolytic actionLateral septumNicotinic receptorsClassical neurotransmittersNormal brainAnimal studiesDopamine systemBrain areasAcetylcholine receptorsNicotineEnsemble of studiesNAChRsAnxiety modelsAdministration studiesDepressionAcute and long‐term changes in the mesolimbic dopamine pathway after systemic or local single nicotine injections
Ferrari R, Le Novère N, Picciotto MR, Changeux JP, Zoli M. Acute and long‐term changes in the mesolimbic dopamine pathway after systemic or local single nicotine injections. European Journal Of Neuroscience 2002, 15: 1810-1818. PMID: 12081661, DOI: 10.1046/j.1460-9568.2001.02009.x.Peer-Reviewed Original ResearchMeSH KeywordsAmphetamineAnimalsDopamineDopamine Plasma Membrane Transport ProteinsDrug Administration RoutesExtracellular SpaceHabituation, PsychophysiologicMaleMembrane GlycoproteinsMembrane Transport ProteinsMicrodialysisMotor ActivityNerve Tissue ProteinsNeural PathwaysNeuronal PlasticityNicotineNucleus AccumbensPresynaptic TerminalsRatsRats, Sprague-DawleyReceptors, AMPAReceptors, N-Methyl-D-AspartateRNA, MessengerSubstance-Related DisordersTime FactorsTyrosine 3-MonooxygenaseVentral Tegmental AreaConceptsDialysate DA levelsGluR1 mRNA levelsSingle nicotine injectionNicotine administrationDA levelsMesolimbic dopamine pathwayNicotine injectionTyrosine hydroxylaseDopamine pathwayMRNA levelsBasal DA levelsExtracellular DA levelsMesolimbic DA pathwaySystemic intraperitoneal injectionTH mRNA levelsIntra-accumbens infusion
2000
Nicotinic modulation of mesoprefrontal dopamine neurons: pharmacologic and neuroanatomic characterization.
George TP, Verrico CD, Picciotto MR, Roth RH. Nicotinic modulation of mesoprefrontal dopamine neurons: pharmacologic and neuroanatomic characterization. Journal Of Pharmacology And Experimental Therapeutics 2000, 295: 58-66. PMID: 10991961.Peer-Reviewed Original ResearchConceptsMesoprefrontal DA neuronsDA neuronsLocal infusionNicotinic acetylcholine receptor stimulationMesoprefrontal dopamine neuronsMesoprefrontal dopamine systemEndogenous opioid peptidesVentral tegmental areaHigh comorbid ratesNicotine-pretreated ratsMedial prefrontal cortexDA metabolismNicotinic modulationCortical dysfunctionNicotine administrationCigarette smokingSite of actionStress-induced statesNicotine pretreatmentDA dysregulationDopamine neuronsSystemic administrationTegmental areaDA dysfunctionCognitive dysfunction