2023
Three-dimensional imaging of vascular development in the mouse epididymis.
Damon-Soubeyrand C, Bongiovanni A, Chorfa A, Goubely C, Pirot N, Pardanaud L, Piboin-Fragner L, Vachias C, Bravard S, Guiton R, Thomas J, Saez F, Kocer A, Tardivel M, Drevet J, Henry-Berger J. Three-dimensional imaging of vascular development in the mouse epididymis. ELife 2023, 12 PMID: 37310207, PMCID: PMC10264076, DOI: 10.7554/elife.82748.Peer-Reviewed Original ResearchConceptsProtection of spermatozoaComplex immune functionsAccessory tubulesVascular developmentMouse epididymisCellular levelSecretory roleImportant playersMale fertilityMale reproductive systemOrgan clearingMature adult miceReproductive systemTransgenic mouse modelFunctional maturationPeripheral toleranceBlood markersImmune responseLymphatic networkImmune functionKey determinantMouse modelSurvival of spermatozoaAdult miceImmune system
2019
Anatomy and function of the vertebral column lymphatic network in mice
Jacob L, Boisserand LSB, Geraldo LHM, de Brito Neto J, Mathivet T, Antila S, Barka B, Xu Y, Thomas JM, Pestel J, Aigrot MS, Song E, Nurmi H, Lee S, Alitalo K, Renier N, Eichmann A, Thomas JL. Anatomy and function of the vertebral column lymphatic network in mice. Nature Communications 2019, 10: 4594. PMID: 31597914, PMCID: PMC6785564, DOI: 10.1038/s41467-019-12568-w.Peer-Reviewed Original ResearchConceptsLymphatic vesselsCentral nervous system immune responseFocal spinal cord lesionsT cell infiltrationSpinal cord lesionsSpinal cord injuryCNS immunityCord lesionsMeningeal lymphatic vesselsSympathetic gangliaCord injuryCell infiltrationSpinal cordInflammatory responseEpidural spaceThoracic ductImmune responseDura materSpinal tissuePotential targetVertebral tissuesLymphatic networkSpine segmentsTraditional histologyLittle informationRNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells
Ghazale H, Ripoll C, Leventoux N, Jacob L, Azar S, Mamaeva D, Glasson Y, Calvo CF, Thomas JL, Meneceur S, Lallemand Y, Rigau V, Perrin FE, Noristani HN, Rocamonde B, Huillard E, Bauchet L, Hugnot JP. RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells. Stem Cell Reports 2019, 12: 1159-1177. PMID: 31031189, PMCID: PMC6524006, DOI: 10.1016/j.stemcr.2019.04.001.Peer-Reviewed Original ResearchConceptsTranscription factorsRNA profilingDevelopmental transcription factorsDorsal-ventral patternStem cell nicheEpendymal zoneMolecular resourcesMammalian lesionsConserved expressionCell nicheNeural stem cellsCell diversityPossible endogenous sourceQuiescent cellsGenesFloor plateStem cellsMsx1Endogenous sourcesTransgenic miceCellsProfilingSpinal cordCentral canalExpression
2017
Modulation of Endothelial Bone Morphogenetic Protein Receptor Type 2 Activity by Vascular Endothelial Growth Factor Receptor 3 in Pulmonary Arterial Hypertension
Hwangbo C, Lee HW, Kang H, Ju H, Wiley DS, Papangeli I, Han J, Kim JD, Dunworth WP, Hu X, Lee S, El-Hely O, Sofer A, Pak B, Peterson L, Comhair S, Hwang EM, Park JY, Thomas J, Bautch VL, Erzurum SC, Chun HJ, Jin SW. Modulation of Endothelial Bone Morphogenetic Protein Receptor Type 2 Activity by Vascular Endothelial Growth Factor Receptor 3 in Pulmonary Arterial Hypertension. Circulation 2017, 135: 2288-2298. PMID: 28356442, PMCID: PMC5523010, DOI: 10.1161/circulationaha.116.025390.Peer-Reviewed Original ResearchConceptsBMP receptor type 2Vascular endothelial growth factor receptor 3Growth factor receptor 3Zebrafish embryosPulmonary arterial endothelial cellsArterial endothelial cellsVEGFR3 expressionBone morphogenetic protein (BMP) signalingPulmonary arterial hypertensionMorphogenetic protein signalingEndothelial cellsFamilial pulmonary arterial hypertensionBMPR2 functionsPrimary lung endothelial cellsImpaired BMPBMP signalingBMP stimulationProtein signalingReceptor 3Endothelial-specific deletionEctopic angiogenesisKey regulatorHuman endothelial cellsArterial hypertensionLung endothelial cells
2013
Ascl1/Mash1 Promotes Brain Oligodendrogenesis during Myelination and Remyelination
