2016
Host miRNA degradation by Herpesvirus saimiri small nuclear RNA requires an unstructured interacting region
Pawlica P, Moss WN, Steitz JA. Host miRNA degradation by Herpesvirus saimiri small nuclear RNA requires an unstructured interacting region. RNA 2016, 22: 1181-1189. PMID: 27335146, PMCID: PMC4931111, DOI: 10.1261/rna.054817.115.Peer-Reviewed Original ResearchConceptsSmall nuclear RNAMiR-27Nuclear RNAVivo secondary structureBioinformatic structural analysisBinding site sequenceMiRNA degradationU RNARNA functionMutagenic analysisHSUR1Interacting regionsSite sequenceHost microRNAsSecondary structureHerpesvirus saimiriT cell activationOncogenic herpesvirusRNAStructural flexibilityDecreased levelsMutantsStructural analysisDegradationMicroRNAs
1994
Sequence and structural elements critical for U8 snRNP function in Xenopus oocytes are evolutionarily conserved.
Peculis BA, Steitz JA. Sequence and structural elements critical for U8 snRNP function in Xenopus oocytes are evolutionarily conserved. Genes & Development 1994, 8: 2241-2255. PMID: 7958892, DOI: 10.1101/gad.8.18.2241.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBiological EvolutionChromosomal Proteins, Non-HistoneConserved SequenceFemaleMethylationMolecular Sequence DataMutagenesis, Site-DirectedMutationNucleic Acid ConformationOocytesRibonucleoproteins, Small NuclearRNA CapsRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, Small NuclearXenopus
1993
The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1
Toczyski D, Steitz J. The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. DOI: 10.1128/mcb.13.1.703-710.1993.Peer-Reviewed Original ResearchSmall RNAsCellular RNA-binding proteinsConserved stem loopRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsDouble-stranded regionsDeletion mutantsEBER-1Mutational analysisStem loopFusion proteinProtection experimentsViral proteinsRNAProteinEpstein-Barr virus small RNAsEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinThe cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1.
Toczyski DP, Steitz JA. The cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. PMID: 8380232, PMCID: PMC358948, DOI: 10.1128/mcb.13.1.703.Peer-Reviewed Original ResearchConceptsCellular RNA-binding proteinsRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsEpstein‐Barr virus small RNA EBER‐1Double-stranded regionsSmall RNAsDeletion mutantsEBER-1Mutational analysisFusion proteinProtection experimentsViral proteinsProteinRNAEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinAntibodiesCells
1968
Identification of the A protein as a structural component of bacteriophage R17
Steitz J. Identification of the A protein as a structural component of bacteriophage R17. Journal Of Molecular Biology 1968, 33: 923-936. PMID: 4178187, DOI: 10.1016/0022-2836(68)90328-8.Peer-Reviewed Original ResearchConceptsA proteinBacteriophage R17Amber mutantsMolecule of histidineProtein monomersProtein productsMajor polypeptidesMolecular weight estimatesStructural componentsPhage particlesR17Non-permissive hostsDefective particlesVirus particlesProteinGel filtrationHistidineCistronMutantsSpheroplastsPolypeptideHostCopies