2021
Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model
Habertheuer A, Ram C, Schmierer M, Chatterjee S, Hu R, Freas A, Zielinski P, Rogers W, Silvestro EM, McGrane M, Moore JS, Korutla L, Siddiqui S, Xin Y, Rizi R, Tao J, Kreisel D, Naji A, Ochiya T, Vallabhajosyula P. Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model. Transplantation 2021, 106: 754-766. PMID: 33993180, DOI: 10.1097/tp.0000000000003820.Peer-Reviewed Original ResearchConceptsAcute rejectionDonor exosomesExosome profileSyngeneic controlsRat orthotopic lung transplant modelChronic lung allograft dysfunctionOrthotopic lung transplantation modelLung transplant modelPosttransplant time pointsAcute rejection episodesLung allograft dysfunctionLung transplant patientsLung transplant rejectionLung transplantation modelEarly therapeutic interventionMajor risk factorTime-sensitive diagnosisDevelopment of biomarkersAllograft dysfunctionRejection episodesLewis recipientsTransplant patientsPulmonary allograftsRecipient bloodAllogeneic grafts
2020
Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia
Levine L, Habertheuer A, Ram C, Korutla L, Schwartz N, Hu RW, Reddy S, Freas A, Zielinski PD, Harmon J, Molugu SK, Parry S, Vallabhajosyula P. Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia. Scientific Reports 2020, 10: 6398. PMID: 32286341, PMCID: PMC7156695, DOI: 10.1038/s41598-020-62193-7.Peer-Reviewed Original ResearchConceptsSyncytin-1Preeclampsia groupMaternal circulationExtracellular vesiclesCommon placental pathologyHigh expressionDiagnosis of preeclampsiaConditions of preeclampsiaBone alkaline phosphatasePotential physiologic roleTissue-specific biomarkersExtracellular microvesiclesPlasma extracellular vesiclesAlkaline phosphatasePreeclampsia subjectsNormal pregnancyPlacental pathologyCell extracellular vesiclesImproved biomarkersMaternal bloodPreeclampsiaPlacental syncytiotrophoblastMaternal plasmaPlacental alkaline phosphatasePhysiologic role
2019
Circulating exosomes derived from transplanted progenitor cells aid the functional recovery of ischemic myocardium
Saha P, Sharma S, Korutla L, Datla SR, Shoja-Taheri F, Mishra R, Bigham GE, Sarkar M, Morales D, Bittle G, Gunasekaran M, Ambastha C, Arfat MY, Li D, Habertheuer A, Hu R, Platt MO, Yang P, Davis ME, Vallabhajosyula P, Kaushal S. Circulating exosomes derived from transplanted progenitor cells aid the functional recovery of ischemic myocardium. Science Translational Medicine 2019, 11 PMID: 31118291, PMCID: PMC6857931, DOI: 10.1126/scitranslmed.aau1168.Peer-Reviewed Original ResearchConceptsCardiac progenitor cellsMessenger RNACondition-specific mannerComputational pathway analysisProgenitor cellsCellular messenger RNAsCardiosphere-derived cellsProgenitor cell typesStem cell fieldCell-derived exosomesPathway analysisMicroRNA (miRNA) cargoCell typesExosomesTransplanted progenitor cellsCPC exosomesConditional activityPlasma exosomesC-kitCellsCell fieldTransplanted cardiac progenitor cellsRNAMyocardial infarction modelPathwayNoninvasive diagnosis of recurrent autoimmune type 1 diabetes after islet cell transplantation
Korutla L, Rickels MR, Hu RW, Freas A, Reddy S, Habertheuer A, Harmon J, Korutla V, Ram C, Naji A, Vallabhajosyula P. Noninvasive diagnosis of recurrent autoimmune type 1 diabetes after islet cell transplantation. American Journal Of Transplantation 2019, 19: 1852-1858. PMID: 30801971, PMCID: PMC7043773, DOI: 10.1111/ajt.15322.Peer-Reviewed Original ResearchConceptsIslet cell transplantationAutoimmune type 1 diabetesType 1 diabetesCell transplantationT1D autoimmunityNoninvasive diagnosisPancreatic β-cell injuryGlutamic acid decarboxylase 65Time-matched analysisΒ-cell injuryTransplanted β-cellsHyperglycemia onsetIslet injuryRecurrent autoimmunityHypoglycemic unawarenessTime-specific increaseIslet autoantigensCurative therapyT1D patientsImmunologic rejectionExogenous insulinInjury typeControl subjectsIslet transplantationDonor islets
2018
Donor tissue-specific exosome profiling enables noninvasive monitoring of acute rejection in mouse allogeneic heart transplantation
Habertheuer A, Korutla L, Rostami S, Reddy S, Lal P, Naji A, Vallabhajosyula P. Donor tissue-specific exosome profiling enables noninvasive monitoring of acute rejection in mouse allogeneic heart transplantation. Journal Of Thoracic And Cardiovascular Surgery 2018, 155: 2479-2489. PMID: 29499866, DOI: 10.1016/j.jtcvs.2017.12.125.Peer-Reviewed Original ResearchConceptsEarly acute rejectionDonor heartsAcute rejectionHeart transplantationStudy armsImmunologic rejectionMaintenance armHeterotopic heart transplantation modelAllogeneic heart transplantationTime pointsHeart transplantation modelHeterotopic heart transplantationAbsence of rejectionNovel biomarker platformDevelopment of biomarkersNoninvasive monitoringTransplant heartCardiac allograftsImmunocompetent recipientsAllograft monitoringImmunodeficient recipientsRecipient circulationTransplantation modelDay 1Total plasma
2017
Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue
Vallabhajosyula P, Korutla L, Habertheuer A, Yu M, Rostami S, Yuan CX, Reddy S, Liu C, Korutla V, Koeberlein B, Trofe-Clark J, Rickels MR, Naji A. Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue. Journal Of Clinical Investigation 2017, 127: 1375-1391. PMID: 28319051, PMCID: PMC5373894, DOI: 10.1172/jci87993.Peer-Reviewed Original ResearchConceptsRenal transplantationImmunologic rejectionTransplanted tissueClinical settingBiomarker platformAppearance of hyperglycemiaIslet transplant modelIslet β-cellsDonor exosomesTransplant isletsHLA antibodiesRecipient bloodTransplant modelRecipient circulationXenogeneic modelRecipient plasmaRenal epithelial cellsTransplant tissueHuman isletsΒ-cellsBiomarker potentialTransplantationNoninvasive windowEpithelial cellsExosomal microRNAs