2003
Splicing double: insights from the second spliceosome
Patel AA, Steitz JA. Splicing double: insights from the second spliceosome. Nature Reviews Molecular Cell Biology 2003, 4: 960-970. PMID: 14685174, DOI: 10.1038/nrm1259.Peer-Reviewed Original ResearchConceptsMinor class intronsPhylogenetic analysisU12-type intronsMost multicellular organismsSmall nuclear RNASmall nuclear ribonucleoproteinParticular functional classNon-homologous positionsMessenger RNA interactionsMost metazoan taxaParalogous genesMulticellular organismsSpliceosomal factorsHomologous genesEvolutionary historyMetazoan taxaU5 snRNPSplicing machineryAcceptor splice siteAlternative splicingRNA interactionsU6 snRNPsNuclear RNANuclear ribonucleoproteinSplicing reaction
1997
Inhibition of mammalian spliceosome assembly and pre-mRNA splicing by peptide inhibitors of protein kinases.
Parker AR, Steitz JA. Inhibition of mammalian spliceosome assembly and pre-mRNA splicing by peptide inhibitors of protein kinases. RNA 1997, 3: 1301-12. PMID: 9409621, PMCID: PMC1369569.Peer-Reviewed Original ResearchConceptsCalmodulin binding domainMammalian spliceosome assemblySpliceosome assemblyMRNA splicingSplicing activityCaMK IIGS peptideProtein kinase CAutophosphorylation eventsCalmodulin kinase IIProlonged incubationProtein kinaseSplicing reactionSplicing assaysBinding domainsKinase IISplicingKinase CPeptide inhibitorDistinct mechanismsProteinKinaseCompetitive inhibitorAssemblyDistinct events
1987
Identification of the Human U7 snRNP as One of Several Factors Involved in the 3′ End Maturation of Histone Premessenger RNA's
Mowry K, Steitz J. Identification of the Human U7 snRNP as One of Several Factors Involved in the 3′ End Maturation of Histone Premessenger RNA's. Science 1987, 238: 1682-1687. PMID: 2825355, DOI: 10.1126/science.2825355.Peer-Reviewed Original ResearchConceptsU7 snRNPPre-mRNAEnd processingDownstream elementsCleavage siteSmall nuclear ribonucleoprotein complexesMammalian pre-mRNAHeLa cell extractsNuclear ribonucleoprotein complexesHistone pre-mRNAEnd maturationEukaryotic cellsRibonucleoprotein complexesPremessenger RNARNA moietySplicing reactionGene transcriptsCell extractsSnRNPMessenger RNARNA
1981
Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing
Mount S, Steitz J. Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing. Nucleic Acids Research 1981, 9: 6351-6368. PMID: 6172778, PMCID: PMC327608, DOI: 10.1093/nar/9.23.6351.Peer-Reviewed Original ResearchConceptsU1 RNAU1 sequenceCultured Drosophila melanogaster cellsDrosophila U1 RNAU1 RNA sequenceDrosophila melanogaster cellsHuman U1 RNASecondary structure modelDNA sequence determinationDonor splice junctionGenomic clonesDrosophila melanogasterSplicing reactionRNA moleculesU1 snRNPsConsensus sequenceSplice junctionsRNA sequencesNucleotides 3Sequence determinationSecondary structureRNASplicingMolecular interactionsSequence