2023
Circulating T cell specific extracellular vesicle profiles in cardiac allograft acute cellular rejection
Korutla L, Hoffman J, Rostami S, Hu R, Korutla V, Markmann C, Mullan C, Sotolongo A, Habertheuer A, Romano C, Acker M, Sen S, Agarwal D, Jayaraman A, Li B, Davis M, Naji A, Vallabhajosyula P. Circulating T cell specific extracellular vesicle profiles in cardiac allograft acute cellular rejection. American Journal Of Transplantation 2023, 24: 419-435. PMID: 38295008, DOI: 10.1016/j.ajt.2023.10.021.Peer-Reviewed Original ResearchAcute cellular rejectionAllogeneic transplant recipientsTransplant recipientsCellular rejectionHeart transplantationAlloreactive T cell activationEarly acute cellular rejectionT-cell receptor sequencingSyngeneic transplant recipientsCell receptor sequencingClinical heart transplantationT cell clonesNovel candidate biomarkersT cell activationQuantitative RT-PCRRecipient circulationOrgan transplantationT cellsVesicle profilesNoninvasive biomarkersCandidate biomarkersCell activationRegulatory markersDonor cardiomyocytesTransplantation
2022
Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation
Habertheuer A, Chatterjee S, Sada Japp A, Ram C, Korutla L, Ochiya T, Li W, Terada Y, Takahashi T, Nava RG, Puri V, Kreisel D, Vallabhajosyula P. Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation. American Journal Of Transplantation 2022, 22: 1909-1918. PMID: 35285127, DOI: 10.1111/ajt.17023.Peer-Reviewed Original ResearchConceptsMediastinal lymph nodesLymph nodesLung transplantationDonor antigensPleural spaceBronchial anastomosisGraft-draining lymph nodesLocoregional lymph nodesLung transplant recipientsPeripheral lymph nodesTransplant recipientsAllorecognition pathwaysPulmonary transplantationMHC-IIPleural fluidRat modelT cellsLymphatic drainageTransplantationDonor extracellular vesiclesPleural lymphaticsCell traffickingRapid rejectionAlternative pathwayAntigen
2020
Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes
Yu M, Agarwal D, Korutla L, May CL, Wang W, Griffith NN, Hering BJ, Kaestner KH, Velazquez OC, Markmann JF, Vallabhajosyula P, Liu C, Naji A. Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes. Nature Metabolism 2020, 2: 1013-1020. PMID: 32895576, PMCID: PMC7572844, DOI: 10.1038/s42255-020-0269-7.Peer-Reviewed Original ResearchConceptsIslet transplantationSubcutaneous spacePancreatic isletsNon-human primate modelSyngeneic islet transplantationType 1 diabetesTreatment of typeLack of neovascularizationPancreatic islet transplantationNew therapeutic paradigmHuman pancreatic isletsSustained normoglycaemiaGraft survivalHypoxic cell deathPrimate modelPreclinical modelsAnimal modelsTransplantationSubcutaneous transplantationTherapeutic paradigmDiabetesEuglycaemiaCell deathIsletsTransplantation methodologies
2017
Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue
Vallabhajosyula P, Korutla L, Habertheuer A, Yu M, Rostami S, Yuan CX, Reddy S, Liu C, Korutla V, Koeberlein B, Trofe-Clark J, Rickels MR, Naji A. Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue. Journal Of Clinical Investigation 2017, 127: 1375-1391. PMID: 28319051, PMCID: PMC5373894, DOI: 10.1172/jci87993.Peer-Reviewed Original ResearchConceptsRenal transplantationImmunologic rejectionTransplanted tissueClinical settingBiomarker platformAppearance of hyperglycemiaIslet transplant modelIslet β-cellsDonor exosomesTransplant isletsHLA antibodiesRecipient bloodTransplant modelRecipient circulationXenogeneic modelRecipient plasmaRenal epithelial cellsTransplant tissueHuman isletsΒ-cellsBiomarker potentialTransplantationNoninvasive windowEpithelial cellsExosomal microRNAs