2023
Three-dimensional imaging of vascular development in the mouse epididymis.
Damon-Soubeyrand C, Bongiovanni A, Chorfa A, Goubely C, Pirot N, Pardanaud L, Piboin-Fragner L, Vachias C, Bravard S, Guiton R, Thomas J, Saez F, Kocer A, Tardivel M, Drevet J, Henry-Berger J. Three-dimensional imaging of vascular development in the mouse epididymis. ELife 2023, 12 PMID: 37310207, PMCID: PMC10264076, DOI: 10.7554/elife.82748.Peer-Reviewed Original ResearchConceptsProtection of spermatozoaComplex immune functionsAccessory tubulesVascular developmentMouse epididymisCellular levelSecretory roleImportant playersMale fertilityMale reproductive systemOrgan clearingMature adult miceReproductive systemTransgenic mouse modelFunctional maturationPeripheral toleranceBlood markersImmune responseLymphatic networkImmune functionKey determinantMouse modelSurvival of spermatozoaAdult miceImmune system
2020
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy.
Jacob L, Brito J, Thomas JL. Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy. Journal Of Visualized Experiments 2020 PMID: 32510513, DOI: 10.3791/61099.Peer-Reviewed Original ResearchConceptsLight sheet fluorescence microscopySheet fluorescence microscopyLymphatic vasculatureFluorescence microscopyCentral nervous systemLymphatic vesselsBiologyLymphatic systemLymphatic networkJoint biologyWhole-mount preparationsUnprecedented opportunityImmune surveillanceNervous systemImmune cellsTissueMount preparationsVertebral columnCNS tissueCellsMagnaMacromolecules
2019
Anatomy and function of the vertebral column lymphatic network in mice
Jacob L, Boisserand LSB, Geraldo LHM, de Brito Neto J, Mathivet T, Antila S, Barka B, Xu Y, Thomas JM, Pestel J, Aigrot MS, Song E, Nurmi H, Lee S, Alitalo K, Renier N, Eichmann A, Thomas JL. Anatomy and function of the vertebral column lymphatic network in mice. Nature Communications 2019, 10: 4594. PMID: 31597914, PMCID: PMC6785564, DOI: 10.1038/s41467-019-12568-w.Peer-Reviewed Original ResearchConceptsLymphatic vesselsCentral nervous system immune responseFocal spinal cord lesionsT cell infiltrationSpinal cord lesionsSpinal cord injuryCNS immunityCord lesionsMeningeal lymphatic vesselsSympathetic gangliaCord injuryCell infiltrationSpinal cordInflammatory responseEpidural spaceThoracic ductImmune responseDura materSpinal tissuePotential targetVertebral tissuesLymphatic networkSpine segmentsTraditional histologyLittle information