Featured Publications
The molecular basis of sugar detection by an insect taste receptor
Gomes J, Singh-Bhagania S, Cenci M, Chacon Cordon C, Singh M, Butterwick J. The molecular basis of sugar detection by an insect taste receptor. Nature 2024, 629: 228-234. PMID: 38447670, PMCID: PMC11062906, DOI: 10.1038/s41586-024-07255-w.Peer-Reviewed Original ResearchLigand-binding pocketGustatory receptorsD-fructoseMolecular basisStructure-guided mutagenesisSilkworm Bombyx moriPatterns of chemical groupsIon conductivitySugar selectivityAllosteric pathwaysBombyx moriExperimental binding assaysReceptor-ligand interactionsL-sorboseComputational dockingAromatic residuesActive ligandsAllosteric couplingConformational changesSugar detectionBinding assaysFunctional assaysChemical specificityChemical groupsTaste receptors
2006
An inserted Gly residue fine tunes dynamics between mesophilic and thermophilic ribonucleases H
Butterwick JA, Palmer AG. An inserted Gly residue fine tunes dynamics between mesophilic and thermophilic ribonucleases H. Protein Science 2006, 15: 2697-2707. PMID: 17088323, PMCID: PMC2242442, DOI: 10.1110/ps.062398606.Peer-Reviewed Original ResearchConceptsRNase HConformational changesGly residueMesophile Escherichia coliThermophile Thermus thermophilusSolution NMR spectroscopyIntrahelical hydrogen bondsHomologous mesophilicProtein sequencesBiological functionsThermus thermophilusImportant adaptationGly insertionPutative hingeEscherichia coliRibonuclease HMesophilicResiduesDynamic processUnfavorable interactionsThermophilesThermophilicProteinNMR spectroscopyThermophilus