2020
Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models
Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S, Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H. Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models. Cells 2020, 9: 1406. PMID: 32516938, PMCID: PMC7349756, DOI: 10.3390/cells9061406.Peer-Reviewed Original ResearchConceptsCell type-restricted expressionDisease-associated interactionsGene expression profilingHiPSC-derived motor neuronsHuman-induced pluripotent stem cellsPluripotent stem cellsCell-type specific perturbationsImmortalized cell linesRibosomal proteinsGenomic studiesExpression profilingMolecular mechanismsOff-target RNAMouse tissuesCell typesStem cellsPrimary mouse astrocytesExperimental replicatesCell linesMixed speciesMouse astrocytesExpressionMotor neuronsRiboTagCells
2019
Examining the relationship between astrocyte dysfunction and neurodegeneration in ALS using hiPSCs
Halpern M, Brennand KJ, Gregory J. Examining the relationship between astrocyte dysfunction and neurodegeneration in ALS using hiPSCs. Neurobiology Of Disease 2019, 132: 104562. PMID: 31381978, PMCID: PMC6834907, DOI: 10.1016/j.nbd.2019.104562.Peer-Reviewed Original ResearchConceptsAmyotrophic lateral sclerosisAstrocyte dysfunctionNeurodegenerative diseasesRole of astrocytesNon-cell autonomous mechanismsFatal neurodegenerative diseaseRisk-associated genesAstrocytic dysfunctionNeural cell typesAstrocyte functionDisease onsetDisease progressionMotor neuronsLateral sclerosisTherapeutic interventionsDysfunctionDisease initiationGenetic factorsPotential targetProgressionAutonomous mechanismsDiseaseStem cellsNeurodegenerationCell types