Nakatani H, Martin E, Hassani H, Clavairoly A, Maire CL, Viadieu A, Kerninon C, Delmasure A, Frah M, Weber M, Nakafuku M, Zalc B, Thomas JL, Guillemot F, Nait-Oumesmar B, Parras C. Ascl1/Mash1 Promotes Brain Oligodendrogenesis during Myelination and Remyelination. Journal Of Neuroscience 2013, 33: 9752-9768. PMID: 23739972, PMCID: PMC3892435, DOI: 10.1523/jneurosci.0805-13.2013.Peer-Reviewed Original ResearchConceptsOligodendrocyte precursor cellsNeonatal periodCortical oligodendrocyte precursor cellsOligodendrocyte developmentCortical subventricular zoneSubventricular zone progenitorsMultiple sclerosis lesionsMyelin-forming cellsPostnatal cortexRemyelination processFocal demyelinationCorpus callosumSubventricular zoneOPC differentiationPostnatal brainMouse modelAscl1 functionOligodendrogenesisOPC developmentSclerosis lesionsASCL1 expressionCortical progenitorsRemyelinationProneural transcription factorsOligodendrocytes
2012
Semaphorin3A, Neuropilin-1, and PlexinA1 Are Required for Lymphatic Valve Formation
Bouvrée K, Brunet I, del Toro R, Gordon E, Prahst C, Cristofaro B, Mathivet T, Xu Y, Soueid J, Fortuna V, Miura N, Aigrot MS, Maden CH, Ruhrberg C, Thomas JL, Eichmann A. Semaphorin3A, Neuropilin-1, and PlexinA1 Are Required for Lymphatic Valve Formation. Circulation Research 2012, 111: 437-445. PMID: 22723296, PMCID: PMC3861899, DOI: 10.1161/circresaha.112.269316.Peer-Reviewed Original ResearchAnimalsAnimals, NewbornAntibodies, NeutralizingBacterial ProteinsCells, CulturedEndothelial CellsGene Expression Regulation, DevelopmentalGenotypeGestational AgeHumansLuminescent ProteinsLymphatic VesselsMiceMice, KnockoutMice, TransgenicMorphogenesisNerve Tissue ProteinsNeuropilin-1PhenotypeReceptors, Cell SurfaceRNA, MessengerSemaphorin-3AVascular Endothelial Growth Factor Receptor-3
2010
Neuropilin-2 mediates VEGF-C–induced lymphatic sprouting together with VEGFR3
Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivée B, del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A. Neuropilin-2 mediates VEGF-C–induced lymphatic sprouting together with VEGFR3. Journal Of Cell Biology 2010, 188: 115-130. PMID: 20065093, PMCID: PMC2812843, DOI: 10.1083/jcb.200903137.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ShapeCells, CulturedEndothelial CellsFemaleLymphangiogenesisLymphatic VesselsMaleMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicNeuropilin-2Protein BindingVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-2Vascular Endothelial Growth Factor Receptor-3ConceptsLymphatic vessel sproutingVEGF receptor 2Lymphangiogenic vascular endothelial growth factors CSprouting defectsNeuropilin-2Vessel sproutingVascular endothelial growth factor CVEGF-C bindingAntibody treatmentEndothelial tip cellsReceptor 2Lymph vesselsLymphatic sproutingGenetic deletionHeterozygous miceTransmembrane receptorsTip cellsAdult organsMiceCell extensionsNRP2Vascular systemVascular sprout formationVascular sproutingVEGF
2008
Genetic tracing of subpopulation neurons in the prethalamus of mice (Mus musculus)
Delaunay D, Heydon K, Miguez A, Schwab M, Nave K, Thomas J, Spassky N, Martinez S, Zalc B. Genetic tracing of subpopulation neurons in the prethalamus of mice (Mus musculus). The Journal Of Comparative Neurology 2008, 512: 74-83. PMID: 18973275, DOI: 10.1002/cne.21904.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementCell ShapeGenes, ReporterMiceMice, TransgenicNeuronsStem CellsThalamusConceptsVentral lateral geniculateCre/lox reporter systemGFP reporter miceGABAergic cellsGABAergic neuronsGlutamatergic neuronsLateral geniculateZona incertaHypothalamic nucleiSubthalamic nucleusReporter miceZona limitans intrathalamicaVentricular cellsIntergeniculate leafletNeuronsProgenitor cellsPosterior partGenetic tracingDiencephalonPool of progenitorsGenetic labelingPLP transcriptsMiceBasal plateCellsEarly Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells
