1999
Canalicular Export Pumps Traffic with Polymeric Immunoglobulin A Receptor on the Same Microtubule-associated Vesicle in Rat Liver*
Soroka C, Pate M, Boyer J. Canalicular Export Pumps Traffic with Polymeric Immunoglobulin A Receptor on the Same Microtubule-associated Vesicle in Rat Liver*. Journal Of Biological Chemistry 1999, 274: 26416-26424. PMID: 10473600, DOI: 10.1074/jbc.274.37.26416.Peer-Reviewed Original ResearchConceptsApical membrane proteinsMembrane proteinsMicrotubule-associated vesiclesSite of functionMembrane transport proteinsMembrane-bound vesiclesNon-hydrolyzable nucleotidesReceptor trafficMicrotubule motorsTransport proteinsIntermediate chainEssential pathwayPolymeric immunoglobulinsSame vesiclesTranscytotic vesiclesMultidrug resistance protein 2Protein 2Immunoelectron microscopyVesiclesMicrotubulesProteinVesicular transcytosisPathwayRat hepatocytesCanalicular export
1995
Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets
Boyer J, Soroka C. Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets. Gastroenterology 1995, 109: 1600-1611. PMID: 7557144, DOI: 10.1016/0016-5085(95)90649-5.Peer-Reviewed Original ResearchConceptsInsertion of vesiclesTranslocation of vesiclesMembrane transport activityVesicle trafficApical domainIsolated rat hepatocyte coupletsAcid transportersTransport activityNocodazoleRat hepatocyte coupletsVesiclesIndependent mechanismsEpithelial cellsBile acid transporterSite of residenceHepatocyte coupletsTransportersCanalicular membraneRat hepatocytesImmunocytochemical localizationCyclic monophosphateAdenosine triphosphataseBile excretory function
1994
Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes.
Benedetti A, Strazzabosco M, Ng O, Boyer J. Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 792-796. PMID: 8290601, PMCID: PMC43035, DOI: 10.1073/pnas.91.2.792.Peer-Reviewed Original ResearchConceptsApical targetingExchange activityRegulation of activityMembrane localizationCl-/HCO3Dibutyryl cAMPProtein kinase C agonistsRat hepatocytesProtein kinase C agonist phorbolEcto-ATPaseConfocal microscopyCanalicular domainC agonistsProteinVesiclesTargetingCanalicular localizationLocalizationCAMPHepatocyte coupletsHepatocytesActivityRegulationPhorbolColchicine
1991
Mechanism of mercurial inhibition of sodium-coupled alanine uptake in liver plasma membrane vesicles from Raja erinacea
Sellinger M, Ballatori N, Boyer J. Mechanism of mercurial inhibition of sodium-coupled alanine uptake in liver plasma membrane vesicles from Raja erinacea. Toxicology And Applied Pharmacology 1991, 107: 369-376. PMID: 1994517, DOI: 10.1016/0041-008x(91)90216-2.Peer-Reviewed Original ResearchConceptsDependent alanine uptakePlasma membrane vesiclesMembrane vesiclesAlanine uptakePrimitive vertebratesPlasma membraneTransport proteinsConcentration-dependent mechanismLiver plasma membrane vesiclesMicroM HgCl2Mammalian hepatocytesAlanine carrierSulfhydryl groupsLittle skateMembrane integrityRaja erinaceaDirect interactionVesiclesVesicle integrityMembrane permeabilityMercurial inhibitionUptake rateDose-dependent mannerHgCl2 concentrationsLiver plasma membranes
1987
Anion transport in basolateral (sinusoidal) liver plasma-membrane vesicles of the little skate (Raja erinacea)
Hugentobler G, Fricker G, Boyer J, Meier P. Anion transport in basolateral (sinusoidal) liver plasma-membrane vesicles of the little skate (Raja erinacea). Biochemical Journal 1987, 247: 589-595. PMID: 3426551, PMCID: PMC1148453, DOI: 10.1042/bj2470589.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBiological TransportCarbonyl Cyanide m-Chlorophenyl HydrazoneCell MembraneCholic AcidCholic AcidsElectric FishHydrogen-Ion ConcentrationIn Vitro TechniquesLiverMaleProbenecidSkates, FishSulfatesConceptsPlasma membrane vesiclesBasolateral liver plasma membrane vesiclesSulfate uptakeLiver plasma membrane vesiclesProtonophore carbonyl cyanideSulfate transport systemVertebrate evolutionMammalian liver cellsPH-equilibrated conditionsTransmembrane transportPH gradientLittle skateRaja erinaceaUptake 5Carbonyl cyanideCI inhibitionAnion transport inhibitorAlkaline pH gradientVesiclesAnion transportSkate hepatocytesCertain organic anionsUptakeLiver cellsElasmobranchs
1985
The effect of ethanol and calcium on fluid state of plasma membranes of rat hepatocytes
Mills P, Meier P, Boyer J, Gordon E. The effect of ethanol and calcium on fluid state of plasma membranes of rat hepatocytes. Alcohol 1985, 2: 153-156. PMID: 4015830, DOI: 10.1016/0741-8329(85)90034-5.Peer-Reviewed Original ResearchConceptsMembrane protein functionProtein functionMembrane fluidityCanalicular plasma membrane vesiclesPlasma membrane vesiclesOverall cellular activityMembrane lipid fluidityPlasma membraneMembrane preparationsMembrane vesiclesCellular activitiesImportant regulatorLipid fluidityMembrane perturbantsFluorescence polarizationRat hepatocytesFluidityBlLPMRegulatorUptakeUptake of taurocholateVesiclesMillipore filtration technique