2021
DMA-tudor interaction modules control the specificity of in vivo condensates
Courchaine EM, Barentine AES, Straube K, Lee DR, Bewersdorf J, Neugebauer KM. DMA-tudor interaction modules control the specificity of in vivo condensates. Cell 2021, 184: 3612-3625.e17. PMID: 34115980, PMCID: PMC8402948, DOI: 10.1016/j.cell.2021.05.008.Peer-Reviewed Original Research
2017
Cellular differentiation state modulates the mRNA export activity of SR proteins
Botti V, McNicoll F, Steiner MC, Richter FM, Solovyeva A, Wegener M, Schwich OD, Poser I, Zarnack K, Wittig I, Neugebauer KM, Müller-McNicoll M. Cellular differentiation state modulates the mRNA export activity of SR proteins. Journal Of Cell Biology 2017, 216: 1993-2009. PMID: 28592444, PMCID: PMC5496613, DOI: 10.1083/jcb.201610051.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsArginineCell DifferentiationCell NucleusDNA-Binding ProteinsHeLa CellsHumansImmunoprecipitationMethylationMiceNeurogenesisPhenotypePhosphorylationPluripotent Stem CellsProtein BindingProtein Processing, Post-TranslationalRepressor ProteinsRNA InterferenceRNA, MessengerRNA-Binding ProteinsSerine-Arginine Splicing FactorsTandem Mass SpectrometryTranscription FactorsTransfectionConceptsMRNA export activitySR proteinsP19 cellsMRNA exportSR protein family membersProtein-RNA interactionsMurine P19 cellsCellular differentiation stateProtein family membersLower phosphorylation levelsArginine methylationPluripotency factorsCytoplasmic mRNA levelsMRNA processingPosttranslational modificationsCellular dynamicsDifferentiated cellsNeural differentiationSRSF5Differentiation statePhosphorylation levelsHeLa cellsProteinExport activityMRNA levels
2015
Quantification of co-transcriptional splicing from RNA-Seq data
Herzel L, Neugebauer KM. Quantification of co-transcriptional splicing from RNA-Seq data. Methods 2015, 85: 36-43. PMID: 25929182, DOI: 10.1016/j.ymeth.2015.04.024.Peer-Reviewed Original Research
2014
The Coilin Interactome Identifies Hundreds of Small Noncoding RNAs that Traffic through Cajal Bodies
Machyna M, Kehr S, Straube K, Kappei D, Buchholz F, Butter F, Ule J, Hertel J, Stadler PF, Neugebauer KM. The Coilin Interactome Identifies Hundreds of Small Noncoding RNAs that Traffic through Cajal Bodies. Molecular Cell 2014, 56: 389-399. PMID: 25514182, DOI: 10.1016/j.molcel.2014.10.004.Peer-Reviewed Original ResearchConceptsCajal bodiesSmall nucleolar RNAsSmall nuclear RNASmall noncoding RNAsChIP-seq peaksCoilin proteinSnoRNA biogenesisRNP assemblySnRNP assemblyNcRNA metabolismSnRNA genesSubnuclear compartmentsCellular hubHuman snoRNAsMolecular functionsSnRNA transcriptsNucleolar RNAsNoncoding RNAsNuclear RNACoilinRNASignal trafficRole of CBAssemblySnRNAs
2012
The RNA-binding landscapes of two SR proteins reveal unique functions and binding to diverse RNA classes
Änkö ML, Müller-McNicoll M, Brandl H, Curk T, Gorup C, Henry I, Ule J, Neugebauer KM. The RNA-binding landscapes of two SR proteins reveal unique functions and binding to diverse RNA classes. Genome Biology 2012, 13: r17. PMID: 22436691, PMCID: PMC3439968, DOI: 10.1186/gb-2012-13-3-r17.Peer-Reviewed Original ResearchConceptsSR proteinsSR protein family membersHistone mRNA metabolismConsensus binding motifsProtein family membersIntron-containing mRNAsCellular regulatory mechanismsNonsense-mediated decayNon-coding RNAsNumerous RNAsHistone transcriptsMRNA metabolismUnique RNASplicing eventsAlternative splicingRNA classesRNA recognitionRelated RNATarget genesSRSF4Binding motifRegulatory mechanismsRNA targetsSRSF3Murine cells
2010
Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells
Änkö M, Morales L, Henry I, Beyer A, Neugebauer KM. Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells. Nature Structural & Molecular Biology 2010, 17: 962-970. PMID: 20639886, DOI: 10.1038/nsmb.1862.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell DifferentiationCell Line, TumorChromosomes, Artificial, BacterialGene Expression Regulation, NeoplasticGene Knockdown TechniquesGreen Fluorescent ProteinsImmunoprecipitationMiceNeuronsOrgan SpecificityProtein BindingRibonucleoproteinsRNA, MessengerRNA-Binding ProteinsSerine-Arginine Splicing Factors
1998
Regulation of Alternative Polyadenylation by U1 snRNPs and SRp20
Lou H, Neugebauer K, Gagel R, Berget S. Regulation of Alternative Polyadenylation by U1 snRNPs and SRp20. Molecular And Cellular Biology 1998, 18: 4977-4985. PMID: 9710581, PMCID: PMC109082, DOI: 10.1128/mcb.18.9.4977.Peer-Reviewed Original ResearchAnimalsBase SequenceCalcitoninCalcitonin Gene-Related PeptideConsensus SequenceEnhancer Elements, GeneticExonsHumansMetallothioneinMiceMolecular Sequence DataPoly ARatsRibonucleoprotein, U1 Small NuclearRNA PrecursorsRNA Processing, Post-TranscriptionalRNA-Binding ProteinsSequence AlignmentSequence Homology, Nucleic AcidSerine-Arginine Splicing FactorsThyroid GlandVertebrates
1995
A conserved epitope on a subset of SR proteins defines a larger family of Pre-mRNA splicing factors.
Neugebauer K, Stolk J, Roth M. A conserved epitope on a subset of SR proteins defines a larger family of Pre-mRNA splicing factors. Journal Of Cell Biology 1995, 129: 899-908. PMID: 7538140, PMCID: PMC2120486, DOI: 10.1083/jcb.129.4.899.Peer-Reviewed Original Research
1988
N-cadherin, NCAM, and integrins promote retinal neurite outgrowth on astrocytes in vitro.
Neugebauer K, Tomaselli K, Lilien J, Reichardt L. N-cadherin, NCAM, and integrins promote retinal neurite outgrowth on astrocytes in vitro. Journal Of Cell Biology 1988, 107: 1177-1187. PMID: 3262111, PMCID: PMC2115273, DOI: 10.1083/jcb.107.3.1177.Peer-Reviewed Original ResearchConceptsRetinal neuronsRetinal neurite outgrowthOptic pathwayCell adhesion moleculeNeurite outgrowthAdhesion moleculesEmbryonic chick retinal neuronsInhibitory effectRetinal ganglion neuronsChick retinal neuronsNeuronal cell surface proteinsN-cadherinDetectable inhibitory effectAttachment of neuronsEmbryonic day 7Ganglion neuronsDay 7Astrocyte monolayersIntegrin extracellular matrix receptorsNeuronsCell surface proteinsExtracellular matrix receptorsNCAMAstrocytesAxon extension