2015
DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons
Engmann O, Giralt A, Gervasi N, Marion-Poll L, Gasmi L, Filhol O, Picciotto MR, Gilligan D, Greengard P, Nairn AC, Hervé D, Girault JA. DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons. Nature Communications 2015, 6: 10099. PMID: 26639316, PMCID: PMC4675091, DOI: 10.1038/ncomms10099.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBehavior, AnimalBrainCaffeineCalmodulin-Binding ProteinsCentral Nervous System StimulantsChlorocebus aethiopsCocaineCOS CellsDendritic SpinesDopamine and cAMP-Regulated Phosphoprotein 32EnvironmentFluorescence Recovery After PhotobleachingImmunoblottingImmunohistochemistryIn Vitro TechniquesMass SpectrometryMiceMice, Inbred C57BLMutationNeostriatumNeuronsNucleus AccumbensPhosphorylationRatsRats, Sprague-DawleyRewardConceptsAdducin phosphorylationCytoskeletal proteinsActin filamentsMolecular pathwaysCellular mechanismsEnvironmental changesPhosphorylationDARPP-32Striatal neuronsAdducinMutant miceSynaptic stabilityProteinCascadeMultiple effectsEnvironmental effectsBindsDendritic spinesNeuronsModification of responsesBrief exposurePathwayInteractionFilamentsEnrichment
2007
Deficiency in Inhibitory Cortical Interneurons Associates with Hyperactivity in Fibroblast Growth Factor Receptor 1 Mutant Mice
Smith K, Fagel DM, Stevens HE, Rabenstein RL, Maragnoli ME, Ohkubo Y, Picciotto MR, Schwartz ML, Vaccarino FM. Deficiency in Inhibitory Cortical Interneurons Associates with Hyperactivity in Fibroblast Growth Factor Receptor 1 Mutant Mice. Biological Psychiatry 2007, 63: 953-962. PMID: 17988653, DOI: 10.1016/j.biopsych.2007.09.020.Peer-Reviewed Original ResearchMeSH KeywordsAmphetamineAnimalsBehavior, AnimalBiogenic MonoaminesCell CountCentral Nervous System StimulantsCerebral CortexDisease Models, AnimalDopamine AgentsExploratory BehaviorFibroblast Growth Factor 1Glutamate DecarboxylaseHyperkinesisLocomotionMaleMethylphenidateMiceMice, KnockoutMotor ActivityNerve Tissue ProteinsNeural InhibitionNeuronsSignal TransductionConceptsInhibitory cortical circuitsCortical pyramidal neuronsD2 receptor antagonistGrowth factor receptor 1Spontaneous locomotor hyperactivityFibroblast growth factor receptor 1Factor receptor 1Inhibitory neuronal subtypesLocomotor hyperactivityDopamine agonistsCerebral cortexPyramidal neuronsBasal gangliaMotor hyperactivityReceptor antagonistInhibitory interneuronsTyrosine hydroxylaseCortical circuitsPsychiatric disordersLocomotor responseNeuronal subtypesReceptor 1Mutant miceDopamine transporterSpatial learning
2006
The Prototoxin lynx1 Acts on Nicotinic Acetylcholine Receptors to Balance Neuronal Activity and Survival In Vivo
Miwa JM, Stevens TR, King SL, Caldarone BJ, Ibanez-Tallon I, Xiao C, Fitzsimonds RM, Pavlides C, Lester HA, Picciotto MR, Heintz N. The Prototoxin lynx1 Acts on Nicotinic Acetylcholine Receptors to Balance Neuronal Activity and Survival In Vivo. Neuron 2006, 51: 587-600. PMID: 16950157, DOI: 10.1016/j.neuron.2006.07.025.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAge FactorsAnimalsAssociation LearningBrainCell SurvivalExcitatory Amino Acid AgonistsMembrane GlycoproteinsMembrane PotentialsMiceMice, Mutant StrainsMutationNerve DegenerationNeuronsNeuropeptidesNicotineNicotinic AgonistsPatch-Clamp TechniquesReceptors, NicotinicConceptsNicotinic acetylcholine receptorsNull mutant miceMutant miceAcetylcholine receptorsNeuronal activityNull mutationBiological processesLynx1Calcium levelsAgonist sensitivityReceptor desensitizationSynaptic efficacyMutationsAllosteric modulatorsDesensitization kineticsWide arrayNicotineVivoSpecific testsNAChRsMiceReceptorsSurvivalHyperactivationFunction
2004
Role of neuronal nicotinic receptors in the effects of nicotine and ethanol on contextual fear conditioning
Wehner JM, Keller JJ, Keller AB, Picciotto MR, Paylor R, Booker TK, Beaudet A, Heinemann SF, Balogh SA. Role of neuronal nicotinic receptors in the effects of nicotine and ethanol on contextual fear conditioning. Neuroscience 2004, 129: 11-24. PMID: 15489024, DOI: 10.1016/j.neuroscience.2004.07.016.Peer-Reviewed Original ResearchConceptsContextual learningForms of learningEffects of nicotineFear conditioningCued fear conditioningContextual fear conditioningCognitive enhancing effectsBeta2 null mutant miceNicotine effectsBeta4-containing receptorsShock stimuliEffects of ethanolBeta2-containing nAChRsLearningNull mutant miceTrainingConditioningBrain nicotinic acetylcholine receptorsMutant miceNicotineNeuronal nicotinic receptorsHomozygous null mutant miceWild-type littermatesMemoryNicotinic acetylcholine receptors
2003
α4β2* Nicotinic Acetylcholine Receptors Modulate the Effects of Ethanol and Nicotine on the Acoustic Startle Response
