2022
Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation
Habertheuer A, Chatterjee S, Sada Japp A, Ram C, Korutla L, Ochiya T, Li W, Terada Y, Takahashi T, Nava RG, Puri V, Kreisel D, Vallabhajosyula P. Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation. American Journal Of Transplantation 2022, 22: 1909-1918. PMID: 35285127, DOI: 10.1111/ajt.17023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsExtracellular VesiclesGraft RejectionHumansLung TransplantationLymph NodesRatsTissue DonorsConceptsMediastinal lymph nodesLymph nodesLung transplantationDonor antigensPleural spaceBronchial anastomosisGraft-draining lymph nodesLocoregional lymph nodesLung transplant recipientsPeripheral lymph nodesTransplant recipientsAllorecognition pathwaysPulmonary transplantationMHC-IIPleural fluidRat modelT cellsLymphatic drainageTransplantationDonor extracellular vesiclesPleural lymphaticsCell traffickingRapid rejectionAlternative pathwayAntigen
2021
Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model
Habertheuer A, Ram C, Schmierer M, Chatterjee S, Hu R, Freas A, Zielinski P, Rogers W, Silvestro EM, McGrane M, Moore JS, Korutla L, Siddiqui S, Xin Y, Rizi R, Tao J, Kreisel D, Naji A, Ochiya T, Vallabhajosyula P. Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model. Transplantation 2021, 106: 754-766. PMID: 33993180, DOI: 10.1097/tp.0000000000003820.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsExosomesGraft RejectionHumansLungLung TransplantationRatsRats, Inbred LewRats, WistarRodentiaConceptsAcute rejectionDonor exosomesExosome profileSyngeneic controlsRat orthotopic lung transplant modelChronic lung allograft dysfunctionOrthotopic lung transplantation modelLung transplant modelPosttransplant time pointsAcute rejection episodesLung allograft dysfunctionLung transplant patientsLung transplant rejectionLung transplantation modelEarly therapeutic interventionMajor risk factorTime-sensitive diagnosisDevelopment of biomarkersAllograft dysfunctionRejection episodesLewis recipientsTransplant patientsPulmonary allograftsRecipient bloodAllogeneic grafts
2017
Ex Vivo Lung Perfusion Model to Study Pulmonary Tissue Extracellular Microvesicle Profiles
Vallabhajosyula P, Korutla L, Habertheuer A, Reddy S, Schaufler C, Lasky J, Diamond J, Cantu E. Ex Vivo Lung Perfusion Model to Study Pulmonary Tissue Extracellular Microvesicle Profiles. The Annals Of Thoracic Surgery 2017, 103: 1758-1766. PMID: 28242077, DOI: 10.1016/j.athoracsur.2016.11.074.Peer-Reviewed Original ResearchMeSH KeywordsExtracellular VesiclesHumansLungLung TransplantationMass SpectrometryNanoparticlesPerfusionConceptsEVLP systemWestern blot analysisTransplant groupExtracellular microvesiclesVivo lung perfusion modelTime pointsMarginal donor lungsLung perfusion modelFollowing time pointsTranscription-polymerase chain reactionBlot analysisEV size distributionMarginal lungsNontransplant groupDonor lungsLung recoveryLung perfusionSteen solutionPolymerase chain reactionPulmonary parenchymaEV protein cargoPathologic processesPerfusion modelTissue-specific markersPerfusate exchange