2019
Microfluidic platform enables live-cell imaging of signaling and transcription combined with multiplexed secretion measurements in the same single cells
Ramji R, Alexander AF, Muñoz-Rojas AR, Kellman LN, Miller-Jensen K. Microfluidic platform enables live-cell imaging of signaling and transcription combined with multiplexed secretion measurements in the same single cells. Integrative Biology 2019, 11: 142-153. PMID: 31242304, PMCID: PMC8672722, DOI: 10.1093/intbio/zyz013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCell CommunicationChemokine CCL2Chemokine CCL3Chemokine CCL5Equipment DesignLab-On-A-Chip DevicesLipopolysaccharidesMacrophagesMiceMice, Inbred C57BLMicrofluidicsRAW 264.7 CellsSignal TransductionTranscription Factor RelATranscription, GeneticTumor Necrosis Factor-alphaConceptsLive-cell imagingCell variabilitySame single cellSingle-cell assaysTranscription dynamicsBacterial component lipopolysaccharideDownstream responsesPathogenic assaultFluorescent reportersProtein secretionSingle cellsCell processesBiological sourcesCCL3 secretionRelative levelsCellsInnate immune cellsTranslocation dynamicsBiological stepC secretionTranscriptionSecretionCCL5 secretionRelAReporter
2016
Redefining Signaling Pathways with an Expanding Single-Cell Toolbox
Gaudet S, Miller-Jensen K. Redefining Signaling Pathways with an Expanding Single-Cell Toolbox. Trends In Biotechnology 2016, 34: 458-469. PMID: 26968612, PMCID: PMC4958913, DOI: 10.1016/j.tibtech.2016.02.009.Peer-Reviewed Original ResearchConceptsSignaling pathwaysSingle-cell toolsDNA damage responseCanonical signaling pathwaysSingle-cell measurementsNovel mechanistic insightsCell variabilityStress responseBiological informationAnalytical pipelineEnvironmental stimuliImaging-based approachIdentical cellsCell proliferationMechanistic insightsSingle cellsRecent technological advancesPathwayCellsUnderlying mechanismVivo applicabilityCell responsesResponseProliferationCell measurements
2012
Systems biology of virus-host signaling network interactions
Xue Q, Miller-Jensen K. Systems biology of virus-host signaling network interactions. BMB Reports 2012, 45: 213-220. PMID: 22531130, DOI: 10.5483/bmbrep.2012.45.4.213.Peer-Reviewed Original ResearchConceptsVirus-host protein interactionsHost cell machineryHost protein targetsSystems biology approachVirus-mediated changesBiology approachCell machineryProtein interactionsCell variabilityViral perturbationsSystems biologyBiological contextHost cellsMolecular studiesNovel therapeutic targetIndividual pathwaysViral pathogenesisNew therapeutic strategiesTherapeutic targetComputational analysisVirus propagationHostHost responseCellsRecent advances