Owens JC, Balogh SA, McClure‐Begley T, Butt CM, Labarca C, Lester HA, Picciotto MR, Wehner JM, Collins AC. α4β2* Nicotinic Acetylcholine Receptors Modulate the Effects of Ethanol and Nicotine on the Acoustic Startle Response. Alcohol Clinical And Experimental Research 2003, 27: 1867-1875. PMID: 14691373, DOI: 10.1097/01.alc.0000102700.72447.0f.Peer-Reviewed Original ResearchConceptsAcoustic startle responseEffects of ethanolLSxSS RIStartle responseAcoustic startleAlpha4beta2 nAChRsEthanol effectsBeta2 null mutant miceNeuronal nicotinic cholinergic receptorsEthanol-induced depressionNicotinic cholinergic receptorsPotential roleNicotinic acetylcholine receptorsNull mutant miceAlpha4 nAChRsCholinergic receptorsTobacco abuseNicotine effectsT polymorphismAcetylcholine receptorsLSXSS recombinantMutant miceBehavioral effectsNicotineStartle
2002
Characterization of [125I]epibatidine binding and nicotinic agonist‐mediated 86Rb+ efflux in interpeduncular nucleus and inferior colliculus of β2 null mutant mice
Marks MJ, Whiteaker P, Grady SR, Picciotto MR, McIntosh JM, Collins AC. Characterization of [125I]epibatidine binding and nicotinic agonist‐mediated 86Rb+ efflux in interpeduncular nucleus and inferior colliculus of β2 null mutant mice. Journal Of Neurochemistry 2002, 81: 1102-1115. PMID: 12065623, DOI: 10.1046/j.1471-4159.2002.00910.x.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAlkaloidsAnimalsAzocinesBinding, CompetitiveBridged Bicyclo Compounds, HeterocyclicDose-Response Relationship, DrugInferior ColliculiIodine RadioisotopesMesencephalonMiceMice, Mutant StrainsNicotinic AgonistsNicotinic AntagonistsPyridinesQuinolizinesReceptors, NicotinicRubidium RadioisotopesTritiumConceptsInterpeduncular nucleusInferior colliculusBrain regionsAccessory olfactory nucleusNull mutant miceOlfactory nucleusNicotinic antagonistsD-tubocurarineMedial habenulaSelective antagonistNicotinic agonistsSuperior colliculusMouse brainAgonistsColliculusMutant micePotent agonistSimilar potencyAntagonistNicotinic activityEfflux
1999
Assessment of nicotinic acetylcholine receptor subunit contributions to nicotine self-administration in mutant mice
Epping-Jordan M, Picciotto M, Changeux J, Pich EM. Assessment of nicotinic acetylcholine receptor subunit contributions to nicotine self-administration in mutant mice. Psychopharmacology 1999, 147: 25-26. PMID: 10591862, DOI: 10.1007/s002130051135.Peer-Reviewed Original ResearchTwo pharmacologically distinct components of nicotinic receptor-mediated rubidium efflux in mouse brain require the beta2 subunit.
Marks MJ, Whiteaker P, Calcaterra J, Stitzel JA, Bullock AE, Grady SR, Picciotto MR, Changeux JP, Collins AC. Two pharmacologically distinct components of nicotinic receptor-mediated rubidium efflux in mouse brain require the beta2 subunit. Journal Of Pharmacology And Experimental Therapeutics 1999, 289: 1090-103. PMID: 10215692.Peer-Reviewed Original ResearchConceptsBeta2 subunitBeta2 null mutant miceConcentration-effect curvesMouse brain synaptosomesAlpha4beta2 receptorsBrain synaptosomesNicotinic agonistsMouse brainRubidium effluxMutant miceLine radioactivity detectionDHbetaEAgonistsEffluxBrainStimulationRadioactivity detectionPotencyHexamethoniumErythroidineResponseAcetylcholineMethyllycaconitineAntagonistBungarotoxinModulation of morphine analgesia in αCGRP mutant mice
Salmon A, Damaj I, Sekine S, Picciotto M, Marubio L, Changeux J. Modulation of morphine analgesia in αCGRP mutant mice. Neuroreport 1999, 10: 849-854. PMID: 10208559, DOI: 10.1097/00001756-199903170-00033.Peer-Reviewed Original ResearchConceptsHot plate testMorphine analgesiaMutant miceTail-flick testPlate testWild-type miceMotor end platesPain pathwaysCGRP immunoreactivityMice lackFlick testMuscle synapsesAntinociceptive behaviorSpinal cordTail flickSpinal gangliaMiceAnalgesiaTargeted disruptionExon 5End platesMorphineGangliaCordImmunoreactivityIncreased neurodegeneration during ageing in mice lacking high‐affinity nicotine receptors
Zoli M, Picciotto M, Ferrari R, Cocchi D, Changeux J. Increased neurodegeneration during ageing in mice lacking high‐affinity nicotine receptors. The EMBO Journal 1999, 18: 1235-1244. PMID: 10064590, PMCID: PMC1171214, DOI: 10.1093/emboj/18.5.1235.Peer-Reviewed Original ResearchConceptsHigh-affinity nicotine receptorsSpatial learningHippocampal pyramidal neuronsRegion-specific alterationsSerum corticosterone levelsPossible animal modelNicotinic acetylcholine receptorsPyramidal neuronsNeuronal survivalNicotine receptorsCholinergic systemEndocrine parametersControl animalsIncreased neurodegenerationAnimal modelsCorticosterone levelsAcetylcholine receptorsAlzheimer's diseaseDegenerative processCortical regionsMorris mazeCognitive deficitsMutant miceMiceBeta2 subunit
1998
Pharmacological characterization of nicotinic receptor-stimulated GABA release from mouse brain synaptosomes.