Delaunay D, Heydon K, Cumano A, Schwab M, Thomas J, Suter U, Nave K, Zalc B, Spassky N. Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells. Journal Of Neuroscience 2008, 28: 2551-2562. PMID: 18322099, PMCID: PMC6671176, DOI: 10.1523/jneurosci.5497-07.2008.Peer-Reviewed Original ResearchConceptsGlial cellsEmbryonic neural stem cellsNeuronal progenitor cellsFate restrictionRadial glial cellsEmbryonic developmentNeural stem cellsNeuroepithelial progenitorsFate mappingNeuronal precursorsNeuroepithelial cellsNeurogenic periodStem cellsClonal analysisGlial precursorsProgenitor cellsGliogenic periodCellsProteolipid proteinNew poolDifferent time pointsLater stagesEmbryogenesis
2006
VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain
Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Bréant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Nature Neuroscience 2006, 9: 340-348. PMID: 16462734, DOI: 10.1038/nn1646.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCell DifferentiationCells, CulturedEvolution, MolecularIntermediate Filament ProteinsLarvaLateral VentriclesMiceMice, KnockoutMice, TransgenicNerve Growth FactorsNerve Tissue ProteinsNestinNeuronsOligodendrogliaOptic NerveRatsRats, WistarStem CellsVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-3Xenopus laevisConceptsNeural progenitor cellsReceptor VEGFR-3Mouse embryosNeural progenitorsVEGFR-3Progenitor cellsVertebrate embryonic brainBlood vessel defectsOligodendrocyte precursor cellsXenopus laevisAction of VEGFEmbryonic brainVascular endothelial growth factor CVEGF-C knockdownNeural cellsPrecursor cellsVessel defectsFactor CEmbryosGrowth factorProgenitorsCellsProliferation of OPCsVascular systemLymphatic vessels
2001
Sonic hedgehog-dependent emergence of oligodendrocytes in the telencephalon: evidence for a source of oligodendrocytes in the olfactory bulb that is independent of PDGFRalpha signaling.
Spassky N, Heydon K, Mangatal A, Jankovski A, Olivier C, Queraud-Lesaux F, Goujet-Zalc C, Thomas J, Zalc B. Sonic hedgehog-dependent emergence of oligodendrocytes in the telencephalon: evidence for a source of oligodendrocytes in the olfactory bulb that is independent of PDGFRalpha signaling. Development 2001, 128: 4993-5004. PMID: 11748136, DOI: 10.1242/dev.128.24.4993.Peer-Reviewed Original ResearchConceptsSpinal cordPlatelet-derived growth factor receptorOlfactory bulbOligodendrocyte progenitorsInduction of oligodendrocytesSource of oligodendrocytesAnterior entopeduncular areaMedial ganglionic eminenceExpression of PLPGrowth factor receptorRostral palliumTelencephalic oligodendrocytesGanglionic eminenceVentricular focusOligodendroglial lineageCordRostral telencephalonOligodendrocytesOligodendrocyte lineageFactor receptorVentricular progenitorsTelencephalonMouse telencephalonSonic hedgehogMolecular mechanisms
1998
Multiple Restricted Origin of Oligodendrocytes
Spassky N, Goujet-Zalc C, Parmantier E, Olivier C, Martinez S, Ivanova A, Ikenaka K, Macklin W, Cerruti I, Zalc B, Thomas J. Multiple Restricted Origin of Oligodendrocytes. Journal Of Neuroscience 1998, 18: 8331-8343. PMID: 9763477, PMCID: PMC6792828, DOI: 10.1523/jneurosci.18-20-08331.1998.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseBiomarkersBleomycinBrain ChemistryCell DifferentiationCell LineageCells, CulturedCentral Nervous SystemCloning, MolecularDNA-Binding ProteinsDrug Resistance, MicrobialFemaleGene Expression Regulation, DevelopmentalLac OperonMaleMiceMice, TransgenicNeuronsOligodendrogliaReceptors, Platelet-Derived Growth FactorStem CellsTranscription FactorsTransgenes
1997
In situ expression of PLP/DM‐20, MBP, and CNP during embryonic and postnatal development of the jimpy mutant and of transgenic mice overexpressing PLP
Peyron F, Timsit S, Thomas J, Kagawa T, Ikenaka K, Zalc B. In situ expression of PLP/DM‐20, MBP, and CNP during embryonic and postnatal development of the jimpy mutant and of transgenic mice overexpressing PLP. Journal Of Neuroscience Research 1997, 50: 190-201. PMID: 9373029, DOI: 10.1002/(sici)1097-4547(19971015)50:2<190::aid-jnr8>3.0.co;2-a.Peer-Reviewed Original ResearchConceptsPLP/DMCyclic nucleotide phosphodiesteraseDM-20PLP geneMyelin basic proteinDM-20 mRNAPattern of expressionEmbryonic developmentLevel of expressionJimpy mutantPeripheral nervous systemTransgenic micePrecursors of oligodendrocytesSpatiotemporal expressionDistinct poolsNeural tubeCentral nervous system