2024
Transcriptional regulation of postnatal aortic development
Weiss D, Yeung N, Ramachandra A, Humphrey J. Transcriptional regulation of postnatal aortic development. Cells And Development 2024, 180: 203971. PMID: 39426523, DOI: 10.1016/j.cdev.2024.203971.Peer-Reviewed Original ResearchMultiscale homogenized constrained mixture model of the bio-chemo-mechanics of soft tissue growth and remodeling
Paukner D, Humphrey J, Cyron C. Multiscale homogenized constrained mixture model of the bio-chemo-mechanics of soft tissue growth and remodeling. Biomechanics And Modeling In Mechanobiology 2024, 23: 2115-2136. PMID: 39419845, PMCID: PMC11554721, DOI: 10.1007/s10237-024-01884-w.Peer-Reviewed Original ResearchConstrained mixture modelsNonlinear continuum mechanicsSoft biological tissuesChemo-mechanical interactionsSolid mechanicsChemo-mechanical couplingContinuum mechanicsOrdinary differential equationsSignal processingBiological tissuesMixture modelDifferential equationsEquationsSimulate many casesTissue growthOrgan-scaleComputational analysis of heart valve growth and remodeling after the Ross procedure
Middendorp E, Braeu F, Baaijens F, Humphrey J, Cyron C, Loerakker S. Computational analysis of heart valve growth and remodeling after the Ross procedure. Biomechanics And Modeling In Mechanobiology 2024, 23: 1889-1907. PMID: 39269523, PMCID: PMC11554944, DOI: 10.1007/s10237-024-01874-y.Peer-Reviewed Original ResearchRoss procedureBlood pressure controlRoot dilatationHomogeneous mixture modelPatient's own pulmonary valveMechanical homeostasisPressure controlAortic heart valvesPublished clinical studiesConstrained mixture modelsHemodynamic environmentPulmonary autograftPulmonary valveLeaflet elongationPressure conditionsClinical studiesG&RHemodynamic loadTissue compositionValve growthTissue depositionMixture modelDilatationHeart valvesAutograftMechanisms of Aortic Dissection: From Pathological Changes to Experimental and In Silico Models
Rolf-Pissarczyk M, Schussnig R, Fries T, Fleischmann D, Elefteriades J, Humphrey J, Holzapfel G. Mechanisms of Aortic Dissection: From Pathological Changes to Experimental and In Silico Models. Progress In Materials Science 2024, 101363. DOI: 10.1016/j.pmatsci.2024.101363.Peer-Reviewed Original ResearchAT1b receptors contribute to regional disparities in angiotensin II mediated aortic remodelling in mice
Cavinato C, Spronck B, Caulk A, Murtada S, Humphrey J. AT1b receptors contribute to regional disparities in angiotensin II mediated aortic remodelling in mice. Journal Of The Royal Society Interface 2024, 21: 20240110. PMID: 39192727, PMCID: PMC11350382, DOI: 10.1098/rsif.2024.0110.Peer-Reviewed Original ResearchConceptsAngiotensin II infusionAT1B receptorsII infusionAngiotensin IIChronic angiotensin II infusionDays of angiotensin II infusionWild-typeAortas of WTSmooth muscle contractilityDescending thoracic aortaAngiotensin II signalingInflammatory cell responseRenin-angiotensin systemRegulating blood pressureAngII infusionAortic remodelingCell biological changesImmunohistological changesIndependent of sexMuscle contractilityMale miceMouse modelAbdominal aortaArterial remodelingBlood pressureCellular stiffness sensing through talin 1 in tissue mechanical homeostasis
