2012
Nucleus Accumbens 1, a Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad protein binds to TAR DNA-binding protein 43 and has a potential role in Amyotrophic Lateral Sclerosis
Scofield M, Korutla L, Jackson T, Kalivas P, Mackler S. Nucleus Accumbens 1, a Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad protein binds to TAR DNA-binding protein 43 and has a potential role in Amyotrophic Lateral Sclerosis. Neuroscience 2012, 227: 44-54. PMID: 23022214, PMCID: PMC3505276, DOI: 10.1016/j.neuroscience.2012.09.043.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAspartic AcidCell DeathCholine O-AcetyltransferaseCytoplasmDNA-Binding ProteinsEmbryo, MammalianGene Expression RegulationGlial Fibrillary Acidic ProteinGlutamic AcidImmunoprecipitationNeoplasm ProteinsNeuronsPhosphopyruvate HydrataseProtein BindingRatsRepressor ProteinsSpinal CordTransfectionUbiquitinationConceptsNucleus accumbens-1Amyotrophic lateral sclerosisPOZ/BTB domainUbiquitin-proteasome systemTDP-43Protein 43Lateral sclerosisBTB domainPrimary spinal cord culturesDevelopment of ALSTAR DNA-binding protein 43POZ/BTB proteinDNA-binding protein 43Extracellular glutamate levelsTDP-43 expressionFull-length TDP-43Spinal cord culturesGST pulldown assaysPost-translational modificationsUbiquitinated protein aggregatesCholinergic neuronsGlutamate toxicityCord culturesGlutamate levelsSpinal cord
2008
NAC1, a POZ/BTB protein that functions as a corepressor
Korutla L, Wang P, Jackson T, Mackler S. NAC1, a POZ/BTB protein that functions as a corepressor. Neurochemistry International 2008, 54: 245-252. PMID: 19121354, DOI: 10.1016/j.neuint.2008.12.008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCentral Nervous SystemGenes, ReporterHumansKruppel-Like Transcription FactorsMiceNeoplasm ProteinsNerve Tissue ProteinsPromyelocytic Leukemia Zinc Finger ProteinProtein IsoformsProtein Structure, TertiaryProto-Oncogene ProteinsRecombinant Fusion ProteinsRepressor ProteinsTranscription, GeneticConceptsPOZ/BTB proteinBTB proteinsPOZ/BTB domainFusion proteinVP16 activation domainGST pulldown assaysGAL4 fusion proteinsTranscriptional repressor proteinZinc finger proteinProtein-protein interactionsB fusion proteinFinger proteinActivation domainTransient assaysNAC1 functionPulldown assaysRepressor proteinBTB domainNon-neuronal cellsTranscriptional inhibitionCorepressorNeuronal cellsProteinMature CNSSelective interaction
2007
NAC1, a cocaine‐regulated POZ/BTB protein interacts with CoREST
Korutla L, Degnan R, Wang P, Mackler S. NAC1, a cocaine‐regulated POZ/BTB protein interacts with CoREST. Journal Of Neurochemistry 2007, 101: 611-618. PMID: 17254023, DOI: 10.1111/j.1471-4159.2006.04387.x.Peer-Reviewed Original ResearchConceptsPOZ/BTB proteinPOZ/BTB domainBTB proteinsBTB domainProtein-protein interactionsHEK293T cellsNeuro-2a cellsT cellsTranscriptional regulatorsRepressor proteinRat brain samplesBrain samplesCoRESTCoimmunoprecipitation studiesHomodimer formationHomodimer assemblyRepressor mechanismProtein expressionProteinDirect interactionEndogenous interactionPresent studyPox virus
2000
NAC-1 Is a Brain POZ/BTB Protein That Can Prevent Cocaine-Induced Sensitization in the Rat
Mackler S, Korutla L, Cha X, Koebbe M, Fournier K, Bowers M, Kalivas P. NAC-1 Is a Brain POZ/BTB Protein That Can Prevent Cocaine-Induced Sensitization in the Rat. Journal Of Neuroscience 2000, 20: 6210-6217. PMID: 10934270, PMCID: PMC6772573, DOI: 10.1523/jneurosci.20-16-06210.2000.Peer-Reviewed Original ResearchConceptsPOZ/BTB proteinBTB proteinsTranscriptional regulatorsNAC-1POZ/BTB domainZinc finger regionTwo-hybrid studiesDNA-binding proteinsBTB domainTranscription factorsGene transcriptionFinger regionReporter geneProteinNuclei of neuronsMammalian brainTranscriptionBehavioral sensitizationPox virusRegulatorCell culturesAdministration of cocaineRat nucleus accumbensExpressionLong-term neuroadaptations