Using the dCas9-KRAB system to repress gene expression in hiPSC-derived NGN2 neurons
Li A, Cartwright S, Yu A, Ho SM, Schrode N, Deans PJM, Matos MR, Garcia MF, Townsley KG, Zhang B, Brennand KJ. Using the dCas9-KRAB system to repress gene expression in hiPSC-derived NGN2 neurons. STAR Protocols 2021, 2: 100580. PMID: 34151300, PMCID: PMC8188621, DOI: 10.1016/j.xpro.2021.100580.Peer-Reviewed Original ResearchConceptsCRISPR inhibitionGene expressionDCas9-KRAB systemEndogenous gene expressionMultiple target genesGene repressionGene activationTarget genesGene manipulationFusion proteinComplete detailsPluripotent stemExpressionGlutamatergic neuronsRepressionGenesPhenotypicProteinStemNeuronsActivationBrain diseasesInhibitionApplying stem cells and CRISPR engineering to uncover the etiology of schizophrenia
Michael Deans P, Brennand K. Applying stem cells and CRISPR engineering to uncover the etiology of schizophrenia. Current Opinion In Neurobiology 2021, 69: 193-201. PMID: 34010781, PMCID: PMC8387340, DOI: 10.1016/j.conb.2021.04.003.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCell type-specific fashionStem cell biologyType-specific fashionDisease-associated variantsNeural cell typesCommon genetic variantsMore genesCell biologyCRISPR engineeringGene manipulationGene targetsCRISPR technologyMolecular geneticsInvaluable advancesCell typesHiPSC technologyGenetic variantsStem cellsIndividual variantsEtiology of diseasePolygenic disorderVariantsComplex interactionsRecent advancesEtiology of schizophrenia