Chanduri M, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz U, Bai S, Nottoli T, Goult B, Humphrey J, Schwartz M. Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis. Science Advances 2024, 10: eadi6286. PMID: 39167642, PMCID: PMC11338229, DOI: 10.1126/sciadv.adi6286.Peer-Reviewed Original ResearchConceptsTissue mechanical homeostasisStiffness sensingExtracellular matrixTalin-1Mechanical homeostasisExtracellular matrix mechanicsIncreased cell spreadingCell spreadingTalinMutationsCellular sensingFibrillar collagenReduced axial stiffnessTissue mechanical propertiesMechanical propertiesAxial stiffnessCompliant substratesHomeostasisRupture pressureArp2/3ARPC5LStiffnessHomeostasis hypothesisResident cellsTissue stiffnessFSGe: A fast and strongly-coupled 3D fluid–solid-growth interaction method
Pfaller M, Latorre M, Schwarz E, Gerosa F, Szafron J, Humphrey J, Marsden A. FSGe: A fast and strongly-coupled 3D fluid–solid-growth interaction method. Computer Methods In Applied Mechanics And Engineering 2024, 431: 117259. PMID: 39430055, PMCID: PMC11484312, DOI: 10.1016/j.cma.2024.117259.Peer-Reviewed Original ResearchWall shear stressG&R modelIntramural stressVariation of wall shear stressHyperelastic material modelNavier-Stokes equationsFluid-solid-growthMaterial modelMaterial propertiesShear stressSimulation resultsThree-dimensionalComputational examplesComputational costEvolving geometryG&RWall adaptationLocal variationsFluid solutionStressMechanics and mechanobiology of intracranial aneurysms
Humphrey J. Mechanics and mechanobiology of intracranial aneurysms. Mathematics And Mechanics Of Solids 2024 DOI: 10.1177/10812865231219163.Peer-Reviewed Original ResearchDynamic biaxial loading of vascular smooth muscle cell seeded tissue equivalents
Paukner D, Jennings I, Cyron C, Humphrey J. Dynamic biaxial loading of vascular smooth muscle cell seeded tissue equivalents. Journal Of The Mechanical Behavior Of Biomedical Materials 2024, 157: 106639. PMID: 38970943, DOI: 10.1016/j.jmbbm.2024.106639.Peer-Reviewed Original ResearchCyclic loadingBiaxial loading conditionsPeriod of cyclic loadingStudy cell-matrix interactionsLoad amplitudeUniaxial experimentsDynamic loadingBiaxial forceCruciform samplesLoading conditionsExternal loadMechanical stateBoundary conditionsLoadExtracellular matrixInfluence cell phenotypeMechanical constraintsRange of forcesCell-matrix interactionsCollagen gelsTissue equivalentsForceSynthetic cellsLoad in vivoVascular smooth muscle cellsStiffening of the human proximal pulmonary artery with increasing age
Manning E, Mishall P, Ramachandra A, Hassab A, Lamy J, Peters D, Murphy T, Heerdt P, Singh I, Downie S, Choudhary G, Tellides G, Humphrey J. Stiffening of the human proximal pulmonary artery with increasing age. Physiological Reports 2024, 12: e16090. PMID: 38884325, PMCID: PMC11181131, DOI: 10.14814/phy2.16090.Peer-Reviewed Original ResearchConceptsProximal pulmonary arteriesPulmonary arteryAge-related stiffeningRight ventricular ejection fractionVentricular ejection fractionMean wall thicknessEjection fractionNo significant differenceSystemic circulationLuminal caliberDiffusing capacityArterial stiffeningIncreasing ageSignificant differenceDistensionArteryWall thicknessAdverse effectsWall strainVessel diameterCyclic biaxial strainOrgan donorsAgeMicrostructural remodelingCell signaling and tissue remodeling in the pulmonary autograft after the Ross procedure: A computational study
Maes L, Vervenne T, Hendrickx A, Estrada A, Van Hoof L, Verbrugghe P, Rega F, Jones E, Humphrey J, Famaey N. Cell signaling and tissue remodeling in the pulmonary autograft after the Ross procedure: A computational study. Journal Of Biomechanics 2024, 171: 112180. PMID: 38906711, DOI: 10.1016/j.jbiomech.2024.112180.Peer-Reviewed Original ResearchRoss procedurePulmonary autograftPatient's pulmonary valveTranscription factorsPulmonary valveSmooth muscleActivation of genesAortic positionRelevant transcription factorsTissue remodelingAutograftExcessive dilatationTranscriptome dataGene activationCell signalingSignaling pathwayTarget genesMultiscale modelGenesTissueCellsFailure mechanismCell-scaleMechanical propertiesCell-scale modelMultiscale computational model of aortic remodeling following postnatal disruption of TGFβ signaling