Lu Y, Grady S, Marks M, Picciotto M, Changeux J, Collins A. Pharmacological characterization of nicotinic receptor-stimulated GABA release from mouse brain synaptosomes. Journal Of Pharmacology And Experimental Therapeutics 1998, 287: 648-57. PMID: 9808692.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidGABA releaseMouse brain synaptosomesBrain synaptosomesNicotinic agonistsNicotine bindingBeta2 null mutant miceNull mutant miceRecent electrophysiological studiesRodent brain tissueConcentration-dependent mannerAgonist inhibitionAlpha-BungarotoxinNeurochemical approachesPharmacological characterizationNM tetrodotoxinElectrophysiological studiesSame agonistBrain regionsBrain tissueMutant miceAgonistsEmax valuesBeta2 subunitMaximal releaseUsing Knockout and Transgenic Mice to Study Neurophysiology and Behavior
PICCIOTTO M, WICKMAN K. Using Knockout and Transgenic Mice to Study Neurophysiology and Behavior. Physiological Reviews 1998, 78: 1131-1163. PMID: 9790572, DOI: 10.1152/physrev.1998.78.4.1131.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsAcetylcholine receptors containing the β2 subunit are involved in the reinforcing properties of nicotine
Picciotto M, Zoli M, Rimondini R, Léna C, Marubio L, Pich E, Fuxe K, Changeux J. Acetylcholine receptors containing the β2 subunit are involved in the reinforcing properties of nicotine. Nature 1998, 391: 173-177. PMID: 9428762, DOI: 10.1038/34413.Peer-Reviewed Original ResearchMeSH Keywords3,4-Dihydroxyphenylacetic AcidAcetylcholineAnimalsBinding SitesCarrier ProteinsCocaineConditioning, OperantDopamineDopamine Plasma Membrane Transport ProteinsHomovanillic AcidIn Vitro TechniquesMaleMembrane GlycoproteinsMembrane Transport ProteinsMiceMice, Inbred C57BLMice, Inbred DBAMice, KnockoutMicrodialysisMotor ActivityNerve Tissue ProteinsNicotineNucleus AccumbensPatch-Clamp TechniquesReceptors, NicotinicSecond Messenger SystemsSubstantia NigraVentral Tegmental AreaConceptsProperties of nicotineAcetylcholine receptorsVentral striatumΒ2 subunitNeuronal nicotinic acetylcholine receptorsMesencephalic dopaminergic neuronsEffects of nicotineWild-type micePatch-clamp recordingsMesolimbic dopamine systemNicotinic acetylcholine receptorsDrugs of abuseDopaminergic neuronsMesolimbic systemDopamine releaseDopamine systemMutant miceMiceNicotineNeurotransmitter dopamineStriatumReceptorsNeuronsReleaseBrain
1995
Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain
Picciotto M, Zoli M, Léna C, Bessis A, Lallemand Y, LeNovère N, Vincent P, Pich E, Brûlet P, Changeux J. Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain. Nature 1995, 374: 65-67. PMID: 7870173, DOI: 10.1038/374065a0.Peer-Reviewed Original ResearchConceptsHigh-affinity nicotine receptorsNeuronal nicotinic acetylcholine receptorsBrains of miceΒ2-/- miceNicotinic acetylcholine receptorsThalamic neuronsNicotine applicationFunctional nAChRsNicotine receptorsBrain slicesNicotinic subunitsAbnormal avoidanceAcetylcholine receptorsAspects of behaviorHigh-affinity binding sitesMutant miceElectrophysiological recordingsPassive avoidanceAssociative memoryMiceNicotineNeuronal nicotinic subunitsNon-mutant siblingsBrainReceptors