2020
Structural elucidation of the cis-prenyltransferase NgBR/DHDDS complex reveals insights in regulation of protein glycosylation
Edani BH, Grabińska KA, Zhang R, Park EJ, Siciliano B, Surmacz L, Ha Y, Sessa WC. Structural elucidation of the cis-prenyltransferase NgBR/DHDDS complex reveals insights in regulation of protein glycosylation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 20794-20802. PMID: 32817466, PMCID: PMC7456142, DOI: 10.1073/pnas.2008381117.Peer-Reviewed Original ResearchConceptsActive site tunnelProtein glycosylationAtomic resolution structuresGlycosyl carrier lipidsΑ3 helixEnzyme active sitePTase activityResolution structureActive siteEndoplasmic reticulumHomodimeric formCarrier lipidRate-limiting stepGlycosylationCrystal structureDHDDSStructural elucidationPTaseIsoprene chainPrenyltransferaseUnique insightsComplexesUnfavorable stateNgBRHomodimeric
1997
Polyprenol formation in the yeast Saccharomyces cerevisiae: effect of farnesyl diphosphate synthase overexpression
Szkopińska A, Grabińska K, Delourme D, Karst F, Rytka J, Palamarczyk G. Polyprenol formation in the yeast Saccharomyces cerevisiae: effect of farnesyl diphosphate synthase overexpression. Journal Of Lipid Research 1997, 38: 962-968. PMID: 9186913, DOI: 10.1016/s0022-2275(20)37220-5.Peer-Reviewed Original ResearchMeSH KeywordsAlkyl and Aryl TransferasesChromatography, High Pressure LiquidDolicholsGene Expression Regulation, FungalGeranyltranstransferaseSaccharomyces cerevisiaeTransferasesConceptsWild-type yeastType yeastSaccharomyces cerevisiaeYeast Saccharomyces cerevisiaeEffects of farnesylSynthesis of dolicholSame genetic backgroundHand overexpressionErg mutantsFPP synthaseSqualene synthase activitySynthesis of polyprenolsExogenous FPPMevalonate pathwayMutantsGenetic backgroundYeastOverexpressionSynthase overexpressionSynthase activityCerevisiaeFarnesylGenesSynthasePolyprenols