Estrada A, Irons L, Tellides G, Humphrey J. Multiscale computational model of aortic remodeling following postnatal disruption of TGFβ signaling. Journal Of Biomechanics 2024, 169: 112152. PMID: 38763809, PMCID: PMC11141772, DOI: 10.1016/j.jbiomech.2024.112152.Peer-Reviewed Original ResearchAdult aortaTGFB signalingSmooth muscle cellsAortic remodelingCardiac-inducedMouse modelNormal mechanical loadingMuscle cellsPostnatal developmentHemodynamic loadNormal loadAortaMechanical homeostasisMechanical loadingMultiscale computational modelIncreasing loadLoadCell signalingGene productsStructural integrityHemodynamics and Wall Mechanics of Vascular Graft Failure
Szafron J, Heng E, Boyd J, Humphrey J, Marsden A. Hemodynamics and Wall Mechanics of Vascular Graft Failure. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: 1065-1085. PMID: 38572650, PMCID: PMC11043008, DOI: 10.1161/atvbaha.123.318239.Peer-Reviewed Original ResearchConceptsVascular graftsTissue-engineered vascular graftsWall mechanicsSolid mechanicsVascular graft failureLoad magnitudeMechanobiological processesLoadMechanobiological stimuliMechanosensitive signaling pathwaysBiomechanical stateWallGraft failureBiomechanical loadingCongenital heart surgeryCoronary artery bypass graftingEnd-organ dysfunctionGraft materialArtery bypass graftingFeedback loopComputational toolsRemodeling of Murine Branch Pulmonary Arteries Under Chronic Hypoxia and Short-Term Normoxic Recovery.
Ramachandra A, Jiang B, Jennings I, Manning E, Humphrey J. Remodeling of Murine Branch Pulmonary Arteries Under Chronic Hypoxia and Short-Term Normoxic Recovery. Journal Of Biomechanical Engineering 2024, 146 PMID: 38421341, DOI: 10.1115/1.4064967.Peer-Reviewed Original ResearchWeeks of hypoxiaBranch pulmonary arteriesWeeks of normoxic recoveryPulmonary arteryEndothelial cell responsesSmooth muscleChronic hypoxiaNormoxic recoveryPulmonary arterial remodelingCell responsesProximal pulmonary arteriesLongitudinal changesHypoxia-induced changesC57BL/6J micePulmonary pathologyThin collagen fibersArterial remodelingBiaxial mechanicsCircumferential stressEnergy storageNormal valuesHypoxiaWeeksVascular adaptationFraction of cytoplasmTissue engineered vascular grafts are resistant to the formation of dystrophic calcification
Turner M, Blum K, Watanabe T, Schwarz E, Nabavinia M, Leland J, Villarreal D, Schwartzman W, Chou T, Baker P, Matsumura G, Krishnamurthy R, Yates A, Hor K, Humphrey J, Marsden A, Stacy M, Shinoka T, Breuer C. Tissue engineered vascular grafts are resistant to the formation of dystrophic calcification. Nature Communications 2024, 15: 2187. PMID: 38467617, PMCID: PMC10928115, DOI: 10.1038/s41467-024-46431-4.Peer-Reviewed Original ResearchConceptsTissue-engineered vascular graftsTissue engineered vascular graftsVascular graftsCongenital heart surgeryComputational fluid dynamics simulationsFluid dynamics simulationsPolytetrafluoroethylene graftHeart surgeryDystrophic calcificationRetrospective clinical studyGraft complianceDegree of calcificationClinical studiesProsthetic biomaterialsBiomaterialsAnimal modelsPolytetrafluoroethyleneHemodynamic performanceNatural historyCalcificationGraftOvine modelVascular conduitsLong-term successSurgeryCentral Artery Hemodynamics in Angiotensin II-Induced Hypertension and Effects of Anesthesia
