2016
Sensitive Detection and Simultaneous Discrimination of Influenza A and B Viruses in Nasopharyngeal Swabs in a Single Assay Using Next-Generation Sequencing-Based Diagnostics
Zhao J, Liu J, Vemula SV, Lin C, Tan J, Ragupathy V, Wang X, Mbondji-wonje C, Ye Z, Landry ML, Hewlett I. Sensitive Detection and Simultaneous Discrimination of Influenza A and B Viruses in Nasopharyngeal Swabs in a Single Assay Using Next-Generation Sequencing-Based Diagnostics. PLOS ONE 2016, 11: e0163175. PMID: 27658193, PMCID: PMC5033603, DOI: 10.1371/journal.pone.0163175.Peer-Reviewed Original ResearchB virusInfluenza AInfluenza types/subtypesInfluenza virusPandemic influenza virusInfluenza B virusTypes/subtypesInfluenza viral genomeE627K mutationAntiviral treatmentNasopharyngeal swabsInfluenza strainsLaboratory confirmationPandemic potentialS31N mutationPatient specimensClinical settingNGS assaysDrug resistance markersVirusFurther studiesCurrent diagnostic platformsPublic healthK mutationInfluenza
2015
Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses - Volume 21, Number 3—March 2015 - Emerging Infectious Diseases journal - CDC
Zhao J, Ragupathy V, Liu J, Wang X, Vemula SV, Mubarak H, Ye Z, Landry ML, Hewlett I. Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses - Volume 21, Number 3—March 2015 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2015, 21: 400-408. PMID: 25694248, PMCID: PMC4344273, DOI: 10.3201/eid2103.141169.Peer-Reviewed Original ResearchConceptsMultiplex next-generation sequencingNext-generation sequencingInfluenza virusInfectious Diseases journal - CDCPotential influenza pandemicFuture epidemiologic studiesPublic health benefitsNew diagnostic algorithmInfluenza infectionB virusInfluenza AInfluenza pandemicEpidemiologic studiesMultiple influenzaLaboratory confirmationDiagnostic algorithmHealth benefitsNGS assaysVirusInfluenzaSubtypesMatrix genesGene segmentsDiagnostic platformConfirmation
2009
Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus
Landry ML, Ferguson D. Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus. Journal Of Clinical Microbiology 2009, 48: 957-959. PMID: 20042632, PMCID: PMC2832425, DOI: 10.1128/jcm.01678-09.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsChildChild, PreschoolClinical Laboratory TechniquesFluorescent Antibody Technique, DirectHumansInfantInfant, NewbornInfluenza A Virus, H1N1 SubtypeInfluenza, HumanNasopharynxReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityVirologyYoung AdultConceptsNovel swine-origin influenza virusesSwine-origin influenza virusImmunofluorescence antibody testingReal-time reverse transcriptase PCRCycle threshold valuesPCR-positive resultsPediatric patientsCDC assayAntibody testingNasopharyngeal swabsReverse transcriptase-PCRSwine influenzaInfluenza virusTranscriptase-PCRPositive samplesPatientsPositive resultsInfluenzaSwabsCytospins
2006
Coronavirus HKU1 Infection in the United States - Volume 12, Number 5—May 2006 - Emerging Infectious Diseases journal - CDC
Esper F, Weibel C, Ferguson D, Landry ML, Kahn JS. Coronavirus HKU1 Infection in the United States - Volume 12, Number 5—May 2006 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2006, 12: 775-779. PMID: 16704837, PMCID: PMC3374449, DOI: 10.3201/eid1205.051316.Peer-Reviewed Original ResearchConceptsHCoV-HKU1Respiratory specimensLower respiratory tract infectionsLower respiratory tract diseaseHCoV-HKU1 infectionRespiratory tract infectionsRespiratory syncytial virusRespiratory tract diseaseReverse transcription-polymerase chain reactionNew human coronavirusYears of ageTranscription-polymerase chain reactionTract infectionsSyncytial virusPositive childrenParainfluenza virusTract diseaseRespiratory tractHuman coronavirusesPositive specimensInfluenza virusInfectionChain reactionChildrenVirus
2003
Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence
Landry ML, Ferguson D. Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence. Journal Of Clinical Microbiology 2003, 41: 3407-3409. PMID: 12843105, PMCID: PMC165325, DOI: 10.1128/jcm.41.7.3407-3409.2003.Peer-Reviewed Original ResearchConceptsVirus infectionEnzyme immunoassayDirectigen FluParainfluenza virus infectionPositive EIA resultsRespiratory syncytial virusAntigen-positive cellsNasopharyngeal swabsSyncytial virusEmergency departmentInfluenza diagnosisDirect immunofluorescenceInfluenza virusVirology laboratoryEIA resultsNight shiftsInfectionInfluenzaImmunofluorescenceFluAdultsVirusCorrelation of resultsImmunoassayCells