Hopper S, Weiss D, Mikush N, Jiang B, Spronck B, Cavinato C, Humphrey J, Figueroa C. Central Artery Hemodynamics in Angiotensin II-Induced Hypertension and Effects of Anesthesia. Annals Of Biomedical Engineering 2024, 52: 1051-1066. PMID: 38383871, PMCID: PMC11418744, DOI: 10.1007/s10439-024-03440-0.Peer-Reviewed Original ResearchEffects of angiotensin II infusionMouse-to-mouse variationAngiotensin II-induced hypertensionRegional vascular structuresII-induced hypertensionAngiotensin II-infused miceAngiotensin II infusionWild-type miceDescending thoracic aortaGroups of miceSuprarenal abdominal aortaEffects of hypertensionCentral arterial stiffnessDepressed hemodynamicsII infusionIndicator of hypertensionSystemic hypertensionRenovascular diseaseCardiac functionInduced hypertensionDistal aortaThoracic aortaEffects of anesthesiaMouse modelAbdominal aortaGrand Challenges at the Interface of Engineering and Medicine
Subramaniam S, Akay M, Anastasio M, Bailey V, Boas D, Bonato P, Chilkoti A, Cochran J, Colvin V, Desai T, Duncan J, Epstein F, Fraley S, Giachelli C, Grande-Allen K, Green J, Guo X, Hilton I, Humphrey J, Johnson C, Karniadakis G, King M, Kirsch R, Kumar S, Laurencin C, Li S, Lieber R, Lovell N, Mali P, Margulies S, Meaney D, Ogle B, Palsson B, Peppas N, Perreault E, Rabbitt R, Setton L, Shea L, Shroff S, Shung K, Tolias A, van der Meulen M, Varghese S, Vunjak-Novakovic G, White J, Winslow R, Zhang J, Zhang K, Zukoski C, Miller M. Grand Challenges at the Interface of Engineering and Medicine. IEEE Open Journal Of Engineering In Medicine And Biology 2024, 5: 1-13. PMID: 38415197, PMCID: PMC10896418, DOI: 10.1109/ojemb.2024.3351717.Peer-Reviewed Original ResearchDevelopmental changes in lung function of mice are independent of sex as a biological variable
Bärnthaler T, Ramachandra A, Ebanks S, Guerrera N, Sharma L, Dela Cruz C, Humphrey J, Manning E. Developmental changes in lung function of mice are independent of sex as a biological variable. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2024, 326: l627-l637. PMID: 38375577, PMCID: PMC11380952, DOI: 10.1152/ajplung.00120.2023.Peer-Reviewed Original ResearchPulmonary function testsWeeks of ageStatic complianceMale miceInspiratory capacityLung functionBody weight-basedHuman lung pathologiesLung function of miceFemale C57BL/6J miceLung function parametersSex-dependent changesFunction of miceMeasures of lung functionIndependent of sexPreclinical modelsFemale miceC57BL/6J miceLung pathologyFunction testsMiceWeight-basedLungLung disease researchWeeksA Systematic Comparison of Normal Structure and Function of the Greater Thoracic Vessels
Ramachandra A, Cavinato C, Humphrey J. A Systematic Comparison of Normal Structure and Function of the Greater Thoracic Vessels. Annals Of Biomedical Engineering 2024, 52: 958-966. PMID: 38227167, DOI: 10.1007/s10439-023-03432-6.Peer-Reviewed Original ResearchThoracic vesselsVena cavaThoracic aortaCongenital heart surgeryRight pulmonary arterySuperior vena cavaInferior vena cavaRight subclavian arteryThoracic inferior vena cavaWild-type miceAscending thoracic aortaDescending thoracic aortaPulmonary arterySubclavian arteryHeart surgerySurgical interventionVasoactive responsesVenous circulationThoracicCavaNormal structureAortaArteryBiomechanical propertiesVascular systemChapter 25 Pharmacological treatments, mouse models, and the aorta
Humphrey J. Chapter 25 Pharmacological treatments, mouse models, and the aorta. 2024, 569-592. DOI: 10.1016/b978-0-323-95484-6.00021-x.Peer-Reviewed Original Research